A5055542572- Metabolism, Diabetes, and Cancer
- Genomic variations and chromosomal abnormalities
- Pluripotent Stem Cells Research
- Prenatal Screening and Diagnostics
- Growth Hormone and Insulin-like Growth Factors
- CRISPR and Genetic Engineering
- Ovarian function and disorders
- Adipokines, Inflammation, and Metabolic Diseases
- Reproductive Biology and Fertility
- Congenital heart defects research
- Renal and related cancers
- Pituitary Gland Disorders and Treatments
- Chromosomal and Genetic Variations
- Genetic Syndromes and Imprinting
- Radiation Dose and Imaging
- Pancreatic function and diabetes
- Adipose Tissue and Metabolism
- Chronic Myeloid Leukemia Treatments
- Cancer Diagnosis and Treatment
- Regulation of Appetite and Obesity
- Adrenal and Paraganglionic Tumors
- Genetics and Neurodevelopmental Disorders
- FOXO transcription factor regulation
- Chronic Lymphocytic Leukemia Research
- Genomics and Rare Diseases
Assistance Publique – Hôpitaux de Paris
2016-2025
Université Paris-Saclay
2017-2025
Inserm
2008-2025
Hôpital Antoine-Béclère
2015-2024
CEA Paris-Saclay - Etablissement de Fontenay-aux-roses
2008-2022
Stabilité Génétique, Cellules Souches et Radiations
2022
Direction de la Recherche Fondamentale
2022
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2008-2022
Institut de Radiobiologie Cellulaire et Moléculaire
2022
Université Paris-Sud
2011-2019
Comparative studies suggest that stem cells committed to a cardiac lineage are more effective for improving heart function than those featuring an extra-cardiac phenotype. We have therefore developed population of human embryonic cell (ESC)-derived progenitor cells. Undifferentiated ESCs (I6 line) were amplified and cardiac-committed by exposure bone morphogenetic protein-2 fibroblast growth factor receptor inhibitor. Cells responding these cardio-instructive cues express the transcription...
In mammals, adiponectin and its receptors (AdipoR1 AdipoR2) mRNAs are expressed in various tissues. However, the cellular expression role of system have never been investigated rat ovary. Here, we report presence adiponectin, AdipoR1 AdipoR2 ovaries, granulosa cells. Using RT-PCR western blot, show that proteins for found ovaries. Immunohistochemistry localized theca-interstitial T-I cells, corpus luteum, oocyte less abundantly KGN human cell line, mRNA protein were undetectable; was weakly...
In mammals, IGFs are important for the proliferation and steroidogenesis of ovarian cells. Metformin is an insulin sensitizer molecule used treatment infertility women with polycystic ovary syndrome. It is, however, unclear whether metformin acts on Adenosine 5′ monophosphate-activated protein kinase (AMPK) involved in action various cell types. We investigated effects bovine granulosa response to IGF1 FSH, studied AMPK ovaries. cells from small follicles, (10 mM) reduced production both...
There is now compelling evidence that cells committed to a cardiac lineage are most effective for improving the function of infarcted hearts. This has been confirmed by our pre-clinical studies entailing transplantation human embryonic stem cell (hESC)-derived progenitors in rat and non-human primate models myocardial infarction. These data have paved way translational programme aimed at phase I clinical trial. The main steps this included (i) expansion clone pluripotent hESC generate master...
BACKGROUNDKallmann syndrome (KS) is a genetic disorder associating pubertal failure with congenitally absent or impaired sense of smell. KS related to defective neuronal development affecting both the migration olfactory nerve endings and GnRH neurons. The discovery several mutations responsible for led identification signaling pathways involved in these processes, but so far identified account only 30% cases KS. Here, we attempted identify new genes by using pan-genomic approach.
The AMP-activated protein kinase (AMPK) is a major regulator of energy metabolism involved in fatty acid and cholesterol synthesis. In the ovary, plays key role steroid production. We report presence AMPK rat ovaries, we have investigated its granulosa cells. show using RT-PCR Western blot that mRNAs for alpha1/2 beta1/2 subunits proteins are found ovaries. Immunohistochemistry localized alpha1 subunit cells, corpus luteum, oocyte less abundantly theca Treatment with 1 mm...
Abstract Importance A paradoxical increase of growth hormone (GH) following oral glucose load has been described in ∼30% patients with acromegaly and related to the ectopic expression glucose-dependent insulinotropic polypeptide (GIP) receptor (GIPR) somatotropinomas. Recently, we identified germline pathogenic variants somatic loss heterozygosity lysine demethylase 1A (KDM1A) GIP-dependent primary bilateral macronodular adrenal hyperplasia Cushing's syndrome. The GIPR both pituitary lesions...
Metformin is an anti-diabetic drug commonly used to treat cycle disorders and anovulation in women with polycystic ovary syndrome. However, the effects molecular mechanism of metformin are not entirely understood. We investigated this on steroidogenesis proliferation rat granulosa cells. (10 mM) treatment for 48 h reduced progesterone estradiol (E2) production both basal conditions under FSH stimulation. It also decreased levels HSD3B, CYP11A1, STAR, CYP19A1 proteins response (10(-8) M)...
