A5089116898- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Advanced Biosensing Techniques and Applications
- Microfluidic and Bio-sensing Technologies
- Bacteriophages and microbial interactions
- Nanofabrication and Lithography Techniques
- Monoclonal and Polyclonal Antibodies Research
- 3D Printing in Biomedical Research
- Microfluidic and Capillary Electrophoresis Applications
- Gene expression and cancer classification
- DNA and Nucleic Acid Chemistry
- Cell Image Analysis Techniques
- Listeria monocytogenes in Food Safety
- Gold and Silver Nanoparticles Synthesis and Applications
- Bacterial Identification and Susceptibility Testing
- RNA Interference and Gene Delivery
- Nanopore and Nanochannel Transport Studies
- Photonic and Optical Devices
- Chemical Synthesis and Analysis
- Electrochemical sensors and biosensors
- Force Microscopy Techniques and Applications
- Extracellular vesicles in disease
- RNA and protein synthesis mechanisms
- SARS-CoV-2 detection and testing
- Plasmonic and Surface Plasmon Research
Université Grenoble Alpes
2015-2025
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2014-2024
CEA Grenoble
2014-2024
Centre National de la Recherche Scientifique
2014-2024
Agence de la transition écologique
2019-2020
Institut Nanosciences et Cryogénie
2009-2019
Structure et Propriétés d'Architectures Moléculaires
2009-2017
Systèmes Moléculaires et nanoMatériaux pour l'Énergie et la Santé
2014-2017
Université Joseph Fourier
2000-2014
Laboratoire d'Analyse et d'Architecture des Systèmes
2009
Microbial culture continues to be the most common protocol for bacterial detection and identification in medicine agronomics. Using this process may take days identify a specific pathogen strains. Surface Plasmon Resonance (SPR) is an emerging alternative technology that can used of bacteria using protein microarrays although typical limits are range 103–106 cfu mL−1, which not compatible with Food Safety regulation requirements. In work, we combine concomitant "on-chip" microbial sensitive...
Several optical surface sensing techniques, such as Surface Plasmon Resonance (SPR), work by imaging the base of a prism one its faces. However, fundamental concern has not been fully analyzed and understood so far, spatial resolution remains critical controversial issue. In SPR, propagation length L(x) plasmon waves considered limiting factor. Here, we demonstrate that for unoptimized systems geometrical aberrations caused can be more than length. By combining line-scan mode with optimized...
Aptamers are selected DNA ligands that target biomolecules such as proteins. In recent years, they showing an increasing interest potential therapeutic agents or recognition elements in biosensor applications. both cases, the need for characterizing mating between and aptamer either solution immobilized on a surface, is pressing. this context, we have developed kinetic made of micro-arrayed anti-thrombin aptamers to assess parameters interaction. The binding label-free thrombin was monitored...
Gold-mesoporous silica (Au@mSiO2) core–shell nanoparticles (NPs) hold great promise for application in nanomedicine as they enable the photostimulated release of guest molecules located within their pores. In this study, we investigated mechanism a productive one-pot protocol preparation Au@mSiO2 NPs. The formation gold NPs quickly followed by shell eliminates need to isolate and strongly protect cores. A thorough mechanistic analysis provides insights into relative kinetics formation, which...
Site-specific RNA cleavage has received considerable attention over the years. Directed synthesis to append imidazoles or amines both oligonucleotides target specific represents an exciting avenue of research. However, date catalysis by such synthetic constructs, particularly in terms turnover, been difficult observe. This is first report a truly catalytic M2+-independent DNAzyme synthetically modified with and cationic that would seem mimic RNaseA. work now demonstrates how organic...
We demonstrate the use of SPR imaging for detection site-specific binding either B or T lymphocyte populations on an electrochemically-grafted antibody array.
The detection of small molecules by biosensors remains a challenge for diagnostics in many areas like pharmacology, environment or homeland security. main difficulty comes from both the low molecular weight and concentrations most targets, which generally requires an indirect with amplification sandwich procedure. In this study, we combine strategies as Surface Plasmon Resonance imaging (SPRi) signal is obtained use gold nanoparticles sequence engineering split-aptamers, short...
Bloodstream bacterial infections are life-threatening conditions necessitating prompt medical care. Rapid pathogen identification is essential for early setting of the best anti-infectious therapy. However, load in blood samples from patients with bacteremia too low and under limit detection most methods direct bacteria. Therefore, a preliminary step enabling multiplication required. To do so, cultures still remain gold standard before diagnosis. Bacterial then usually obtained within 24 to...
A dedicated SPR apparatus optimized for individual bacteria observation and a new strategy early detection of microorganisms in growth.
Detecting blood biomarkers such as proteins with high sensitivity and specificity is of the utmost importance for early reliable disease diagnosis. As molecular probes, aptamers are raising increasing interest biosensor applications an alternative to antibodies, which used in classical enzyme-linked immuno-sorbent assays (ELISA). We have developed a sensitive antibody-free quantification assay that combines loop-mediated isothermal amplification (LAMP). For proof-of-concept, we consider two...
A DNAzyme, synthetically modified with both primary amines and imidazoles, is found to act as a M2+-independent AP lyase-endonuclease. In the course of cleavage reaction, this DNAzyme forms covalent Schiff base intermediate an abasic site on complementary oligodeoxyribonucleotide. This intermediate, which inferred from NaCNBH3 trapping well cyanide inhibition, does not evidently accumulate because second step, dehydrophosphorylative elimination, fast compared formation. The 5'-product that...
The development of sensitive methods for in situ detection biomarkers is a real challenge to bring medical diagnosis step forward. proof-of-concept remote multiplexed biomolecular interaction through plasmonic optical fiber bundle demonstrated here. strategy relies on optic biosensor designed from 300 µm diameter composed 6000 individual fibers. When appropriately etched and metallized, each exhibits specific properties. surface plasmon resonance phenomenon occurring at the enables measure...
Deoxyribonolactone in DNA is an oxidized abasic site damage that produced by a variety of physical and chemical agents such as γ-irradiation ene−diyne antibiotics. The extent biological significance the lesion are poorly documented due to high lability damaged DNA. chemistry degradation deoxyribonolactone-containing was investigated using oligonucleotides different length (5-, 11-, 23-, 34-mers) which lactone photochemically generated, already reported, from oligonucleotide precursors...
Rapid detection of enterohaemorrhagic E. coli O157:H7 in large range stress conditions occurring food processing.Detection various processing using surface plasmon resonance imaging (SPRi) technique on an antibody microarray was evaluated. The direct method based the culture/capture/measure (CCM) process consists detecting bacteria during enrichment step, which significantly decreases overall assay duration. In optimized culture conditions, this allows specific low CFU ml(-1) <7 h. Detection...
In the context of bacteriophage (phage) therapy, there is an urgent need for a method permitting multiplexed, parallel phage susceptibility testing (PST) prior to formulation personalized cocktails administration patients suffering from antimicrobial-resistant bacterial infections. Methods based on surface plasmon resonance imaging (SPRi) and phase were demonstrated as candidates very rapid (<2 h) PST in broth phase. Biosensing layers composed arrays phages 44AHJD, P68, gh-1 covalently...
Loop-mediated isothermal amplification (LAMP) is an exponential method of DNA strands that more and used for its high performances. Thanks to sensitivity selectivity, LAMP found numerous applications from the detection pathogens or viruses through their genome incorporation as strategy in protein miRNA biomarker quantification. The composed two stages: first one consists transformation into dumbbell structures formed stems loops thanks four primers; then, second stage, only primers are...