A5079128102- Hepatitis C virus research
- Hepatitis B Virus Studies
- Liver Disease Diagnosis and Treatment
- Systemic Lupus Erythematosus Research
- Bone health and treatments
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- Growth Hormone and Insulin-like Growth Factors
- Bone Metabolism and Diseases
- Bone and Dental Protein Studies
- Hepatitis Viruses Studies and Epidemiology
- Cancer Immunotherapy and Biomarkers
- Fibroblast Growth Factor Research
- RNA Interference and Gene Delivery
- Bone health and osteoporosis research
- Bone Tissue Engineering Materials
- Apelin-related biomedical research
- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- Lipid Membrane Structure and Behavior
- Axon Guidance and Neuronal Signaling
- Advanced Cellulose Research Studies
- High Altitude and Hypoxia
- Digestive system and related health
Centre National de la Recherche Scientifique
1998-2025
Université Grenoble Alpes
2017-2025
Inserm
1998-2025
Centre Hospitalier Universitaire de Grenoble
2025
Institut polytechnique de Grenoble
2017-2023
University Hospital of Geneva
2014-2022
Institut de Biosciences et Biotechnologies
2021-2022
Université Bourgogne Franche-Comté
2022
Université de Bourgogne
2017-2022
Université de Picardie Jules Verne
2010-2017
In vitro infection of adult normal human hepatocytes in primary culture has been performed for investigating the replication cycle hepatitis C virus (HCV) differentiated cells. Hepatocytes were prepared from liver tissue resected donors who tested negative HCV, and inoculation was 3 days after plating with 33 HCV serum samples different load genotype. The presence intracellular RNA, detected by a strand-specific rTth RT-PCR assay, used as evidence infection. A kinetics analysis revealed that...
Thin cellulose nanofiber (CNF) nanostructured substrates with varying roughness, stiffness (Young's modulus), porosity, and swelling properties were produced by the conditions used during fabrication. It was shown that increased heat exposure, CNF substrate porosity in an aqueous state decreased while Young's modulus a water submerged increased. In this study, adhesion viability of mesenchymal stem cells (MSCs) cultured on will be presented. Viability D1/BALBc MSCs assessed for 24 48 h, it...
Abstract Background and Aims Netrin‐1 displays protumoral properties, though the pathological contexts processes involved in its induction remain understudied. The liver is a major model of inflammation‐associated cancer development, leading to HCC. Approach Results A panel cell biology biochemistry approaches (reverse transcription quantitative polymerase chain reaction, reporter assays, run‐on, polysome fractionation, cross linking immunoprecipitation, filter binding assay, subcellular...
Bone sialoprotein (BSP) belongs to the small integrin-binding ligand, N-linked glycoprotein (SIBLING) family, whose members play multiple and distinct roles in development, turnover, mineralization of bone dentin. The functions BSP remodeling are not yet well established. We previously showed that knockout (BSP−/−) mice exhibit a higher trabecular volume, concomitant with lower remodeling, than wild-type (BSP+/+) mice. To determine whether turnover can be stimulated absence BSP, we subjected...
After a Christmas party in restaurant, 48 (68%) of the 82 guests contracted calicivirus gastroenteritis. The epidemiological investigation showed that salad was strongly associated with disease episode (RR = 2.43, P 0.0005). Similar symptoms occurred among other customers who had meal at same restaurant on evening. A foodhandler only prepared and appetizers became sick about 30 min after end his shift. He been free while preparing food. Few outbreak investigations have shown transmission by...
Abstract Background Pegylated interferon alpha 2 (a or b) plus ribavirin is the most effective treatment of chronic hepatitis C but a large proportion patients do not respond to therapy. So, it interesting improve efficacy. Interferon type I composed 12 different subtypes. Each subtype signals by Jak-Stat pathway modulations in antiviral activity was previously described. Methods Using virus (HCV) culture system, we have tested anti-HCV each We analyzed effect on HCV multiplication and...
