This report describes the development of a specific and sensitive assay for inhibin B its application to measurement concentrations in plasma during human menstrual cycle. A monoclonal antibody raised against synthetic peptide from betaB-subunit was combined with an alpha-subunit sequence double enzyme-linked immunosorbent format. The validated had limit detection 10 pg/mL 0.5% cross-reactivity A. Using this immunoassay, we found that concentration rose rapidly early follicular phase peak...

Seventeen women and four men with galactorrhoea associated hypogonadism have been treated bromocriptine for 2 to 28 months. In 18 patients the gonadal status became normal as improved. The gonadally unresponsive had either pituitary tumours or premature menopause. Prolactin levels fell treatment; withdrawal of drug was an increase in serum prolactin a recurrence hypogonadism. Two tried become pregnant on treatment both succeeded. Raised appear block actions gonadotrophins at level rather...

We used a recently developed ELISA format to test the hypothesis that inhibin B is physiologically active form of in men. measured and compared A, B, pro-alpha-C-related immunoreactive peptides (pro-alpha-C-RI) normal men before after perturbations their gonadotropin levels baseline values with various disturbances hypothalamic-pituitary-testicular axis including idiopathic hypogonadotropic hypogonadism, infertile elevated FSH, Klinefelter's syndrome, orchidectomized Mean serum...

Summary OBJECTIVE We have developed and validated a two‐site immunoassay for the measurement of dimeric inhibin in plasma subsequently measured levels through normal female menstrual cycle. DESIGN Recombinant added to samples was quantitatively recovered both follicular luteal phase, serial dilutions were tested parallelism similar recombinant 32kDa inhibin. Daily assayed from four women PATIENTS (a) Four groups six who (i) phase cycle, (ii) (iii) post‐menopausal (iV) had received hMG induce...

SUMMARY The pulsatile secretion of gonadotrophins and ovarian steroids was studied in normal women at different stages the menstrual cycle. concentration LH, FSH, Prolactin (PRL), oestradiol progesterone were measured samples plasma collected every 15 min for 6 h frequency amplitude each episodic pulse hormone estimated. Although significant fluctuations occurred hormone, LH showed most easily identifiable pulses which increased significantly from early follicular to late phase cycle...

This study sought to compare circulating and follicular fluid (FF) concentrations of dimeric inhibin A B utilizing specific two-site ELISAs for these hormones in normal older younger ovulatory women. METHODS. Normally ovulating women age 40-45 (n = 10) 20-25 13) were studied throughout the phase with daily blood sampling, transvaginal ultrasound examinations, dominant follicle aspiration. When reached a mean diameter 16 mm or serum estradiol (E2) was > 550 pmol/L, 10,000 IU hCG administered...

Inhibin is a glycoprotein hormone that defined on the basis of inhibition pituitary FSH production, However, previous data have not shown any correlation between RIA measurements inhibin and in men. New enzyme-linked immunosorbent assays, specific for A, B, pro-alphaC-related immunoreactivity, were applied to measurement 32 healthy Further B pro-alphaC-RI carried out groups men exhibiting wide range concentrations, including semen donors, infertile men, with elevated concentrations. A was...

The concentrations of FSH, LH, prolactin, oestradiol and progesterone were measured in peripheral plasma follicular fluid women throughout the menstrual cycle. With exception pituitary steroid hormones correlated with those plasma. Follicle-stimulating hormone was present a greater number small follicles (smaller than 8mm) during or just after peaks FSH During mid-follicular phase concentration both from large (larger equal to 8 mm) high. late contained high amounts addition oestradiol, low...

The oxytocin and prolactin responses to suckling were measured in 10 women early (n = 5) established lactation 5). Oxytocin was released a pulsatile manner during all women, but the response not related milk volume, response, or parity of mother. In plasma concentrations increased three minutes before began. five this occurred baby crying, it coincided with becoming restless expectation feed, while two corresponded mother preparing for feed. There no stimuli other than stimulation nipple...

Subcutaneous self-administration of synthetic gonadotrophin-releasing hormone 500 μg eight-hourly for up to one year by 12 male patients (five prepubertal) with clinical hypogonadism due hypothalamic or pituitary disease resulted in the synthesis and continued release luteinizing (LH) follicle-stimulating (FSH). There was a rise circulating androgen levels all patients. Improvements pubertal ratings were seen some prepubertal Potency returned adults spermatogenesis induced maintained four...

An adequate inter-birth interval is of great importance because it enables a mother to recover her physical and emotional strength between pregnancies confers upon the child advantages better health development (Morley, 1977). Breast feeding naturally evolved method ensuring an but contraceptive effect breast has been largely dismissed in developed countries on grounds that unreliable family planning for individual mothers (Kamal et al., 1969). The use artificial contraception varies very...

The control of fecundity is critical in determining mammalian offspring survival. It regulated principally by the ovulation rate, so that primates and large farm species commonly have a single offspring. Previously, several mutations been identified sheep which increase naturally low rate; although some cases homozygous ewes are infertile. In present study we detailed characterization novel mutation growth differentiation factor 9 (GDF9), found Icelandic Thoka sheep. This base change...

The ovarian secretion of estradiol, androstenedione, testosterone, and progesterone the concentration LH, FSH, prolactin were measured in periovulatory period five ewes with or utero-ovarian autotransplants. Samples jugular venous blood collected every 1 2 h before for 96 after induction luteal regression on Day 10 cycle by injection 100 µg cloprostenol. Progesterone vein plasma fell to less than ng/ml all 24 h, coincidental this decline there was a significant increase LH an associated rise...

Patterns of infant feeding, basal prolactin concentrations, and ovarian activity were studied longitudinally in 27 breast-feeding mothers from delivery until first ovulation. Suckling frequency (6.1 feeds/day) suckling duration (122 mins/day) reached peak values four weeks post partum remained relatively constant the introduction supplementary food at a mean 16 partum. There subsequently sharp declines both suckling, which correlated closely with concentrations. None ovulated during...

SUMMARY A method is described for the extraction and concentration of oxytocin from plasma which simpler more rapid than other available procedures. It has proved satisfactory both radioimmunoassay bioassay circulating oxytocin. The recovery added was 60 ± 5·5% showed no significant variation between pregnant non-pregnant women, or species. sensitivity assay related to volume extracted. With a 10 ml sample previously, maximum under optimal conditions 0·75 μu. (1·5pg)/ml. In second stage...

Highlights•A circannual timer may reside in the pituitary pars tuberalis thyrotroph•This is defined by a digital switching mechanism controlling EYA3 expression•The clockwork drives morphogenic cycle PT and hypothalamus•This involves recapitulation of developmental programSummaryPersistent free-running (approximately year-long) rhythms have evolved animals to regulate hormone cycles, drive metabolic (including hibernation), time annual reproduction. Recent studies photoperiodic input this...

Several different miRNAs have been proposed to regulate ovarian follicle function; however, very limited information exists on the spatiotemporal patterns of miRNA expression during development. The objective this study was identify, using microarray, profiles associated with growth and regression dominant-size follicles in bovine monovular ovary characterize their distribution were collected from abattoir ovaries classified as small (4-8 mm) or large (12-17 mm); latter further healthy...

A comparison of serum inhibin levels in men and women was undertaken using a sensitive sheep pituitary cell vitro bioassay newly developed heterologous RIA. The RIA based on an antiserum raised to bovine 31K [125I]31K as tracer. Bovine α- β-subunits, activin-A, transforming growth factor-β, Mullerian inhibitory substance did not cross-react the In both assays, dilutions gave response lines parallel that partially purified human follicular fluid preparation used standard. Negligible bio...