A5041730450- Nanoparticles: synthesis and applications
- Air Quality and Health Impacts
- Nanoparticle-Based Drug Delivery
- Microplastics and Plastic Pollution
- Inhalation and Respiratory Drug Delivery
- Graphene and Nanomaterials Applications
- Neonatal Respiratory Health Research
- Fusion materials and technologies
- Radiation Therapy and Dosimetry
- Healthcare and Environmental Waste Management
- Computational Drug Discovery Methods
- Nuclear Materials and Properties
- Nanoplatforms for cancer theranostics
- Carcinogens and Genotoxicity Assessment
- RNA Interference and Gene Delivery
- Alzheimer's disease research and treatments
- Cytokine Signaling Pathways and Interactions
- Advanced Electron Microscopy Techniques and Applications
- Occupational exposure and asthma
- Immune cells in cancer
- Atherosclerosis and Cardiovascular Diseases
- Environmental remediation with nanomaterials
- Advanced biosensing and bioanalysis techniques
- nanoparticles nucleation surface interactions
- Cystic Fibrosis Research Advances
Institut Méditerranéen de Biodiversité et d'Ecologie Marine et Continentale
2015-2021
Aix-Marseille Université
2015-2021
Institut de Recherche pour le Développement
2015-2019
Centre National de la Recherche Scientifique
2015-2019
Université d'Avignon et des Pays de Vaucluse
2019
University of Pisa
2014-2016
Joint Research Centre
2011-2016
Johannes Gutenberg University Mainz
2008-2013
University Medical Center of the Johannes Gutenberg University Mainz
2011-2013
Joint Research Centre
2013
During the last years engineered nanoparticles (NPs) have been extensively used in different technologies and consequently many questions arisen about risk impact on human health following exposure to nanoparticles. Nevertheless, at present knowledge cytotoxicity induced by NPs is still largely incomplete. In this context, we investigated gold (AuNPs), which differed size purification grade (presence or absence of sodium citrate residues particle surface) vitro, alveolar type-II (ATII)-like...
Aim: Targeted biocompatible nanoplatforms presenting multiple therapeutic functions have great potential for the treatment of cancer. Materials & methods: Multifunctional nanocomposites formed by polymeric nanoparticles (PNPs) containing two cytotoxic agents – drug alisertib and silver were synthesized. These PNPs been conjugated with a chlorotoxin, an active targeting 36-amino acid-long peptide that specifically binds to MMP-2, receptor overexpressed brain cancer cells. Results: The...
The use of gold nanoparticles (AuNPs) for diagnostic applications and drug gene-delivery is currently under intensive investigation. For such applications, biocompatibility the absence cytotoxicity AuNPs essential. Although generally considered as highly biocompatible, previous in vitro studies have shown that certain human epithelial cells was observed. In particular, degree purification (presence sodium citrate residues on particles) to affect proliferation induce these cells. To expand...
Abstract Background Cobalt-ferrite nanoparticles (Co-Fe NPs) are attractive for nanotechnology-based therapies. Thus, exploring their effect on viability of seven different cell lines representing organs the human body is highly important. Methods The toxicological effects Co-Fe NPs were studied by in-vitro exposure A549 and NCIH441 cell-lines (lung), precision-cut lung slices from rat, HepG2 cell-line (liver), MDCK (kidney), Caco-2 TC7 (intestine), TK6 (lymphoblasts) primary mouse...
Despite human gastrointestinal exposure to nanoparticles (NPs), data on NPs toxicity in intestinal cells are quite scanty. In this study we evaluated the induced by zinc oxide (ZnO) and titanium dioxide (TiO2) Caco-2 cells. Only ZnO produced significant cytotoxicity, two different assays. The presence of foetal calf serum culture medium significantly reduced as well ion leakage NP-cell interaction. increased intracellular amount reactive oxygen species (ROS) after 6 h treatment. However,...
The wide use of titanium dioxide nanoparticles (TiO2 NPs) in industrial applications requires the investigation their effects on human health. In this context, we investigated nanosized and bulk titania two different crystalline forms (anatase rutile) vitro. By colony forming efficiency assay, a dose-dependent reduction clonogenic activity Balb/3T3 mouse fibroblasts was detected presence rutile, but not case anatase NPs. Similarly, cell transformation assay micronucleus test showed that...
The toxicological effects of cobalt nanoparticles (Co-NPs) aggregates were examined and compared with those ions (Co-ions) using six different cell lines representing lung, liver, kidney, intestine, the immune system. Dose-response curves studied in concentration range 0.05–1.0mM, employing 3-(4,5-dimethylthiazol-2-Yl)-2,5-diphenyltetrazolium bromide test, neutral red, Alamar blue as end point assays following exposures for 48 72 h. Data analysis predictive modeling obtained data sets...
Abstract: Gold nanoparticles (Au NPs) are used in many fields, including biomedical applications; however, no conclusive information on their potential cytotoxicity and genotoxicity mechanisms is available. For this reason, experiments human primary lymphocytes murine macrophages (Raw264.7) were performed exposing cells to spherical citrate-capped Au NPs with two different nominal diameters (5 nm 15 nm). The proliferative activity, mitotic, apoptotic, necrotic markers, as well chromosomal...
The alveolar region of the lung is an important target for drug and gene delivery approaches. Treatment with drugs often necessary under pathophysiological conditions, in which there acute inflammation organ. Therefore, vitro models alveolar-capillary barrier, mimic inflammatory conditions region, would be useful to analyse predict effects novel on healthy or inflamed tissues. epithelial cell line H441 was cultivated primary isolated human pulmonary microvascular endothelial cells (HPMECs)...
