A5005521371- Bone Tissue Engineering Materials
- TGF-β signaling in diseases
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Cellular Mechanics and Interactions
- Immunotherapy and Immune Responses
- Periodontal Regeneration and Treatments
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Nitric Oxide and Endothelin Effects
- Polymer Surface Interaction Studies
- 3D Printing in Biomedical Research
- Connective Tissue Growth Factor Research
- Cell Adhesion Molecules Research
- Mitochondrial Function and Pathology
- Orthopaedic implants and arthroplasty
- Proteoglycans and glycosaminoglycans research
- Inflammatory mediators and NSAID effects
- Metabolism and Genetic Disorders
- Connective tissue disorders research
- Cell Image Analysis Techniques
- Adipose Tissue and Metabolism
- Muscle metabolism and nutrition
- Pulmonary Hypertension Research and Treatments
- Bone Metabolism and Diseases
- Electrospun Nanofibers in Biomedical Applications
Centre National de la Recherche Scientifique
2014-2024
Université Grenoble Alpes
2009-2024
CEA Grenoble
2009-2024
Inserm
2022-2024
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2009-2024
Institut polytechnique de Grenoble
2018-2023
Biomécanique et Bioingénierie
2023
Laboratoire des Matériaux et du Génie Physique
2018-2022
Institut Néel
2018-2022
Institut de Biologie Structurale
2009-2021
Protective T cell responses elicited along chronic human CMV (HCMV) infections are sometimes dominated by CD8 clones bearing highly related or identical public TCR in unrelated individuals. To understand the principles that guide emergence of these responses, we have performed structural, biophysical, and functional analyses an immunodominant (RA14) directed against a major HLA-A*0201-restricted HCMV Ag (pp65(495-503)) selected vivo from diverse repertoire after stimulations. Unlike two TCRs...
Abstract Heterozygous activin receptor-like kinase 1 ( ALK1 ) mutations are associated with two vascular diseases: hereditary hemorrhagic telangiectasia (HHT) and more rarely pulmonary arterial hypertension (PAH). Here, we aimed to understand the impact of on BMP9 BMP10 transcriptomic responses in endothelial cells. Endothelial colony-forming cells (ECFCs) microvascular (HMVECs) carrying loss function were isolated from newborn HHT adult PAH donors, respectively. RNA-sequencing was performed...
The reconstruction of large bone defects (12 cm3) remains a challenge for clinicians. We developed new critical-size mandibular defect model on minipig, close to human clinical issues. analyzed the obtained by 3D-printed scaffold made clinical-grade polylactic acid (PLA), coated with polyelectrolyte film delivering an osteogenic bioactive molecule (BMP-2). compared results (computed tomography scans, microcomputed histology) gold standard solution, autograft. demonstrated that dose BMP-2...
The reconstruction of critical-size bone defects in long bones remains a challenge for clinicians. A new osteoinductive medical device is developed here repair by combining 3D-printed architectured cylindrical scaffold made clinical-grade polylactic acid (PLA) with polyelectrolyte film coating delivering the osteogenic morphogenetic protein 2 (BMP-2). This film-coated used to sheep metatarsal 25-mm defect. In vitro and vivo biocompatibility PLA material proved according ISO standards....
Measuring neurite length is crucial in neurobiology because it provides valuable insights into the growth, development, and function of neurons. In particular, fundamental to study neuronal development differentiation, neurons responses drugs, neurodegenerative diseases plasticity. Surprisingly, there currently a lack tools for high content analysis. this article, we present CABaNe, as an open source, content, rule based Image J macro cell analysis, including their length. We explain main...
An automatic method is established for layer-by-layer (LbL) assembly of biomimetic coatings in cell culture microplates using a commercial liquid-handling robot. Highly homogeneous thin films are formed at the bottom each microwell. The LbL film-coated compatible with common cellular assays, microplate readers and automated microscopes. Cellular adhesion screened on crosslinked peptide-functionalized stem differentiation response to increasing doses bone morphogenetic proteins (2, 4, 7, 9)....
The role of glycosaminoglycans (GAGs) in modulating bone morphogenetic protein (BMP) signaling represents a recent and underexplored area. Conflicting reports suggest dual effect: some indicate positive influence, while others demonstrate negative impact. This duality suggests that the localization GAGs (either at cell surface or within extracellular matrix) specific type GAG may dictate their role. precise sulfation patterns heparan sulfate (HS) responsible for BMP2 binding remain elusive....
