A5068513225- Hematopoietic Stem Cell Transplantation
- Mesenchymal stem cell research
- Electrospun Nanofibers in Biomedical Applications
- 3D Printing in Biomedical Research
- Tissue Engineering and Regenerative Medicine
- CAR-T cell therapy research
- Acute Myeloid Leukemia Research
- Cancer Cells and Metastasis
- Esophageal and GI Pathology
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Spinal Dysraphism and Malformations
- Single-cell and spatial transcriptomics
- Cancer Genomics and Diagnostics
- Neonatal Respiratory Health Research
- Congenital Diaphragmatic Hernia Studies
- Hematological disorders and diagnostics
- Angiogenesis and VEGF in Cancer
- Platelet Disorders and Treatments
- Congenital Anomalies and Fetal Surgery
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Proteoglycans and glycosaminoglycans research
- Corneal Surgery and Treatments
- Immune Cell Function and Interaction
- Wound Healing and Treatments
Université Paris Cité
2014-2024
Inserm
2016-2024
Assistance Publique – Hôpitaux de Paris
2016-2024
Hôpital Saint-Louis
2016-2024
University of Prishtina
2023
Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes
2023
Laboratoire d’immunologie intégrative du cancer
2022
Institut de recherche Saint-Louis
2019-2021
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2020
Ecotaxie, Microenvironnement et développement lymphocytaire
2016
Tissue engineering holds great promise for regenerative medicine, drug discovery, and as an alternative to animal models. However, soon the dimensions of engineered tissue exceed diffusion limit oxygen nutriments, a necrotic core forms leading irreversible damage. To overcome this constraint, establishment functional perfusion network is essential. In work, digital light processing bioprinting used encapsulate endothelial progenitor cells (EPCs) in 3D light-cured hydrogel scaffolds guide...
à la diffusion de documents scientifiques niveau recherche, publiés ou non, émanant des établissements d'enseignement et recherche français étrangers, laboratoires publics privés.
Abstract Hematopoiesis develops in the bone marrow (BM) where multiple interactions regulate differentiation and preservation of hematopoietic stem progenitor cells (HSPCs). Immune‐deficient murine models have enabled analysis molecular cellular regulation human HSPCs, but physiology these is questioned as develop xenogenic microenvironments. In this study, we thoroughly characterized a humanized (h) vivo BM model, developed from fetal (F/) post‐natal (P‐N/) mesenchymal stromal cell (MSC)...
Abstract Background Umbilical cord-derived mesenchymal stromal cells (UC-MSCs) revealed their key role in immune regulation, offering promising therapeutic perspectives for and inflammatory diseases. We aimed to develop a production process of an UC-MSC-based product then characterize UC-MSC properties immunomodulatory activities vitro, related clinical use finally, transfer this technology good manufacturing practice (GMP) compliant facility, manufacture advanced therapy medicinal (ATMP)....
Abstract Background Mesenchymal stem/stromal cells (MSC) have immunomodulatory properties, studied in a wide range of diseases. Validated quality controls must confirm this activity the context clinical trials. This study presents method’s validation, assessing MSC’s ability to inhibit lymphocyte proliferation, according ICH Q2 standard. Methods MSC were co-cultured with CellTrace™ Violet-labeled Peripheral blood mononuclear (PBMC) coming from bank ten donors, at seven different ratios for 7...
In pathologies of the esophagus such as esophageal atresia, cancers, and caustic injuries, methods for full thickness replacement require sacrifice healthy intra-abdominal organs stomach colon are associated with high morbidity, mortality, poor functional results. To overcome these problems, tissue engineering developed to create a substitute scaffolds cells. The aim this study was develop simple safe decellularization process in order obtain clinical grade extracellular matrix. Following...
Many tissue engineering approaches are being explored to offer solutions for diseased esophageal and its repair. However, classical techniques in not capable of recapitulating the structural functional parameters native esophagi. Esophageal 3D bioprinting is yet an emerging field but holds great promise meeting this challenge. Herein, use extrusion‐based printing examined generate substitutes from a polymer blend‐based formulation. The fabricated structures printed at very high speed without...
