A5101740626- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Aquaculture disease management and microbiota
- Plant nutrient uptake and metabolism
- Invertebrate Immune Response Mechanisms
- Plant Micronutrient Interactions and Effects
- Plant Reproductive Biology
- Photosynthetic Processes and Mechanisms
- Molecular Biology Techniques and Applications
- Protist diversity and phylogeny
- Toxin Mechanisms and Immunotoxins
- Biological and pharmacological studies of plants
- Protein Hydrolysis and Bioactive Peptides
- CRISPR and Genetic Engineering
- Bacteriophages and microbial interactions
- interferon and immune responses
- Plant Virus Research Studies
- Polysaccharides and Plant Cell Walls
- Medical Research and Treatments
- MicroRNA in disease regulation
- Genomics and Phylogenetic Studies
- Aluminum toxicity and tolerance in plants and animals
- Biopolymer Synthesis and Applications
- Light effects on plants
- Ecology and Conservation Studies
University of California, Berkeley
1998-2021
Plants respond to environmental stress by activating “stress genes.” The plant hormone abscisic acid (ABA) plays an important role in stress-responsive gene expression. Although Ca2+ serves as a common second messenger signaling and ABA, little is known about the molecular basis of action these pathways. Here, we show that CIPK3, Ser/Thr protein kinase associates with calcineurin B–like calcium sensor, regulates ABA response during seed germination ABA- stress-induced expression Arabidopsis....
CLE peptides, named for the CLV3/ESR-related peptide family, participate in intercellular-signaling pathways. Here we investigated members of CLE-like (CLEL) gene family that encode precursors recently designated as root growth factors [Matsuzaki Y et al. (2010) Science 329:1065–1067]. CLEL share a similar domain structure with (i.e., they contain putative N-terminal signal and C-terminal conserved 13-amino-acid motif variable middle portion). Our evidence shows that, unlike factor, peptides...
Auxin controls a myriad of plant developmental processes and response to environmental conditions. Precise trafficking auxin transporters is essential for homeostasis in plants. Here, we report characterization Arabidopsis CTL1, which seedling growth apical hook development by regulating intracellular PIN-type transporters. The CTL1 gene encodes choline transporter-like protein with an expression pattern highly correlated distribution enriched shoot root meristems, lateral primordia, the...
Summary Immuonosuppressive drugs FK506 and rapamycin block a number of signal transduction pathways in eukaryotic systems. The 12 kDa binding protein (FKBP12) mediates the action both against their functional targets. In this report, we cloned, sequenced characterized gene encoding FKBP12 Vicia faba ( Vf FKBP12). While is highly homologous to animal yeast FKBP12, it does not mediate rapamycin. There are unique features plant sequences that cause variation function. One lies domain critical...
Multiple transporters and channels mediate cation transport across the plasma membrane tonoplast to regulate ionic homeostasis in plant cells. However, much less is known about molecular function of that facilitate other organelles such as Golgi. We report here Arabidopsis KEA4, KEA5, KEA6, members cation/proton antiporters-2 (CPA2) superfamily were colocalized with Golgi marker, SYP32-mCherry. Although single kea4,5,6 mutants showed similar phenotype wild type under various conditions,...
Magnesium (Mg2+ ) is an essential metal for plant growth; however, its over-accumulation in cells can be cytotoxic. The tolerance protein family (MTP) belongs to ubiquitous of cation diffusion facilitator (CDF) proteins that export divalent cations homeostasis and all organisms. We describe here the identification MTP10 critical xylem Mg Arabidopsis under high Mg2+ conditions. contains 12 MTP genes, only knockout decreased high-Mg stress. functional complementation assays a -uptake-deficient...
Abstract Nitrate (NO 3 − ) is a source of plant nutrients and osmolytes, but its delivery machineries under osmotic low‐nutrient stress remain largely unknown. Here, we report that AtICln, an Arabidopsis homolog the nucleotide‐sensitive chloride‐conductance regulatory protein family (ICln), involved in response to low‐NO stress. The gene AtICln , encoding plasma membrane–anchored proteins, was upregulated by various stresses, disruption impaired tolerance Compared with wild type, aticln...