Human adipose-derived stromal cells (hASCs) have been gaining increasing popularity in regenerative medicine thanks to their multipotency, ease of collection, and efficient culture. Similarly other cells, function is particularly sensitive the culture conditions, including composition medium. Given large number parameters that can play a role specification, rapid assessment would be beneficial allow optimization parameters. Herein we used design experiments (DOE) method rapidly screen...
The structural rules governing peptide/MHC (pMHC) recognition by T cells remain unclear. To address this question, we performed a characterization of several HLA-A2/peptide complexes and assessed in parallel their antigenicity, analyzing the frequency corresponding Ag-specific naive A2(+) A2(-) individuals, as well within CD4(+) CD8(+) subsets. We were able to find correlation between specific cell peptide solvent accessibility and/or mobility for subset moderately prominent peptides....
Two-photon polymerization has recently emerged as a promising technique to fabricate scaffolds for three-dimensional (3D) cell culture and tissue engineering. Here, we combined 3D-printed microscale fabricated using two-photon with bioactive layer-by-layer film coating. This coating consists of hyaluronic acid poly(l-lysine) controlled stiffness, loaded fibronectin bone morphogenic proteins 2 4 (BMP2 BMP4) matrix-bound proteins. Planar films were prepared liquid handling robot directly in...
Introduction: Upon BMP-2 stimulation, the osteoblastic lineage commitment in C2C12 myoblasts is associated with a microenvironmental change that occurs over several days. How does operate switch adhesive machinery to adapt new microenvironment and drive bone cell fate not well understood. Here, we addressed this question for delivered either solution or physically bound of biomimetic film, mimic its presentation cells via extracellular matrix (ECM). Methods: Biommetics films were prepared...
The automation of liquid-handling routines offers great potential for fast, reproducible, and labor-reduced biomaterial fabrication but also requires the development special protocols. Competitive systems demand a high degree in miniaturization parallelization while maintaining flexibility regarding experimental design. Today, there are only few possibilities automated biomaterials inside multiwell plates. We have previously demonstrated that streptavidin-based biomimetic platforms can be...
The mitochondrial ADP/ATP carrier (AAC) is a prominent actor in the energetic regulation of cell, importing ADP into mitochondria and exporting ATP toward cytoplasm. Severe genetic diseases have been ascribed to specific mutations this membrane protein. How minute, well-localized modifications transporter impact function remains, however, largely unclear. Here, for first time, relationship between all documented pathological AAC its transport properties established. Activity measurements...
The NADPH oxidase is the sole enzymatic complex that produces, in a controlled way, superoxide anions. In phagocytes, it constituted by assembly of four cytosolic (p67(phox) , p47(phox) p40(phox) and Rac) two membrane (p22(phox) Nox2) proteins. response to pro-inflammatory mediators, activated. cells, arachidonic acid (cis-AA), released activated phospholipase A2, also plays role activation complex, but mechanism action cis-AA still matter for debate. cell-free systems, commonly used...
Bone morphogenetic proteins (BMPs) are an important family of growth factors playing a role in large number physiological and pathological processes, including bone homeostasis, tissue regeneration, cancers. In vivo, BMPs bind successively to both BMP receptors (BMPRs) type I II, promiscuity has been reported. this study, we used biolayer interferometry perform parallel real-time biosensing deduce the kinetic parameters (ka, kd) equilibrium constant (KD) for range BMP/BMPR combinations...
Abstract the reconstruction of large bone defects (12 cm 3 ) remains a challenge for clinicians. We developed new critical-size mandibular defect model on mini-pig, close to human clinical issues. analyzed obtained by 3D printed scaffold made clinical-grade PLA, coated with polyelectrolyte film delivering an osteogenic bioactive molecule (BMP-2). compared results (CT-scan, μCT, histology) gold standard solution, autograft. demonstrated that dose BMP-2 delivered from significantly influenced...
The most severe form of human malaria is caused by the parasite Plasmodium falciparum. Despite current need, there no effective vaccine and parasites are becoming resistant to antimalarials available. Therefore, an urgent need discover new drugs from targets that have not yet suffered drug pressure with aim overcoming problem emerging resistance. Membrane transporters, such as P. falciparum Ca(2+)-ATPase 6 (PfATP6), sarcoplasmic/endoplasmic reticulum (SERCA), been proposed potentially good...
The spatial organization of cell-surface receptors is fundamental for the coordination biological responses to physical and biochemical cues extracellular matrix. How serine/threonine kinase receptors, ALK3-BMPRII, cooperate with integrins upon BMP2 drive cell migration unknown. Whether dynamics between BMP intertwine in space time guide adhesive processes yet be elucidated. We found that stimulation controls BMPRs by segregating ALK3 from BMPRII into β3 integrin-containing focal adhesions....