The 5'AMP-activated protein kinase (AMPK) activation is involved in the meiotic maturation of oocytes ovaries mice and pigs. However, its effects on oocyte appear to be species-specific. We investigated patterns AMPK mitogen-activated kinases (MAPK3/1) phosphorylation during bovine vitro (IVM) metformin, an activator, cumulus-oocyte complexes (COCs) denuded (DOs). In COCs, PRKAA Thr172 decreased, whereas MAPK3/1 increased both cumulus cells IVM. Metformin (5 10 mM) arrested at GV stage COCs...
Although its mechanism of action is still unclear, metformin an anti-diabetic drug effective to restore cyclicity and spontaneous ovulation in women with polycystic ovary syndrome. It may also reduce the risk cancer. We have recently shown that treatment decreases steroidogenesis through AMP-activated kinase (AMPK) granulosa cells various species. Here, we investigated effects molecular mechanisms IGF1-induced proliferation protein synthesis cultured bovine cells. Treatment (10 mM) for 24 h...
Metformin is an insulin sensitizer molecule used for the treatment of infertility in women with polycystic ovary syndrome and resistance. It modulates reproductive axis, affecting release gonadotropin-releasing hormone (GnRH) luteinizing (LH). However, metformin's mechanism action pituitary gonadotropin-secreting cells remains unclear. Adenosine 5′ monophosphate-activated protein kinase (PRKA) involved metformin various cell types. Here, we investigated effects on gonadotropin secretion...
Abstract Purpose The molecular pathogenesis of growth hormone-secreting pituitary adenomas is not fully understood. Cytogenetic alterations might serve as alternative driver events in GNAS mutation–negative somatotroph tumors. Experimental Design We performed cytogenetic profiling obtained from 39 patients with acromegaly and four sporadic gigantism by using array comparative genomic hybridization analysis. explored intratumor DNA copy-number heterogeneity two tumor samples fluorescence situ...
AMP-activated protein kinase (AMPK) is a fuel sensor in glucose, lipid, and cholesterol metabolism. Using RT-PCR Western blot, AMPK subunits mRNAs (alpha1/2, beta1/2, gamma1/2) proteins (alpha1/2 beta1/2) can be found the hen preovulatory follicles precisely both granulosa theca cells. These are organized hierarchy according to their size (F5/6 F1). The smallest number (F1) corresponds largest latest mature stage. Phosphorylation of AMPKalpha on Thr172 acetyl-CoA carboxylase Ser79 higher F4...
Hypothalamic AMP-activated kinase (AMPK) is a key regulator of food intake in mammals. Its role reproduction at the central level and, more precisely, gonadotrophin-releasing hormone (GnRH) release has never been investigated. We showed that each subunit AMPK present immortalised GnRH neurones (GT1-7 cells). Treatment with 5-aminoimidazole-4-carboxamide-1-beta-D-ribonucleoside (AICAR) and metformin, two activators AMPK, increased dose-dependent time-dependent phosphorylation AMPKalpha...
GIP-dependent Cushing's syndrome is caused by ectopic expression of glucose-dependent insulinotropic polypeptide receptor (GIPR) in cortisol-producing adrenal adenomas or bilateral macronodular hyperplasias. Molecular mechanisms leading to GIPR tissue are not known. Here we performed molecular analyses on adrenocortical and hyperplasias obtained from 14 patients with one patient aldosteronism. all adenoma hyperplasia samples occurred through transcriptional activation a single allele the...
Differences of sex development (DSD) is a group rare congenital conditions defined by chromosomal, gonadal and/or phenotypic discordance or atypical sex. The mini-puberty, corresponding to the transient postnatal activation hypothalamic-pituitary-gonadal axis, an important diagnosis window in clinical workup infants with DSD. First objective compare data as well hormone levels during minipuberty between patients and without genetic diagnose. Secondary objectives were assess positive...
ABSTRACT Background Hereditary transthyretin amyloidosis (ATTRv) is a rare genetic disorder caused by mutations in the TTR gene. Associated with various clinical phenotypes like polyneuropathy and cardiomyopathy, ATTRv has historically had poor outcomes. Recent advances biotherapies have significantly improved these This study aimed to assess evolution variant screening since advent of France 2018. Methods nationwide retrospective analyzed data results from patients who underwent gene...
Abstract Background Reproductive dysfunction in the diabetic female rat is associated with altered folliculogenesis and steroidogenesis. However, molecular mechanisms involved reduction of steroid production have not been described. Adiponectin an adipocytokine that has insulin-sensitizing actions including stimulation glucose uptake muscle suppression liver. acts via two receptor isoforms – AdipoR1 AdipoR2 are regulated by hyperglycaemia hyperinsulinaemia liver muscle. We recently...