A5004888244- Advanced biosensing and bioanalysis techniques
- Advanced Chemical Sensor Technologies
- Olfactory and Sensory Function Studies
- Advanced Biosensing Techniques and Applications
- Biosensors and Analytical Detection
- Material Dynamics and Properties
- Theoretical and Computational Physics
- Neural Networks and Applications
- Gene expression and cancer classification
- Analytical Chemistry and Sensors
- Biochemical Analysis and Sensing Techniques
- Gas Sensing Nanomaterials and Sensors
- Photonic and Optical Devices
- Nanopore and Nanochannel Transport Studies
- Insect Pheromone Research and Control
- DNA and Nucleic Acid Chemistry
- Phase Equilibria and Thermodynamics
- Plasmonic and Surface Plasmon Research
- Polydiacetylene-based materials and applications
- Advanced Fiber Optic Sensors
- Microfluidic and Capillary Electrophoresis Applications
- Blind Source Separation Techniques
- RNA Interference and Gene Delivery
- Advanced Thermodynamics and Statistical Mechanics
- Polymer Surface Interaction Studies
CEA Grenoble
2016-2025
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2016-2025
Université Grenoble Alpes
2016-2025
Centre National de la Recherche Scientifique
2016-2025
Institut Nanosciences et Cryogénie
2010-2024
Institut polytechnique de Grenoble
2024
Interface (United States)
2019-2023
Agence de la transition écologique
2019-2021
CEA Cadarache
2018-2019
Systèmes Moléculaires et nanoMatériaux pour l'Énergie et la Santé
2014-2018
We have found strong supporting evidence for the helical structures of single-stranded nucleic acids by stretching individual molecules polyadenylic acid [poly(A)] and polycytidylic [poly(C)]. Analyzing force versus extension data using a two-state elastic model in which random-coil domains alternate with rigid allows one to extract thermodynamic structural properties. In addition, it also yields moderate low cooperativity helix-coil transition poly(A) poly(C), respectively.
Monitoring volatile organic compounds (VOCs) is an important issue, but difficult to achieve on a large scale and the field using conventional analytical methods. Electronic noses (eNs), as promising alternatives, are still compromised by their performances due fact that most of them rely very limited number sensors use databases devoid kinetic information. To narrow performance gap between human electronic noses, we developed novel optoelectronic nose, which features sensor microarray...
The design of DNA chip experiments utilizes hybridization isotherms relating the equilibrium at surface to composition solution. Within this family, Langmuir isotherm is simplest and most frequently used. This tutorial review summarizes domain validity discusses modifications necessary allow for competitive in bulk surface, probe polydispersity interactions between sites. constant an impenetrable described, as well relative merits melting temperature c50 parameters. relevance various...
Aptamers are selected DNA ligands that target biomolecules such as proteins. In recent years, they showing an increasing interest potential therapeutic agents or recognition elements in biosensor applications. both cases, the need for characterizing mating between and aptamer either solution immobilized on a surface, is pressing. this context, we have developed kinetic made of micro-arrayed anti-thrombin aptamers to assess parameters interaction. The binding label-free thrombin was monitored...
Abstract Multiplexed remote bio‐detection is demonstrated through an optical waveguide assembly coated with interferometric layers. Image conduits (IC)s, composed of 3012 individual cores, are layers tantalum pentoxide (Ta 2 O 5 ) and silica (SiO to transform each core into a sensitive sensor. The spectral response the IC as function refractive index (RI) changes obtained compared simulated one. experimental sensitivities resolutions cores assessed in detection mode by imaging assembly. For...
Protein detection and identification at the single-molecule level are major challenges in many biotechnological fields. Solid-state nanopores have raised attention as label-free biosensors with high sensitivity. Here, we use solid-state nanopore sensing to discriminate two closely related proteins, α-thrombin γ-thrombin. We show that aptamer functionalization improves protein discrimination thanks a significant difference relative current blockade amplitude. To enhance discrimination,...
We study the equilibrium phase diagram of binary mixtures hard spheres as well parallel cubes. A superior cluster algorithm allows us to establish and access demixed for both systems investigate subtle interplay between short-range depletion long-range demixing.
The detection of small molecules by biosensors remains a challenge for diagnostics in many areas like pharmacology, environment or homeland security. main difficulty comes from both the low molecular weight and concentrations most targets, which generally requires an indirect with amplification sandwich procedure. In this study, we combine strategies as Surface Plasmon Resonance imaging (SPRi) signal is obtained use gold nanoparticles sequence engineering split-aptamers, short...
Detecting blood biomarkers such as proteins with high sensitivity and specificity is of the utmost importance for early reliable disease diagnosis. As molecular probes, aptamers are raising increasing interest biosensor applications an alternative to antibodies, which used in classical enzyme-linked immuno-sorbent assays (ELISA). We have developed a sensitive antibody-free quantification assay that combines loop-mediated isothermal amplification (LAMP). For proof-of-concept, we consider two...
Abstract Phytohemagglutinin (PHA) is a seed storage protein and type of lectin originally discovered in the common bean (Phaseolus vulgaris) for its blood-agglutinating effect. Due to interactions with gut epithelia digestive enzymes potential trigger allergic reactions, PHA can lead various symptoms human body. As result, it has been regarded as significant antinutritional factor beans other legumes. While several published works have summarized structural, biochemical, toxicological...
The cell-SELEX method enables efficient selection of aptamers that bind whole bacterial cells. However, after selection, it is difficult to determine their binding affinities using common screening methods because the large size bacteria. Here we propose a simple surface plasmon resonance imaging (SPRi) for aptamer characterization membrane vesicles, called nanosomes, instead Nanosomes were obtained from fragments mechanical cell disruption in order preserve external epitopes bacterium used...
We introduce simple models, inspired by previous models for froths and covalent glasses, with trivial equilibrium properties but dynamical behaviour characteristic of strong glass forming systems. These are also a generalization backgammon or urn to non-constant number particles, where entropic barriers replaced energy barriers, allowing the existence activated processes. formulate mean-field version which keeps most features finite dimensional ones, solve analytically out-of-equilibrium...