Inorganic phosphate (Pi) is stored in the vacuole, allowing plants to adapt variable Pi availability soil. The transporters that mediate sequestration into vacuole remain unknown, however. Here we report functional characterization of Vacuolar Phosphate Transporter 1 (VPT1), an SPX domain protein transports Arabidopsis. vpt1 mutant were stunted and consistently retained less than wild type plants, especially when grown medium containing high levels Pi. In seedlings, VPT1 was expressed...

Abstract The bZIP transcription factor (TF) act as an important regulator for the abscisic acid (ABA) mediated abiotic stresses signaling pathways in plants. Here, we reported cloning and characterization of GhABF2 , encoding typical cotton TF. Overexpression significantly improved drought salt stress tolerance both Arabidopsis cotton. However, silencing made transgenic sensitive to PEG osmotic stress. Expression was induced by ABA treatments but repressed high salinity. Transcriptome...

Penicillium expansum, as a main postharvest pathogen of fruits, can secrete patulin (PAT), causing fruit decay and health problems. In this study, the antifungal test, SEM (scanning electron microscope) observation, transcriptional profile, PAT biosynthesis, physiological characters P. expansum exposed to cinnamaldehyde citral combination (Cin/Cit) were evaluated. Cin/Cit could inhibit mycelial growth spore germination in dose-dependent manner. Besides, caused spores mycelia wrinkled...

Although arsenite [As(III)] is non-essential and toxic for plants, it effectively absorbed through various transporters into the roots. Here we identified a calcium-dependent protein kinase (CPK31) response As(III) tolerance in Arabidopsis. We CPK31 as an interacting of nodulin 26-like intrinsic (NIP1;1), aquaporin involved uptake. Similarly to nip1;1 mutants, loss-of-function mutants improved against but not As(V), accumulated less roots than that wild-type plants. The...

Cinnamaldehyde (Cin) and citral (Cit) have been studied as antimicrobial agents natural preservatives, but their action modes are controversial, the knowledge of antifungal mechanism against P. expansum is still incomplete. The present study was conducted to evaluate combination Cin Cit (Cin/Cit) by observing cellular ultrastructure, fourier transform infrared spectroscopy (FT-IR) fingerprints, plasma membrane, oxidative stress volatile profile. Cin/Cit caused membrane invaginations,...

FERONIA (FER), a plasma membrane receptor-like kinase, is central regulator of cell growth that integrates environmental and endogenous signals. A peptide ligand rapid alkalinization factor 1 (RALF1) binds to FER triggers series downstream events, including inhibition Arabidopsis H+-ATPase 2 activity at the surface regulation gene expression in nucleus. We report here that, upon RALF1 binding, first promotes ErbB3-binding protein (EBP1) mRNA translation then interacts with phosphorylates...

Diabetes, associated with hyperlipidemia and oxidative stress, would lead to an increased production of reactive oxygen species. Rehmannia glutinosa (Di Huang) is widely used nourish yin, invigorate the kidney (shen), treat xiao ke (a diabetes-like syndrome in Chinese medicine). This study aims investigate antidiabetic antioxidant effects catalpol from R. on rat diabetes induced by streptozotocin (STZ) high-fat, high-sugar feed.Rats (eight rats each group at least) were initial high-fat feed...

Abscisic acid (ABA) has been known participate in a wider range of adaptive responses to diverse environmental abiotic stresses such as drought, osmosis, and low temperatures. ABA signaling is initiated by its receptors PYR/PYL/RCARs, type soluble proteins with conserved START domain which can bind trigger the downstream pathway. Previously, we discovered that poplar (Populus trichocarpa) genome encodes 14 PYR/PYL/RCAR orthologues (PtPYRLs), two them, PtPYRL1 PtPYRL5 have functionally...

Abstract Sex determination systems in plants can involve either female or male heterogamety (ZW XY, respectively). Here we used Illumina short reads, Oxford Nanopore Technologies (ONT) long reads and Hi‐C to assemble the first chromosome‐scale genome of a willow tree ( Salix dunnii ), predict genes using transcriptome sequences available databases. The final sequence 328 Mb total was assembled 29 scaffolds, includes 31,501 predicted genes. Analyses short‐read data that included suggested...

