Abstract Cellular metabolism plays a crucial role in controlling the proliferation, differentiation, and quiescence of neural stem cells (NSCs). The metabolic transition from aerobic glycolysis to oxidative phosphorylation has been regarded as hallmark neuronal differentiation. Understanding what triggers reprogramming how glucose directs NSC differentiation may provide new insight into regenerative potential brain. TP53 inducible apoptosis regulator (TIGAR) is an endogenous inhibitor highly...

Relationships between iron-dependent ferroptosis and nerve system diseases have been recently revealed. However, the role of in neuropathic pain (NeP) remains to be elucidated. Thus, we aimed investigate whether spinal cord contributes NeP induced by a chronic constriction injury (CCI) sciatic nerve.Forty Sprague-Dawley rats received CCI or sham surgery, were randomly assigned following four groups: group; + LIP Veh group. Liproxstatin-1 corn oil separately injected intraperitoneally for...

Peripheral nerve damage causes neuroinflammation, which plays a critical role in establishing and maintaining neuropathic pain (NeP). The mechanisms contributing to neuroinflammation remain poorly elucidated, pharmacological strategies for NeP are limited. Thus, this study, we planned explore the possible link between astrocyte senescence disorders following chronic sciatic injury.An animal model was established by inducing constrictive injury (CCI) adult rats. A senolytic drug combination...

Electrical stimulation holds promise for enhancing neuronal differentiation of neural stem cells to treat traumatic brain injury. However, once the leave stimulating material and migrate post transplantation, electrical on them is diminished. Here, we wrap with wireless nanopatches, conductive graphene nanosheets. Under electromagnetic induction, can thus be applied in-situ individual nanopatch-wrapped demand, their through a MAPK/ERK signaling pathway. Consequently, 41% differentiate into...

Titin, essential for mechano-homeostasis in cardiac and skeletal sarcomere, contains numerous mechanosensitive immunoglobulin-like (Ig) domains its I-band region. However, how proline isomerization cysteine-mediated disulfide bond collectively regulate Ig domain dynamics within the physiological force range remains unclear. Here, we use single-molecule spectroscopy to quantify proximal Ig1 domain, revealing that leads two native states–trans cis states–with distinct mechanical thermal...

Prenatal stress (PNS) is a significant risk factor impacting the lifelong health of offspring, and it has been widely recognized as being closely linked to increased prevalence neurodevelopmental disorders psychiatric illnesses. However, effective pharmacological interventions mitigate its detrimental effects remain limited. Melatonin (Mel), an endogenous hormone, demonstrated considerable potential in treating neurological diseases due anti-inflammatory, antioxidant, neuroprotective...

Abstract Colorectal cancer (CRC) stem cells are resistant to therapy and therefore responsible for tumour progression after conventional fails. However, the molecular mechanisms underlying maintenance of stemness poorly understood. In this study, we identified PCGF1 as a crucial epigenetic regulator that sustains cell-like phenotype CRC. expression was increased in CRC significantly correlated with poor prognosis patients. knockdown inhibited cell proliferation enrichment. Importantly,...

Colorectal cancer (CRC) has a significant burden on healthcare systems worldwide, and is associated with high morbidity mortality rates in patients. In 2020, the estimated new cases of colon United States are 78,300 men 69,650 women. Thus, developing effective novel alternative agents adjuvants reduced side effects important to reduce lethality disease improve quality life Melatonin, pineal hormone that possesses numerous physiological functions, including anti‑inflammatory antitumor...

Temporomandibular joint osteoarthritis (TMJ OA) is characterized by the degeneration of cartilage and subchondral bone. In this study, we observed a significant increase in cell-free DNA (cfDNA) levels during progression TMJ OA. Bioinformatics analysis identified TLR9 as pivotal molecule OA pathogenesis. The polyamidoamine (PAMAM) dendrimer well-structured, highly branched, reactive nature, exhibits robust binding clearance capabilities for cfDNA. However, abundant amino groups on surface...

Soybean (Glycine max) is an important economic crop that provides abundant oil and high quality protein for human beings. As the process of reproductive growth directly determines seed yield quality, we initiated studies to identify genes regulate soybean floral organ development. One R2R3-MYB transcription factor gene, designated as GmMYB181, was found be enriched in flowers based on microarray analysis further functionally investigated transgenic Arabidopsis. GmMYB181 contains two MYB...

Glioblastoma stem-like cells (GSCs) are hypothesized to contribute self-renewal and therapeutic resistance in glioblastoma multiforme (GBM) tumors. Constituting only a small percentage of cancer cells, GSCs possess "stem-like", tumor-initiating properties display irradiation chemotherapy. Thus, novel approaches that can be used suppress urgently needed. A new carbon material—graphene oxide (GO), has been reported show potential for use tumor therapy. However, the exact effect GO on inherent...

