Fetal growth restriction (FGR) is a significant pregnancy condition characterized by the fetus failing to attain its full genetic potential. FGR primarily ascribed defective placentation, owing impaired trophoblast cellular function. The objective of this research elucidate pathogenic functions suppressor Mek1 (SMEK1) in FGR. Western blot and Immunofluorescence were used detect expression localization SMEK1 placenta. We overexpressed knocked down using plasmid or siRNA special targeted it....

Introduction Gestational diabetes mellitus (GDM), a common complication of pregnancy, is risky for both mother and fetus. Previous studies about TP53-induced glycolysis apoptosis regulator (TIGAR) focused on the occurrence development cancer, cardiovascular disease, neurological however, it still unclear whether TIGAR plays regulatory role in gestational (GDM). Methods Utilizing HG exposure, we explored oxidative stress limitation, excessive inflammatory toxicity defense, pyroptosis...

Rationale: Acute lung injury (ALI)/acute respiratory distress syndrome (ARDS) is a critical with mortality rate of up to 40%, and it characterized by prominent inflammatory cascade. The inflammasome pyroptosis play crucial regulatory roles in regulating various inflammatory-related diseases serving as pivotal signaling platforms for responses mediating the release substantial quantities factors. Our previous studies confirmed that GC-1, clinical-stage thyroid hormone analog, effectively...

ObjectiveCervical cancer remains a global health challenge, particularly among elderly patients aged 65 and above, who often face poorer outcomes. This study aims to evaluate the clinical significance of chemotherapy in (≥65 years) undergoing definitive radiotherapy for cervical cancer, using data from SEER database.MethodsA retrospective cohort was conducted 18 Registries, focusing on older diagnosed between 2004 2017. Patients receiving surgery or those without were excluded....

Porcine reproductive and respiratory syndrome (PRRS) is caused by the PRRS virus (PRRSV). It leads to disorders failure in sows piglets. As an endoribonuclease, PRRSV non-structural protein 11 (NSP11) crucial replication assists immune system evasion. NSP11, which relatively more conserved than NSP2, could potentially cause a new round of epidemics, given major mutation occurs. Here, we aimed analyze PRRSV-2 NSP11 genetic diversity China between 1996‒2022. The nucleotide sequence was...

Introduction Preeclampsia (PE) is a hypertensive disorder of pregnancy characterized by pronounced placental oxidative stress and inflammatory damage. However, the contribution mitophagy to inflammation-induced injury in PE remains unclear. Methods Human placenta samples were collected from 15 normal pregnant women preeclampsia women. Protein expression was analyzed western blotting, while immunofluorescence staining employed localize mediators. Mitochondrial reactive oxygen species...

Hyperglycemia-induced neuronal apoptosis is one of the important reasons for diabetic neuropathy. Long time exposure to high glucose accelerates many aberrant metabolic pathways and eventually leads injury. However, underlying mechanisms alterations remain unknown. TP53 inducible glycolysis regulator (TIGAR) an endogenous inhibitor increases flux pentose phosphate pathway (PPP) by regulating 6-phosphate dehydrogenase (G6PD). TIGAR highly expressed in neuron. But role hyperglycemia-induced...

The vegetable soybean (Glycine max L. Merr.) plant is commonly consumed in Southeast Asian countries because of its nutritional value and desirable taste. A "pandan-like" aroma an important value-added quality trait that rarely found commercial varieties. In this study, three novel aromatic cultivars with a fragrant volatile compound were isolated. We confirmed the these due to potent 2-acetyl-1-pyrroline (2AP) was previously identified soybean. sequence comparison GmBADH1/2 (encoding...

Abstract A pyramid strategy combining the Cry1A and Cry2A toxins in Bt crops has been widely used throughout world to delay pest adaption transgenic broaden insecticidal spectrum. Midgut membrane-bound cadherin (CAD), aminopeptidase-N (APN) alkaline phosphatase (ALP) are important for toxicity some lepidopteran larvae, but proteins that bind midgut of target insects their role mechanism action still unclear. In this study, we found heterologously expressed CAD, APN4 ALP2 peptides from...

PE is a pregnancy-specific syndrome and one of the main causes maternal, fetal, neonatal mortality. PRDX1 an antioxidant that regulates cell proliferation, differentiation, apoptosis. The aim this study to investigate effect on regulation trophoblast function by affecting autophagy oxidative stress in preeclampsia.Western blotting, RT-qPCR, immunofluorescence were used examine expression placentas. PRDX1-siRNA was transfected knockdown HTR-8/SVneo cells. biological cells detected wound...

