A5081906227- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- TGF-β signaling in diseases
- Bone Tissue Engineering Materials
- Cell Adhesion Molecules Research
- Bone Metabolism and Diseases
- Connective Tissue Growth Factor Research
- Periodontal Regeneration and Treatments
- Electrospun Nanofibers in Biomedical Applications
- Axon Guidance and Neuronal Signaling
- Connective tissue disorders research
- Bone health and treatments
- Biochemical and Structural Characterization
- Proteoglycans and glycosaminoglycans research
- Planarian Biology and Electrostimulation
- Inflammatory mediators and NSAID effects
- Pulmonary Hypertension Research and Treatments
- Skin and Cellular Biology Research
- Nanofabrication and Lithography Techniques
Université Grenoble Alpes
2020-2024
CEA Grenoble
2020-2024
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2020-2024
Centre National de la Recherche Scientifique
2020-2024
Inserm
2022-2024
Biomécanique et Bioingénierie
2023
Institut polytechnique de Grenoble
2019-2022
Laboratoire des Matériaux et du Génie Physique
2019-2022
Max Planck Institute for Medical Research
2017-2019
Max Planck Institute for Intelligent Systems
2014-2018
A wide variety of cell types exhibit substrate topography-based behavior, also known as contact guidance. However, the precise cellular mechanisms underlying this process are still unknown. In study, we investigated guidance by studying reaction human endothelial cells (ECs) to well-defined microgroove topographies, both during and after initial spreading. As cytoskeleton plays a major role in adaptation topographical features, two methods were used perturb cytoskeletal structures....
Abstract The interactions between a cancer cell and its extracellular matrix (ECM) have been the focus of an increasing amount investigation. role intermediate filament keratin in has also coming into late, but more research is needed to understand how this piece fits puzzle cytoskeleton-mediated invasion metastasis. In Panc-1 invasive pancreatic cells, phosphorylation conjunction with actin inhibition was found be sufficient reduce area below either treatment alone. We then analyzed...
Although integrins are responsible for the interaction of cells with their environment, e.g., extracellular matrix or artificial substrates, there is still a lack knowledge about role in cell adhesion and migration on protein-coated substrates microtopography. Understanding such interactions could lead to new applications medical implants as well shed light processes embryonic development, angiogenesis, wound healing, tumor progression. In this work, influence surface topography chemistry...
Introduction: Upon BMP-2 stimulation, the osteoblastic lineage commitment in C2C12 myoblasts is associated with a microenvironmental change that occurs over several days. How does operate switch adhesive machinery to adapt new microenvironment and drive bone cell fate not well understood. Here, we addressed this question for delivered either solution or physically bound of biomimetic film, mimic its presentation cells via extracellular matrix (ECM). Methods: Biommetics films were prepared...
The spatial organization of cell-surface receptors is fundamental for the coordination biological responses to physical and biochemical cues extracellular matrix. How serine/threonine kinase receptors, ALK3-BMPRII, cooperate with integrins upon BMP2 drive cell migration unknown. Whether dynamics between BMP intertwine in space time guide adhesive processes yet be elucidated. We found that stimulation controls BMPRs by segregating ALK3 from BMPRII into β3 integrin-containing focal adhesions....
In vivo, cells encounter different physical and chemical signals in the extracellular matrix (ECM) which regulate their behavior. Examples of these are micro- nanometer-sized features, rigidity, composition ECM. The study cell responses to such cues is important understand complex functions, some diseases, basis for development new biomaterials applications medical implants or regenerative medicine. Therefore, methods surface modifications with controlled features crucial. this work, we...
Bone morphogenetic proteins (BMPs) and transforming growth factors (TGF-β) are members of the TGF-β superfamily, known for their roles in several physiological pathological processes. These to bind vivo BMP receptors, respectively, which induces phosphorylation Smad (pSmad) transcription factors. This pathway is generally studied with Western blot luciferase bioluminescence assay, presents some limitations.
ABSTRACT Whereas soft biomaterial is not able to induce cell spreading, BMP-2 presented by a film has been described be sufficient trigger migration and downstream signaling. Based on thin polyelectrolyte films of controlled stiffness, we investigated whether the presentation four BMP members (2, 4, 7, 9) in matrix-bound manner may differentially impact adhesion bone differentiation skeletal progenitors. We performed high content automated screening cellular responses, including number,...
Abstract Bone morphogenetic proteins (BMPs) and transforming growth factors (TGF-β) are members of the superfamily, known for their role in several physiological pathological processes. These to bind vivo BMP TGF-β receptors respectively, which induces phosphorylation Smad (pSmad) transcription factors. This pathway is generally studied with western blot luciferase bioluminescence assay, present some limitations. In our work, we developed optimized a high-content immunofluorescence assay...