An in vitro human alveolar barrier established by the coculture of epithelial cell line NCI-H441 with endothelial ISO-HAS1 was used to evaluate effects amorphous silicon dioxide nanoparticles (SiNPs), presence or absence THP-1 cells (monocytes). SiNPs exposure induced production proinflammatory cytokine and oxidative stress. A high release TNF-α IL-8 epithelial/endothelial cells, potentiated could contribute observed downregulation surfactant proteins mRNA expression resulting damage...
Poorly soluble cobalt (II, III) oxide particles (Co3O4P) are believed to induce in vitro cytotoxic effects via a Trojan-horse mechanism. Once internalized into lysosomal and acidic intracellular compartments, Co3O4P slowly release low amount of ions (Co(2+)) that impair the viability cultures. In this study, we focused on genotoxic potential by performing comprehensive investigation DNA damage exerted BEAS-2B human bronchial epithelial cells.Our results demonstrate poorly enhanced formation...
The FP7 Sanowork project was aimed to minimise occupational hazard and exposure engineered nanomaterials (ENM) through the surface modification in order prevent possible health effects. In this frame, a number of nanoparticles (NP) have been selected, among which zirconium (ZrO 2 ) titanium (TiO dioxide. study, we tested ZrO NP TiO either their pristine (uncoated) form, or modified with citrate and/or silica on surface. As benchmark material, Aeroxide® P25 used. We assessed cytotoxicity,...
The International Thermonuclear Experimental Reactor (ITER) is an international project aimed at the production of carbon-free energy through use thermonuclear fusion. During ITER operation, in case a loss-of-vacuum-accident, tungsten nanoparticles (W-NPs) could potentially be released into environment and induce occupational exposure via inhalation. W-NPs toxicity was evaluated on MucilAir™, 3D vitro cell model human airway epithelium. MucilAir™ exposed for 24 h to metallic ITER-like milled...
Due to their physico-chemical characteristics, gold nanoparticles (AuNPs) seem be suitable for biomedical and therapeutic applications even if conflicting data on toxicological profiles are present in literature. In order better understand AuNPs could safe we must consider different biological endpoints such as cytotoxicity, genotoxicity, inflammation biopersistence. Starting from these considerations, one of the first issues assessed is can internalized by cells. this work, propose a...
Synchrotron radiation (SR) X‐ray microscopy combined with fluorescence (XRF) microspectroscopy provides unique information that have pushed the frontiers of biological research, particularly when investigating intracellular mechanisms. This work reports an SR‐XRF investigation on distribution and potential toxicity Fe 2 O 3 CoFe 4 nanoparticles (NPs) in U87MG glioblastoma‐astrocytoma cells. The cells exposed to NPs concentrations ranging from 5 250 µg/ml for 24 h were analyzed order monitor...
Sulfur mustard (SM) is a strong alkylating agent. Inhalation of SM causes acute lung injury accompanied by severe disruption the airway barrier. In our study, we tested effects after exposure in an vitro coculture bronchial model proximal To achieve this, seeded normal human epithelial explant-outgrowth cells (HBEC) together with fibroblasts as bilayer on filter plates and exposed 31 days differentiation to various concentrations (30, 100, 300, 500μM). The HBEC formed confluent layers,...
During International Thermonuclear Experimental Reactor operation, due to plasma–wall interaction, particles/dust will be created in sizes ranging from nanometers tens of microns. The dust properties, especially their ability covered by a thin oxide electrostatic insulating layer, and surface topology deeply affect tritium inventory. Consequently, physico-chemical properties specific tritiated tungsten particles consequence on particle behavior the facility environment must carefully...
Tungsten was chosen as a wall component to interact with the plasma generated by International Thermonuclear Experimental fusion Reactor (ITER). Nevertheless, during operation tritiated tungsten nanoparticles (W-NPs) will be formed and potentially released into environment following Loss-Of-Vacuum-Accident, causing occupational or accidental exposure. We therefore investigated, in bronchial human-derived BEAS-2B cell line, cytotoxic epigenotoxic effects of two types ITER-like W-NPs (plasma...
Abstract L‐selectin has been suggested to play a role in the pathogenesis of experimental autoimmune encephalomyelitis (EAE), an animal model multiple sclerosis. Here we demonstrate that –/– SJL mice are susceptible proteolipid protein (PLP)‐induced EAE because compromised antigen‐specific T cell proliferation peripheral lymph nodes is fully compensated by response raised their spleen. Transfer PLP‐specific cells into syngeneic recipients induced independent presence or absence on recipient....
Immiscible polymer blends tend to undergo phase separation with the formation of nanoscale architecture which can be used in a variety applications. Different wet-chemistry techniques already exist fix resultant polymeric structure predictable manner. In this work, an all-dry and plasma-based strategy is proposed fabricate thin films microphase-separated polyolefin/polyether blends. This achieved by directing (−CH2−)100 (−CH2–CH2–O−)25 oligomer fluxes produced vacuum thermal decomposition...
Fusion energy has the potential to provide sustainable solutions global needs for next generations. However, despite decades of intense international efforts many scientific and technological breakthroughs need be achieved before fusion become available economically viable. In addition, prior industrial development technology, it is worth addressing possible negative environmental health impacts. For instance, interactions between plasma refractory materials called facing components (PFC)...