A5005681729- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- Enzyme Catalysis and Immobilization
- Amino Acid Enzymes and Metabolism
- Microbial Metabolic Engineering and Bioproduction
- Electrochemical sensors and biosensors
- Identification and Quantification in Food
- Isotope Analysis in Ecology
- Gas Sensing Nanomaterials and Sensors
- Acoustic Wave Resonator Technologies
- Biochemical Acid Research Studies
- Electrokinetic Soil Remediation Techniques
- Photosynthetic Processes and Mechanisms
- Biochemical and biochemical processes
- Microbial metabolism and enzyme function
- Agricultural Development and Policies
- Vibrio bacteria research studies
- Gold and Silver Nanoparticles Synthesis and Applications
- Geophysical and Geoelectrical Methods
- Air Quality Monitoring and Forecasting
- Enzyme-mediated dye degradation
- Diatoms and Algae Research
- Infection Control and Ventilation
- Ichthyology and Marine Biology
- Soybean genetics and cultivation
China Jiliang University
2020-2025
Université Grenoble Alpes
2017-2021
Centre National de la Recherche Scientifique
2017-2021
CEA Grenoble
2017-2021
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2017-2021
Institut Nanosciences et Cryogénie
2017
The worrying emergence of multiple resistance genes to last-resort antibiotics in food animals and human populations throughout the chain relevant environments has been increasingly reported worldwide. Enterobacteriaceae pathogens are considered most common reservoirs such antibiotic (ARGs). Thus, a rapid, efficient accurate detection method simultaneously screen monitor ARGs become an urgent need. Our study developed recombinase polymerase amplification (RPA) assay combined with lateral...
Along with the revolution of Internet Things (IoT), smart, flexible, easily fabricated, and highly sensitive gas sensors are essential for many portable electronics. In this study, a wearable NO2 sensor based on nanomesh structure woven Ti3C2Tx microflakes Bi2S3 nanobelts operated at room temperature was successfully designed prepared. The effects mass ratio between two materials performance were evaluated. embedded inside accordion-like provide large specific surface areas abundant active...
Abstract Phytohemagglutinin (PHA) is a seed storage protein and type of lectin originally discovered in the common bean (Phaseolus vulgaris) for its blood-agglutinating effect. Due to interactions with gut epithelia digestive enzymes potential trigger allergic reactions, PHA can lead various symptoms human body. As result, it has been regarded as significant antinutritional factor beans other legumes. While several published works have summarized structural, biochemical, toxicological...
Pufferfish is a traditional, delicious dish in Asia. However, eating wild or improperly processed pufferfish causes serious poisoning. This study aimed to exploit the high-resolution melting (HRM) method for authenticating four species of Takifugu (Takifugu xanthopterus, T. fasciatus, flavidus, and rubripes). Candidate DNA barcodes, including cytochrome c oxidase subunit I (COI), b (Cytb), control region (D-loop), were analyzed, with COI selected as optimal barcode. An HRM was developed...
Abstract Background α,β-Unsaturated aldehydes are widely used in the organic synthesis of fine chemicals for application products such as flavoring agents, fragrances and pharmaceuticals. In selective oxidation α,β-unsaturated alcohols to corresponding aldehydes, it remains challenging overcome poor selectivity, overoxidation a low atom efficiency chemical routes. Results An E. coli strain coexpressing NADP + -specific alcohol dehydrogenase YsADH oxygen-dependent NADPH oxidase TkNOX was...
Citronellol is a kind of unsaturated alcohol with rose-like smell and its (S)-enantiomer serves as an important intermediate for organic synthesis (-)-cis-rose oxide. Chemical methods are commonly used the citronellol (S)-enantiomer, which suffers from severe reaction conditions poor selectivity. Here, first one-pot double reduction (E/Z)-citral to (S)-citronellol was achieved in multi-enzymatic cascade system: N-ethylmaleimide reductase Providencia stuartii (NemR-PS) selected catalyze...
The detection of small molecules impacts various fields; however, their size and low concentration are usually the cause limitations in detection. Thus, need for biosensors with appropriate probes signal amplification strategies is required. Aptamers selected specifically against targets such as adenosine. possibility to split aptamers parts led original based on sandwich assays. By combining self-assembling oligonucleotide dimers split-aptamer dangling ends a surface plasmon resonance...
Possessing aldehyde and carboxyl groups, glyoxylic acid its ester derivatives serve as platform chemicals for the synthesis of vanillin, (R)-pantolactone, antibiotics or agrochemicals. Methyl glycolate is one by-products in coal-to-glycol industry, we attempted value-added use through enzymatic oxidation methyl to glyoxylate. The cascade catalysis oxidase from Spinacia oleracea (SoGOX), catalase Helicobacter pylori (HpCAT) hemoglobin Vitreoscilla stercoraria (VsHGB) was firstly constructed,...
The importance of yeast old yellow enzymes is increasingly recognized for direct asymmetric reduction (E/Z)-citral to (R)-citronellal. As one the most performing enzymes, enzyme OYE3 from Saccharomyces cerevisiae S288C exhibited complementary enantioselectivity (E)-citral and (Z)-citral, resulting in lower e.e. value (R)-citronellal (E/Z)-citral. To develop a novel approach synthesis enantio-pure (E/Z)-citral, was firstly modified by semi-rational design improve its (R)-enantioselectivity....
Small molecules are ubiquitous in nature and their detection is relevant various domains. However, due to size, sensitive selective probes difficult select the methods generally indirect. In this study, we introduced use of melting curve analysis aptachains based on split-aptamers for adenosine. Aptamers, short oligonucleotides, known be particularly efficient compared antibodies thanks advantageous probe/target size ratio. Aptachains formed from dimers with dangling ends followed by...
Summary Smart agriculture is based on latest technologies in communication, big data analysis, high-performance sensors, and automatic machinery to improve the effectiveness of industry. China looking forward achieve modernization its industry through smart agriculture. But unique climate, landscape, diversity crops, large population make this task more challenging compared other counties. Herein, we present current achievements regarding policies made guide support development agriculture,...
Prostate-specific antigen (PSA) is a well-established tumour marker for prostatic carcinoma. In this study, we present novel, real-time, and ultrasensitive Love-mode surface acoustic wave (L-SAW) immunosensor PSA detection enhanced by MoS
The cascade catalysis of old yellow enzyme, alcohol dehydrogenase and glucose has become a promising approach for one pot, two-step reduction (E/Z)-citral to (S)-citronellol, serving as chiral with rose fragrance. During the multi-enzymatic catalysis, enzyme is responsible conjugated C=C introduction center, requiring high activity (S)-enantioselectiviy. Herein, improve from Providencia stuartii (NemR-PS) strict (S)-enantioselectivity, semi-rational design on its substrate binding pocket was...
Allopolyploids play an essential role in plant evolution and confer apparent advantages on crop growth breeding compared to low ploidy levels. A doubled haploid (DH) population derived from the cross between two artificially synthesized allohexaploid Brassica was created self-crossed continuously. Morphological yield-related traits showed considerable variation among different generations, families even within same families. However, flowering time, pollen viability seed yield increased...
Abstract Background: α,β-unsaturated aldehydes are widely used in as organic synthesis of fine chemicals such flavor, fragrances and pharmaceuticals. The selective oxidation alcohols to the corresponding remains challenging overcome poor selectivity, over-oxidation low atom efficiency chemical routes. Results: An E. coli strain co-expressing NADP + -specific alcohol dehydrogenase YsADH oxygen-dependent NADPH oxidase TkNOX was constructed, which enabled regeneration catalyzed 100 mM...