Abstract The role of exogenous methyl jasmonate (MeJA) in alleviating drought stress was investigated on Huangguogan. Except for intercellular CO2 concentration, MeJA had little effect net photosynthetic rate, stomatal conductance, and transpiration rate under stress. Compared with stress, significantly alleviated the decrease chlorophyll content. However, a/b ratio increased. increased proline soluble sugar contents, decreased O2 −· H2O2 levels, SOD POD activities. In addition, MDA content...

Alfalfa (Medicago sativa L.) is an important forage crop, and its productivity severely affected by salt stress. Although proline a compatible osmolyte that plays role in regulating plant abiotic stress resistance, the basic mechanism of requires further clarification regarding effect mitigating harmful effects salinity. Here, we investigate protective regulatory mechanisms on tolerance alfalfa. The results show exogenous obviously promotes seed germination seedling growth salt-stressed Salt...

Pepper is one of the most vitamin C enriched vegetables worldwide. Although applying nitrogen (N) fertilizer an important practice for high fruit yield in pepper production, it still unclear how N application regulates anabolism at different maturity stage. To further understanding, we combined physiological and metabolomic analysis to investigate content (including ascorbic acid (AsA) dehydroascorbic (DHA)), related enzyme activity non-targeted metabolites field-grown chili produced under...

Abstract Polyploids recurrently emerge in angiosperms, but most polyploids are likely to go extinct before establishment due minority cytotype exclusion, which may be specifically a constraint for dioecious plants. Here we test the hypothesis that stable sex‐determination system and spatial/ecological isolation facilitate of polyploids. We determined ploidy levels 351 individuals from 28 populations species Salix polyclona , resequenced 190 S . related taxa genomic diversity analyses. The...

This study investigates the role of arbuscular mycorrhizal (AM) fungi, particularly Rhizophagus irregularis, in enhancing lead (Pb) stress tolerance poplar trees (Populus simonii × P. nigra ‘1307’). Under Pb conditions (0, 250, and 750 mg/kg), AM fungi significantly improved growth, evidenced by increases plant height (25.4%–31.2%), shoot biomass (21.6%–37.8%), root (28.9%–41.7%). Photosynthetic parameters, including net photosynthetic rate (Pn), stomatal conductance (Gs), transpiration...

Chromium salt fillers commonly used in current anti-corrosion coatings are highly toxic. However, due to the unique high–low valence transformation and passivation mechanisms of chromium-based functional their wide applicability, chromium-free find it challenging achieve same performance industry acceptance. This study introduces an innovative approach that uses zinc reduce molybdate (MoO42−) acidic solution, thereby forming a multivalent MoO42− system (PMZ system), applies insulating...

Abstract Several puncture leaks and perforation failures occurred in the water injection pipeline outside a joint station western oilfield, seriously affecting pipeline’s integrity. By analyzing clogging pipeline, it was found that corrosion caused by salt scale inside main reason for failure of station. Given serious scaling station, multi-functional agent inhibitor, suppressant developed. It determined had best effect when ratio single-agent additive 2:3:5. The sum three is 87% formula. At...

Parturition and lactation stress greatly affect physiological metabolic status of postpartum cows. Monitoring feeding behavior can help assess the health This study aimed to explore changes in behavior, milk yield (MY), serum indexes, metabolites dairy cows during three weeks postpartum. Furthermore, relationships between were investigated. One hundred seventy-eight healthy multiparous Holstein enrolled continuously record variables, including time (FT), frequency (FF), average (AFT), using...

Inositol polyphosphate 5-phosphatase (5PTase) is a key enzyme in the phosphatidylinositol metabolic pathway, which plays critical roles number of cellular processes plants. Our previous work implicated role 5PTase13, encodes WD40-containing type II 5PTase, hormone-mediated cotyledon vein development. Here, we show that 5PTase13 also involved blue light responses Arabidopsis thaliana. Compared with darkness, expression was suppressed by irradiation, and disruption gene resulted shortened...

Auxin controls a myriad of plant developmental processes and response to environmental conditions. Precise trafficking auxin transporters is essential for homeostasis in plants. Here, we report characterization Arabidopsis CTL1, which seedling growth apical hook development by regulating intracellular PIN-type transporters. The CTL1 gene encodes choline transporter-like protein with an expression pattern highly correlated distribution enriched shoot root meristems, lateral primordia, the...