A new methodology for the preparation of adressed DNA matrices is described. The process includes an electrochemically directed copolymerization pyrrole and oligonucleotides bearing on their 5′ end a moiety introduced by phosphoramidite chemistry. electro-controlledsynthesis copolymer (polypyrrole) gives, in one step, solid conducting film deposited surface electrode. resulting polymer consists chains covalently linked oligonucleotide. growth limited to electrode surface, so that it possible...

Cancer cells develop resistance to chemotherapy, and the side effects encountered seriously limit effectiveness of treatments. For these reasons, search for alternative therapies that target cancer without affecting healthy tissues is currently one most active areas research on cancer. The present study focuses a recently proposed approach cell destruction based targeted triggering spontaneous death through mechanical vibration anisotropic magnetic micro/nanoparticles attached membranes at...

We describe in this paper a methodology to quantify multispot parallel DNA hybridizations and denaturations on gold surfaces by using, one hand, polypyrrole-based surface functionalization based an electrospotting process and, the other plasmon resonance imaging allowing real-time measurements several spots at time. Two characterization steps were performed order optimize immobilization of oligonucleotide probes thus, increase signal-to-noise ratio monitored hybridization signals: first step...

Journal Article Chemical synthesis of a biologically active natural tRNA with its minor bases Get access Didier Gasparutto, Gasparutto Search for other works by this author on: Oxford Academic PubMed Google Scholar Thierry Livache, Livache 1Laboratoire des Sondes Moléculaires, CIS Bio International, Centre d'Etudes Nucléaires de Grenoble85X, 38041 Grenoble Cedex, France Hervé Bazin, Bazin Anne-Marie Duplaa, Duplaa André Guy, Guy Alexander Khorlin, Khorlin + Visiting professor: Engelhardt...

Microbial culture continues to be the most common protocol for bacterial detection and identification in medicine agronomics. Using this process may take days identify a specific pathogen strains. Surface Plasmon Resonance (SPR) is an emerging alternative technology that can used of bacteria using protein microarrays although typical limits are range 103–106 cfu mL−1, which not compatible with Food Safety regulation requirements. In work, we combine concomitant "on-chip" microbial sensitive...

Monitoring volatile organic compounds (VOCs) is an important issue, but difficult to achieve on a large scale and the field using conventional analytical methods. Electronic noses (eNs), as promising alternatives, are still compromised by their performances due fact that most of them rely very limited number sensors use databases devoid kinetic information. To narrow performance gap between human electronic noses, we developed novel optoelectronic nose, which features sensor microarray...

Human cysteine cathepsin S (catS) participates in distinct physiological and pathophysiological cellular processes is considered as a valuable therapeutic target autoimmune diseases, cancer, atherosclerosis, asthma. We evaluated the capacity of negatively charged glycosaminoglycans (heparin, heparan sulfate, chondroitin 4/6-sulfates, dermatan hyaluronic acid) to modulate activity catS. Chondroitin 4-sulfate (C4-S) impaired collagenolytic (type IV collagen) inhibited peptidase (Z-Phe-Arg-AMC)...

Several optical surface sensing techniques, such as Surface Plasmon Resonance (SPR), work by imaging the base of a prism one its faces. However, fundamental concern has not been fully analyzed and understood so far, spatial resolution remains critical controversial issue. In SPR, propagation length L(x) plasmon waves considered limiting factor. Here, we demonstrate that for unoptimized systems geometrical aberrations caused can be more than length. By combining line-scan mode with optimized...

Aptamers are selected DNA ligands that target biomolecules such as proteins. In recent years, they showing an increasing interest potential therapeutic agents or recognition elements in biosensor applications. both cases, the need for characterizing mating between and aptamer either solution immobilized on a surface, is pressing. this context, we have developed kinetic made of micro-arrayed anti-thrombin aptamers to assess parameters interaction. The binding label-free thrombin was monitored...

Silicon photonics can address a variety of applications, from datacom and biosensing to lidars. Recently, this technology has been explored for gas sensing. Detection identification odors remains critical challenge in diverse areas such as air quality, food spoilage, or personal well-being. In work, we present an olfactory sensor based on array 64 biofunctionalized Mach-Zehnder interferometers integrated silicon nitride platform. The ability analyze at ppm level is demonstrated several...

We demonstrate the use of SPR imaging for detection site-specific binding either B or T lymphocyte populations on an electrochemically-grafted antibody array.

In order to construct tools able screen oligosaccharide−protein interactions, we have developed a polypyrrole-based oligosaccharide chip constructed via copolymerization process of pyrrole and pyrrole-modified oligosaccharide. For our study, GAG (glycosaminoglycans) or fragments, which are involved in many fundamental biological processes, were modified by the moiety on their reducing end then immobilized chip. The parallel binding events upperside surface can be simultaneously monitored...

Prevention of foodborne diseases depends highly on our ability to control rapidly and accurately a possible contamination food. So far, standard procedures for bacterial detection require time-consuming cultures plates before the pathogens can be detected identified. We present here an innovative biochip, based direct differential carbohydrate recognitions five closely related Escherichia coli strains, including enterohemorragic E. O157:H7. Our device relies efficient grafting simple...

Developing rapid, high-throughput assays for detecting and characterizing protein-protein interactions is a great challenge in the postgenomic era. We have developed new method that allows parallel analysis of multiple analytes biological fluids suitable medical studies.This technology studying peptide-antibody based on polypyrrole-peptide chips surface plasmon resonance imaging (SPRi). generated chip bearing large panel peptide probes by successive electro-directed copolymerizations...

Bloodstream bacterial infections are life-threatening conditions necessitating prompt medical care. Rapid pathogen identification is essential for early setting of the best anti-infectious therapy. However, load in blood samples from patients with bacteremia too low and under limit detection most methods direct bacteria. Therefore, a preliminary step enabling multiplication required. To do so, cultures still remain gold standard before diagnosis. Bacterial then usually obtained within 24 to...

A dedicated SPR apparatus optimized for individual bacteria observation and a new strategy early detection of microorganisms in growth.

Headspace analysis of highly humid samples remains a challenge for artificial olfaction. Based on surface plasmon resonance imaging and bio-based sensors, the NeOse Pro olfactive analyzer yields multivariate data enhances statistical discrimination capacity odor patterns. However, presence high background signal, such as water vapor from aqueous samples, may deteriorate its discriminant ability. Recently, miniaturized pre-concentrators packed with hydrophobic adsorbent have been developed to...