研究拟探究经热灭活的白斑综合征病毒 (White spot syndrome virus, WSSV) 能否诱导中国明对虾 (Fenneropenaeus chinensis) 产生免疫致敏反应。将具有典型白斑综合征症状的对虾肌肉剁碎后经60℃灭活1 h，采用单尾定量口饲的方法，连续6 d投喂不同水温条件 (15℃、23℃、28℃、32℃) 下的实验组对虾 (E15℃、E23℃、E28℃、E32℃)；同时设置常温 (23℃) 条件下阳性对照组C23℃(投喂未经灭活的WSSV毒饵)、对照组CF23℃(只投喂商业配合饲料)。在实验第13天对存活个体进行第二次人工WSSV感染，结果显示，WSSV经60℃处理1 h可彻底失活，表现为连续投喂6 d至第二次人工感染期间无对虾死亡，而阳性对照组C23℃死亡率为100%。截止实验第19天，E15℃、E23℃、E28℃、E32℃各组存活率分别为80.41%、33.29%、8.47%、16.43%，CF23℃组的存活率为8.89%，E15℃组与其他各实验组差异极显著 (P...
本研究以中国明对虾(Fenneropenaeus chinensis)为对象，探讨不同浓度的聚β-羟基丁酸酯(Poly-β-hydroxybutyrate，PHB)对其非特异性免疫相关酶的影响。实验采用单因子浓度梯度法，对健康的中国明对虾投喂添加不同浓度PHB (0、0.5%、1.0%、2.5%、5.0%、10.0%)的饲料，分别对应对照组C和实验组E0.5、E1.0、E2.5、E5.0、E10.0组，饲喂6周，统计每组中国明对虾的死亡率和相对免疫保护率，检测总抗氧化能力(T-AOC)、酸性磷酸酶(ACP)、过氧化物酶(POD)和过氧化氢酶(CAT)、超氧化物歧化酶(SOD) 5种酶的活力及丙二醛(MDA)含量与时间、PHB浓度的变化关系。结果显示，实验组的相对免疫保护率随PHB浓度的增加呈现先上升后下降的趋势。E1.0组为最高值，并且与其他各组相比差异显著(P <...
采用单因子浓度梯度法，在中国对虾(Fenneropenaeus chinensis)的生长和产卵阶段，对健康的中国对虾亲虾投喂添加不同含量聚β-羟基丁酸酯(PHB)(0、0.5%、1.0%、2.0%、3.0%、5.0%)的饵料，以期探究不同含量PHB对中国对虾亲虾的繁殖性能和幼体发育的影响。实验周期为192 d，统计亲虾的死亡率、相对免疫保护率、增重率、特定生长率、性腺发育周期、产卵量、无节幼体的数量、孵化率及变态发育周期。结果显示，PHB对中国对虾亲虾的繁殖性能和幼体发育均有一定影响。随着PHB浓度的升高，死亡率呈先下降后上升趋势，而相对免疫保护率则呈现相反的变化趋势，其中，2.0%浓度组的死亡率最低，2.0%与1.0%浓度组差异不显著(P > 0.05)，但与其他浓度组相比均差异显著(P 0.05)。实验组产卵量和无节幼体数量的平均值高于对照组，其中，2.0%浓度组与对照组相比差异显著(P 0.05)。对照组与各实验组在幼体的变态发育时间无显著性差异(P 0.05)。研究表明，PHB添加剂对中国对虾亲虾的繁殖性能和幼体发育具有促进作用，2.0%为最适浓度。
Bantam能够调控细胞增殖、细胞凋亡等过程，影响生物的免疫过程。本研究利用实时荧光定量PCR(qRT-PCR)技术对感染WSSV的中国明对虾(Fenneropenaeus chinensis)肝胰腺和鳃组织内bantam表达水平进行检测，发现感染WSSV后6、12、24和48 h，中国明对虾肝胰腺中的bantam表达水平分别是对照组的(0.16±0.03)(P < 0.05)、(0.63±0.26)、(0.32±0.06)(P 0.05)和(0.41±0.13)倍；中国明对虾鳃中的bantam表达水平分别是对照组的(0.30±0.17)(P 0.05)、(1.88±0.26)(P...
醛缩酶(FBA)是糖酵解和糖异生中的关键酶，参与多种生物过程。本研究采用RACE技术，克隆获得中国明对虾(Fenneropenaeus chinensis)FBA基因(FcFBA)的全长cDNA序列，并对其进行生物信息学分析。结果显示，中国明对虾FcFBA基因的cDNA全长为2496 bp，其中，ORF长1098 bp，5' UTR长79 bp，3' UTR长1319 bp。完整的阅读框编码365个氨基酸，分子量为39.8 kDa，预测的理论等电点为6.6。同源性及系统进化分析表明，FcFBA与节肢动物的FBA聚为一类，与卤虫(Artemia franciscana)、家蚕(Bombyx mori)、沙漠蝗(Schistocerca gregaria)的相似度分别是86%、79%和78%。荧光定量PCR结果显示，FcFBA在肌肉中的相对表达量最高，肝胰腺中最低。WSSV感染后，该基因在鳃、肝胰腺和肌肉中呈现出不同的时空表达特点。dsRNA干扰24...