Significant progress has been made in Hepatitis C virus (HCV) culture since the JFH1 strain cloning. However, developing efficient and physiologically relevant systems for all viral genotypes remains an important goal. In this work, we aimed at producing a high titer derived to test different hepatic cells' permissivity. To end, performed successive infections obtained reaching titers. Six potential adaptive mutations were identified (I599V E2, R1373Q M1611T NS3, S2364P C2441S NS5A R2523K...
Introduction: Upon BMP-2 stimulation, the osteoblastic lineage commitment in C2C12 myoblasts is associated with a microenvironmental change that occurs over several days. How does operate switch adhesive machinery to adapt new microenvironment and drive bone cell fate not well understood. Here, we addressed this question for delivered either solution or physically bound of biomimetic film, mimic its presentation cells via extracellular matrix (ECM). Methods: Biommetics films were prepared...
Abstract Background/Aim The role of humoral immunity in hepatitis C virus (HCV) infection is poorly understood. Nevertheless, there increasing interest characterizing the neutralizing antibodies serum HCV-infected patients. Focus reduction assays have been widely used to evaluate antibody responses against a range non-cytopathic viruses. Based on recent development HCV cell culture system using genotype 2 JFH-1-strain, we developed focus assay for HCV-neutralizing antibodies. Methods was...
Peak bone mass acquisition is influenced by environmental factors including dietary intake. A low-protein diet delays body and skeletal growth in association with a reduction serum IGF-1 whereas FGF21 increased selective amino acid deprivation. Calcium (Ca) phosphorous (P) are also key nutrients for health, inadequate intakes reduce accrual calciotropic hormone modulation. Besides, the effect of calcium supplementation on prepubertal children appears to be protein To further explore...
Nonviral systems, such as lipid nanoparticles, have emerged reliable methods to enable nucleic acid intracellular delivery. The use of cationic lipids in various formulations nanoparticles enables the formation complexes with cargo and facilitates their uptake by target cells. However, due small size highly charged nature, these nanocarrier systems can interact vivo antigen-presenting cells (APCs), dendritic (DCs) macrophages. As this might prove be a safety concern for developing therapies...
Obstructive sleep apnea (OSA) syndrome is characterized by chronic intermittent hypoxia and associated with an increased risk of all-cause mortality, including cancer mortality. Hepatocellular carcinoma (HCC) the most common type liver cancer, increasing incidence high However, link between HCC OSA-related remains unclear. Herein, we used a diethylnitrosamine (DEN)-induced model to investigate whether has impact on progression. To elucidate mechanisms, first evaluated status in DEN-induced...
The development of different cell culture models has greatly contributed to increased understanding the hepatitis C virus (HCV) life cycle. However, it is still challenging grow HCV clinical isolates in culture. If overcome, this would open new perspectives study biology, including drug‐resistant variants emerging with antiviral therapies. In we hypothesized that hurdle could be due presence inhibitory factors patient serum. Combining polyethylene glycol precipitation, iodixanol gradient,...
A trans-packaging system for hepatitis C virus (HCV) replicons lacking envelope glycoproteins was developed. The were efficiently encapsidated into infectious particles after expression in trans of homologous HCV proteins under the control an adenoviral vector. Interestingly, core or core, p7 and NS2 with did not enhance trans-encapsidation. Expression heterologous proteins, presence absence NS2, rescue single-round particle production. To increase titre homologous, our system, successive...
Carbohydrate binding agents (CBAs), including natural lectins, are more and considered as broad-spectrum antivirals. These molecules able to directly inhibit many viruses such Human Immunodeficiency Virus (HIV), Hepatitis C (HCV), Dengue Virus, Ebola or Severe Acute Respiratory Syndrome Coronavirus through envelope protein N-glycans. In the case of HIV, it has been shown that CBAs select for mutant with N-glycosylation site deletions which sensitive neutralizing antibodies. this study we...