Pain and psychological disorders are the 2 most commonly occurring symptom clusters in patients with postherpetic neuralgia (PHN). This study aimed to investigate risk factors for anxiety depressive PHN.Retrospectively, we examined potential of depression among PHN from clinic medical records Third Affiliated Hospital Sun Yat-Sen University 2017 2019. The Chinese version Anxiety Depression Scale was used assess depression. Patients were retrospectively allocated groups - without...

Growing evidence indicates that postnatal immune activation (PIA) can adversely increase the lifetime risk for several neuropsychiatric disorders, including anxiety and depression, which involve of glial cells early neural developmental events. Several glia-targeted agents are required to protect neonates. Folic acid (FA), a clinical medication used during pregnancy, has been reported have neuroprotective properties. However, effects mechanisms FA in PIA-induced neonatal encephalitis mood...

Previous studies have confirmed the relationship between iron-dependent ferroptosis and a peripheral nerve injury-induced neuropathic pain model. However, role of in inflammatory remains inconclusive. Therefore, we aimed to explore whether spinal cord dorsal root ganglion contributes complete Freund's adjuvant (CFA)-induced painful behaviors rats. Our results revealed that various biochemical morphological changes were associated with tissues CFA These included iron overload, enhanced lipid...

Perioperative neurocognitive disorder (PND) is a common complication in surgical patients. While many interventions to prevent PND have been studied, the availability of treatment methods limited. Thus, it crucial delve into mechanisms PND, pinpoint therapeutic targets, and develop effective approaches. In this study, reduced dorsal tenia tecta (DTT) neuronal activity was found be associated with tibial fracture surgery-induced indicating that excitation–inhibition (E–I) imbalance could...

Triocresyl phosphate (TOCP) was commonly used as flame retardant, plasticizer, lubricant, and jet fuel additive. Studies have shown adverse effects of TOCP on the reproductive system. However, potential harm brought by TOCP, especially to mammalian female cells, remains a mystery. In this study, we employed an in vitro model for first time investigate maturation process mouse oocytes. exposure hampered meiotic division process, evidenced reduction extrusion polar body from Subsequent...

Abstract α -Actinins play crucial roles in cytoskeletal mechanobiology by acting as force-bearing structural modules that orchestrate and sustain the framework, serving pivotal hubs for diverse mechanosensing proteins. The mechanical stability of -actinin dimer, a determinant its functional state, remains largely unexplored. Here, we directly quantify force-dependent lifetimes homo- hetero-dimers human -actinins, revealing an ultra-high dimers associated with > 100 seconds lifetime within...

Soybean mosaic virus (SMV) is a member of Potyvirus genus that causes severe yield loss and destroys seed quality in soybean [Glycine max (L.) Merr.]. It important to explore new resistance sources discover loci SMV, which will provide insights improve breeding strategies for SMV resistance. Here, genome-wide association study was conducted accelerate molecular the improvement soybean. A population 165 mutants derived from two parents used this study. There were 104 SNPs identified...

Abstract Objective Neural tube defects (NTDs) are the most serious and common birth in clinic. The SRY-related HMG box B1 (SoxB1) gene family has been implicated different processes of early embryogenesis. Sox19b is a maternally expressed SoxB1 that found region presumptive central nervous system (CNS), but its role mechanism embryonic neural stem cells (NSCs) during development have not yet explored. Considering specific to bony fish, we intended investigate zebrafish embryos. Material...

Abstract Offspring of mothers with hyperglycemia during pregnancy have a higher incidence long-term neuropsychiatric disorders than offspring from normal pregnancy, indicating that neocortical neurogenesis might be affected by maternal hyperglycemia. A paucity study evaluating the effects on neurogenetic differentiation neural stem cells, and mechanism remains unclear. We sought to investigate roles possible molecular cells. established mouse model hyperglycemic intrauterine exposure...

Hypoxic-ischemic encephalopathy (HIE) is one main cause of neonatal death and disability, causing substantial injury to white gray matter, which can lead severe neurobehavioral dysfunction, including intellectual disability dyskinesia. Inflammation, nerve cell death, matter are important factors in the pathological process HIE. 6-Gingerol a ginger extract, reduces inflammatory response death. However, role hypoxic-ischemic brain (HIBI) remains unknown. In this study, we constructed mouse...

Cognitive impairment is a complication that most frequently happens in patients with chronic neuropathic pain and has limited effective therapy. The aim of this study was to explore the effects curcumin on cognitive deficit rats peripheral nerve injury induced-neuropathic pain.The rat model constructed using constriction (CCI). (60 mg/kg) or vehicle intraperitoneally administered once day, beginning at 14th day after surgery continued for 14 consecutive days. nociceptive threshold tests were...