Physalis spp. develop the "Chinese lantern" trait, also known as inflated calyx syndrome, that is a morphological novelty. Here, we identified double-layered-lantern1 (doll1) mutant, recessive and monofactorial mutation, in floridana; its corolla androecium were transformed into gynoecium, respectively. Two GLOBOSA-like MADS-box paralogous genes PFGLO1 PFGLO2 found floridana, while mutated phenotype was cosegregated with large deletion harboring complemented by genomic locus transgenic...

The members of the Old Yellow Enzyme (OYE) family are capable catalyzing asymmetric reduction (E/Z)-citral to (R)-citronellal-a key intermediate in synthesis L-menthol. applications OYE-mediated biotransformation usually hampered by its insufficient enantioselectivity and low activity. Here, (R)-enantioselectivity from Saccharomyces cerevisiae CICC1060 (OYE2y) was enhanced through protein engineering. single mutations OYE2y revealed that sites R330 P76 could act as switch OYE2y....

Epidemiological studies have demonstrated that fine particulate matter (PM2.5) is associated with adverse obstetric and postnatal metabolic health outcomes, but the mechanism remains unclear. This study aimed to investigate toxicological pathways by which PM2.5 damaged placental trophoblasts in vivo vitro. We confirmed induced gestational outcomes such as increased fetal mortality rates, decreased numbers weight, structure, apoptosis of trophoblasts. Additionally, dysfunction trophoblast...

Homocysteine methyltransferase (HMT) converts homocysteine to methionine using S-methylmethionine (SMM) or S-adenosylmethionine (SAM) as methyl donors in organisms, playing an important role supplying for the growth and development of plants. To better understand functions HMT genes plants, we conducted a wide evolution expression analysis these genes. Reconstruction phylogenetic relationship showed that gene family was divided into Class 1 2. In 1, HMTs were only found seed while 2...

Hypoxic-ischemic encephalopathy (HIE) is one main cause of neonatal death and disability, causing substantial injury to white gray matter, which can lead severe neurobehavioral dysfunction, including intellectual disability dyskinesia. Inflammation, nerve cell death, matter are important factors in the pathological process HIE. 6-Gingerol a ginger extract, reduces inflammatory response death. However, role hypoxic-ischemic brain (HIBI) remains unknown. In this study, we constructed mouse...

The DA1 gene family is plant-specific and Arabidopsis regulates seed organ size, but the functions in soybeans are unknown. cultivated soybean (Glycine max) believed to be domesticated from annual wild soja). To evaluate whether DA1-like genes were involved evolution of soybeans, we compared variation at both sequence expression levels G. max (GmaDA1) soja (GsoDA1).Sequence identities extremely high between orthologous pairs while paralogous copies a species showed relatively divergence....

Unsaturated fatty acids are the main components of vegetable oils. Fatty acid desaturase 2 (FAD2) catalyzes oleic (OA) into linoleic (LA) transformations, which essential to profile FAs in seeds. To further understand roles FAD2s synthesis oil, evolution and biocatalysis were comprehensively analyzed. The history FAD2 gene family showed that most genes formed monophyletic clades except eudicots. some eudicots diverged constitutive seed-specific expression clades. Notably, or -abundant...

Reference genes are the key to study gene expression patterns using quantitative real-time PCR (qRT-PCR). No studies on reference of Athetis dissimilis , an important agricultural pest, have been reported. In order determine for qRT-PCR normalization in A. under different conditions, 10 candidate [18S ribosomal protein ( 18S ), 28S arginine kinase AK elongation factor 1 alpha EF1-α glyceraldehyde-3-phosphate dehydrogenase GAPDH L32 RPL32 L40 RPL40 alpha-tubulin α-TUB beta-actin β-ACT and...

Propylea japonica (Coleoptera: Coccinellidae) is a natural enemy insect with wide range of predation in mainland China and commonly used pest management. However, its genetic pattern (i.e., variation, structure, historical population dynamics) still unclear, impeding the development biological control pests. Population research has potential to optimize strategies at different stages processes. This study 23 nuclear microsatellite sites mitochondrial COI genes investigate genetics based on...

Soybean is one of the important economic crops, which supplies a great deal vegetable oil and proteins for human. The content nutrients in different soybean seeds different, related to expression multiple genes, but mechanisms are complicated still largely uncertain. In this study, reveal possible causes difference soybeans A7 (containing low high protein) A35 protein), RNA-seq technology was performed compare identify potential differential expressed genes (DEGs) at seed developmental...