The fate of hematopoietic stem and progenitor cells (HSPCs) is regulated by their interaction with stromal in the bone marrow. However, cellular mechanisms regulating HSPC these potential impact on polarity are still poorly understood. Here we evaluated cell–cell contacts osteoblasts or endothelial HSPC. We found that an can form a discrete contact site leads to extensive polarization its cytoskeleton architecture. Notably, centrosome was located proximity site. capacity HSPCs polarize...
Sustained ANKRD26 expression associated with germline mutations causes thrombocytopenia 2 (THC2), an inherited platelet disorder a predisposition to leukemia. Some patients also present erythrocytosis and/or leukocytosis. Using multiple human-relevant in vitro models (cell lines, primary patients’ cells and patient-derived induced pluripotent stem cells) we demonstrate for the first time that is expressed during early steps of erythroid, megakaryocyte granulocyte differentiation, necessary...
Hematopoietic stem and progenitor cells (HSPCs) give rise to all cell types of the hematopoietic system through various processes, including asymmetric divisions. However, contribution stromal niches in control HSPC divisions remains unknown. Using polyacrylamide microwells as minimalist niches, we show that specific heterotypic interactions with osteoblast endothelial promote human HSPCs. Upon interaction, HSPCs polarize interphase centrosome, Golgi apparatus, lysosomes positioned close...
For the last 40 years, technique of extracorporeal photopheresis (ECP) has constantly developed. Among irradiation systems, those called "off-line" allow validation quality cell therapy product. The inhibition proliferation lymphocytes after ultraviolet (UVA) is usually verified by tritiated thymidine assay as in vitro assay. document presented here describes results obtained while performing setting up an alternative using flow cytometry according to ISO 15189:2007 Standard.Cells samples...
Abstract Background Expansion protocols aim at both increasing the number of umbilical cord blood (UCB) hematopoietic stem cells and progenitor (HSPCs) reducing period neutropenia in UCB HSPC graft. Because glycosaminoglycans (GAGs) are known to be important components niche modulate growth factor effects, we explored use GAG mimetic OTR4131 potentiate HSPC’s vitro expansion vivo engraftment. Methods CD34+ were expanded with serum-free medium, SCF, TPO, FLT3-lig G-CSF during 12 days absence...
ABSTRACT Hematopoietic stem cells (HSC) can differentiate into all hematopoietic lineages to support hematopoiesis. Cells from the myeloid and lymphoid fulfill distinct functions with specific shapes intra-cellular architectures. The role of cytokines in regulation HSC differentiation has been intensively studied but our understanding potential contribution inner cell architecture is relatively poor. Here we show that large invaginations are generated by microtubule constraints on swelling...
Background: For the last 40 years, technique of extracorporeal photopheresis has constantly developed. Among irradiation systems, those called 'off-line' allow validation quality cell therapy product. The inhibition proliferation lymphocytes after UVA is usually verified by tritiated thymidine assay as in vitro assay. document presented here describes results obtained while performing setting up an alternative using flow cytometry according to ISO 15189:2007 Standard. Methods: Cells samples...
Abstract Hematopoietic Stem and Progenitor Cells (HSPCs) give rise to all cell types of the hematopoietic system through various processes including asymmetric divisions. However, contribution stromal cells niches in control HSPC divisions remains unknown. Using polyacrylamide microwells as minimalist niches, we show that heterotypic interaction with osteoblast promotes division human HSPC. Upon interaction, HSPCs polarize interphase centrosome, Golgi apparatus lysosomes positioned close...
Tissue engineering is a promising alternative to current full thickness circumferential esophageal replacement methods. The aim of our study was develop clinical grade Decellularized Human Esophagus (DHE) for future applications. After decontamination, human esophagi from deceased donors were placed in bioreactor and decellularized with sodium dodecyl sulfate (SDS) ethylendiaminetetraacetic acid (EDTA) 3 days. then rinsed sterile water SDS eliminated by filtration on an activated charcoal...
Abstract Background Myelomeningocele (MMC) is a spinal cord congenital defect that leads to paraplegia, sphincter disorders and potential neurocognitive disabilities. Prenatal surgery of MMC provides significant benefit compared at birth. Mesenchymal stromal cell (MSC) therapy as an adjuvant treatment for prenatal showed promising results in animal experiments which could be considered clinical use human fetuses. Despite numerous reassuring studies on the safety MSCs administration humans,...