A5052749205- Microfluidic and Bio-sensing Technologies
- Microfluidic and Capillary Electrophoresis Applications
- Cancer Cells and Metastasis
- Cancer Genomics and Diagnostics
- 3D Printing in Biomedical Research
- Electrohydrodynamics and Fluid Dynamics
- Biosensors and Analytical Detection
- Digital Holography and Microscopy
- Particle Dynamics in Fluid Flows
- Optical Coherence Tomography Applications
- Orbital Angular Momentum in Optics
- Electrowetting and Microfluidic Technologies
- Lattice Boltzmann Simulation Studies
- Nanopore and Nanochannel Transport Studies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Cancer Research and Treatments
- Prostate Cancer Diagnosis and Treatment
- Mass Spectrometry Techniques and Applications
- Prostate Cancer Treatment and Research
- Granular flow and fluidized beds
- Cell Image Analysis Techniques
- Lung Cancer Research Studies
- 3D Shape Modeling and Analysis
- Optical measurement and interference techniques
- Lung Cancer Treatments and Mutations
University of California, Los Angeles
2011-2016
Stanford University
2016
Menlo School
2015
UCLA Health
2013
California NanoSystems Institute
2012
University of California System
2012
Samueli Institute
2012
Université Grenoble Alpes
2009-2010
Institut polytechnique de Grenoble
2009-2010
CEA Grenoble
2009-2010
A blood-based, low cost alternative to radiation intensive CT and PET imaging is critically needed for cancer prognosis management of its treatment. "Liquid biopsies" circulating tumor cells (CTCs) from a relatively non-invasive blood draw are particularly ideal, as they can be repeated regularly provide up date molecular information about the cancer, which would also open key opportunities personalized therapies. Beyond solely diagnostic applications, CTCs subject interest drug development...
Optical microscopy is one of the most widely used diagnostic methods in scientific, industrial, and biomedical applications. However, while useful for detailed examination a small number (< 10,000) microscopic entities, conventional optical incapable statistically relevant screening large populations (> 100,000,000) with high precision due to its low throughput limited digital memory size. We present an automated flow-through single-particle microscope that overcomes this limitation by...
// James Che 1,2 , Victor Yu 1 Manjima Dhar Corinne Renier 2,3 Melissa Matsumoto Kyra Heirich 3 Edward B. Garon 4 Jonathan Goldman Jianyu Rao 5 George W. Sledge 6 Mark D. Pegram Shruti Sheth Stefanie S. Jeffrey 3,6 Rajan P. Kulkarni 1,7 Elodie Sollier 1,2,3 and Dino Di Carlo 1,8,9 Department of Bioengineering, University California, Los Angeles, USA 2 Vortex Biosciences, Menlo Park, Surgery, Stanford School Medicine, Stanford, Hematology & Oncology, UCLA Medical Center, Pathology...
Inertial effect of a flowing fluid, often complicating factor in hydrodynamics, offers way to high-purity, high-throughput shape-based separation manmade particles or biological cells microfluidic channels.
Laser scanning technology is one of the most integral parts today's scientific research, manufacturing, defense and biomedicine. In many applications, high-speed capability essential for a large area in short time multi-dimensional sensing moving objects dynamical processes with fine temporal resolution. Unfortunately, conventional laser scanners are often too slow, resulting limited precision utility. Here we present new type scanner that offers ∼1,000 times higher scan rates than...
In microfluidic systems at low Reynolds number, the flow field around a particle is assumed to maintain fore-aft symmetry, with fluid diverted by presence of particle, returning its original streamline downstream. This current model considers particles as passive components system. However, we demonstrate that finite when inertia taken into consideration, are not elements in but significantly disturb and modify it. response field, translate downstream while rotating. The combined effect...
Circulating tumor cells (CTCs) are important biomarkers for monitoring dynamics and efficacy of cancer therapy. Several technologies have been demonstrated to isolate CTCs with high efficiency but achieve a low purity from large background blood cells. We previously shown the ability enrich volumes through selective capture in microvortices using Vortex Chip. The device consists narrow channel followed by series expansion regions called reservoirs. Fast flow entry gives rise inertial forces,...
Circulating tumor cells (CTCs) represent a temporal "snapshot" of patient's cancer and changes that occur during disease evolution. There is an extensive literature studying CTCs in breast patients, particularly those with metastatic disease. In parallel, there increasing use patient-derived models preclinical investigations human cancers. Yet studies are still limited demonstrating CTC shedding metastasis formation cancer. We used seven orthotopic xenograft (PDOX) generated from...
Lung cancer is the leading cause of cancer-related mortality worldwide. Epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor (TKI) therapies, based on evaluation EGFR mutations, have shown dramatic clinical benefits. mutation assays are mainly performed tumor biopsies, which carry risks, not always successful and give results relevant to timepoint assay. To detect secondary resistance 1st 2nd generation TKIs lead administration a 3rd drug, effective non-invasive monitoring...
We demonstrate the use of SPR imaging for detection site-specific binding either B or T lymphocyte populations on an electrochemically-grafted antibody array.
Abstract Background Therapies for metastatic castration-resistant prostate cancer (mCRPC) include targeting the androgen receptor (AR) with inhibitors (ARIs) and prostate-specific membrane antigen (PSMA). Having ability to detect AR, AR splice variant 7 (AR-V7), or PSMA in circulating tumor cells (CTCs) exosomal cell-free RNA (cfRNA) could be helpful guide selection of appropriate therapy each individual patient. The Vortex Biosciences VTX-1 system is a label-free CTC isolation that enables...
The isolation, analysis, and enumeration of circulating tumor cells (CTCs) from cancer patient blood samples are a paradigm shift for diagnosis, prognosis, treatment monitoring. Most methods used to isolate enumerate these target rely on the expression cell surface markers, which varies between patients, types, tumors, stages. Here, we propose label-free high-throughput platform isolate, enumerate, size CTCs two coupled microfluidic devices. Cancer were purified through Vortex chip...
Laser scanners are essential for scientific research, manufacturing, defense, and medical practice. Unfortunately, often times the speed of conventional laser (e.g., galvanometric mirrors acousto-optic deflectors) falls short many applications, resulting in motion blur failure to capture fast transient information. Here, we present a novel type scanner that offers roughly three orders magnitude higher scan rates than methods. Our scanner, which refer as hybrid dispersion performs...
Promising microfluidic devices are proposed herein to continuously and passively extract plasma from whole human blood. These designs based on the red cells lateral migration resulting cell-free layer locally expanded by geometric singularities, such as an abrupt enlargement of channel or a cavity adjacent channel. After explanation flow patterns, experimentally biologically validated for extraction. They also successively optimized with extraction yields up 17.8% 1:20 blood injected at 100 muL/min.