A5032327905- Protein Hydrolysis and Bioactive Peptides
- Food Quality and Safety Studies
- Probiotics and Fermented Foods
- Enzyme Production and Characterization
- Fermentation and Sensory Analysis
- Insect Utilization and Effects
- Marine Toxins and Detection Methods
- Microbial Metabolites in Food Biotechnology
- Biochemical effects in animals
- Tea Polyphenols and Effects
- Polysaccharides and Plant Cell Walls
- Computational Drug Discovery Methods
- Marine Biology and Environmental Chemistry
- Biofuel production and bioconversion
- Microbial Metabolism and Applications
- Genetics and Plant Breeding
- Spectroscopy and Chemometric Analyses
- Redox biology and oxidative stress
- Genomics, phytochemicals, and oxidative stress
- Treatment of Major Depression
- Venomous Animal Envenomation and Studies
- Antimicrobial Peptides and Activities
- Forensic Toxicology and Drug Analysis
- Food composition and properties
- Aquaculture disease management and microbiota
Xihua University
2021-2024
Fujian Fisheries Research Institute
2020-2024
Zhejiang Chinese Medical University
2024
China Jiliang University
2024
University of Otago
2019-2022
Jimei University
2021
Jiangnan University
2014
The First People's Hospital of Changzhou
2009
Liaoning University
2008
Kyushu Institute of Technology
1993
Β-glucan is a strongly hydrophilic non-starchy polysaccharide, which, when incorporated in food, renowned for its ability to alter functional characteristics such as viscosity, rheology, texture, and sensory properties of the food product. The β-glucans are directly linked their origin/source, molecular weight, structural features. weight structural/conformational features turn influenced by method extraction modification β-glucan. For example, whereas physical techniques influence only...
In this study, hypotensive peptides derived from mature flaxseed protein sequences were predicted in silico using BIOPEP-UWM with nine proteases, three each digestive, plant and microbial sources. The physicochemical properties of 2256 ACE-inhibitory 267 renin-inhibitory (including seven (7) dual inhibitory activities against both ACE renin enzymes) assessed the 'Peptides' package R. showed relatively low molecular weight (mol. wt.) range (132 = mol. wt. ≤ 442 Da); broad isoelectric point...
Protamine is a cationic peptide derived from fish sperm and has several important functional properties: antibacterial properties, acting as carrier for injectable insulin heparin antagonist, combatting fatigue, etc. Thus, it been widely used in medicinal applications food products. Cultured Takifugu flavidus type of pufferfish with delicious taste that popular China, its production increasing significantly. Therefore, protamine was extracted via acid extraction the further isolated purified...
Alcalase, neutral protease, and pepsin were used to hydrolyze the skin of
Broad bean fermentation is of vital importance in PixianDouban (PXDB) production, as well a key process for microorganisms to degrade protein, which lays the foundation formation PXDB flavor. In this study, two fungi and bacteria were screened, their morphology, molecular biology, growth, enzyme production characteristics analyzed, then they applied broad simulation system. The peptide, amino acid, nitrogen, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) system...
Angiotensin-I-converting enzyme (ACE) is a crucial or receptor that catalyzes the generation of potent vasopressor angiotensin II (Ang II). ACE inhibitory peptides from fish showed effective activity. In this study, we reported an peptide Takifugu bimaculatus (T. bimaculatus), which was obtained by molecular docking with acid-soluble collagen (ASC) hydrolysate T. bimaculatus. The antihypertensive effects and potential mechanism were conducted using Ang-II-induced human umbilical vein...
Abstract Phytohemagglutinin (PHA) is a seed storage protein and type of lectin originally discovered in the common bean (Phaseolus vulgaris) for its blood-agglutinating effect. Due to interactions with gut epithelia digestive enzymes potential trigger allergic reactions, PHA can lead various symptoms human body. As result, it has been regarded as significant antinutritional factor beans other legumes. While several published works have summarized structural, biochemical, toxicological...
The endoenzyme β-galactosidase (β-d-galactoside galactohydrolase; EC 3.2.1.23) has been used at industrial scales for the preparation of lactose-free milk and conversion lactose to galacto-oligosaccharides (GOS) prebiotics. In this study, using Plackett–Burman (PB) design response surface methodology (RSM), batch growth conditions production in DeMan-Rogosa-Sharpe (MRS) media have studied optimized Lactobacillus leichmannii 313 (ATCC 7830™) first time. incubation temperature (30 < T 55...
Candidate peptides with novel angiotensin-I-converting enzyme (ACE) inhibitor activity were obtained from hydrolysates of Gracilariopsis lemaneiformis by virtual screening method. Our results showed that G. (GLP) could significantly lower blood pressure in spontaneously hypertensive rats (SHR). At least 101 peptide sequences GLP identified LC-MS/MS analysis and subjected to screening. A total 20 the highest docking score selected chemically synthesized order verify their ACE-inhibitory...
Abstract Superoxide dismutase (SOD) is an acidic metalloenzyme that scavenges free radicals produced by endogenous and exogenous substances. In the present study, tissue distribution of superoxide HdhCu/Zn-SOD was investigated in Haliotis discus hannai Ino. The expression profile after lipopolysaccharide (LPS) challenge determined using quantitative real-time polymerase chain reaction (qPCR). To study antioxidant activity a recombinant protein, gene cloned into pPIC9K vector transformed...
The von Willebrand factor type D (VWD) domain in vitellogenin has recently been found to bind tetrodotoxin. way which this protein associates with tetrodotoxin and participates transporting vivo remains unclear. A cDNA fragment of the gene containing VWD from pufferfish (Takifugu flavidus) (TfVWD) was cloned. Using silico structural docking analyses predicted protein, we determined that key amino acids (namely, Val115, ASP116, Val117, Lys122) TfVWD mediate its binding tetrodotoxin, supported...
An affinity chromatography filler of CNBr-activated Sepharose 4B-immobilized ACE was used to purify ACE-inhibitory peptides from Takifugu flavidus protein hydrolysate (<1 kDa). Twenty-four with an average local confidence score (ALC) ≥ 80% bounded components (eluted by 1 M NaCl) were identified LC-MS/MS. Among them, a novel peptide, TLRFALHGME, activity (IC50 = 93.5 µmol·L-1) selected. Molecular docking revealed that TLRFALHGME may interact the active site through H-bond, hydrophobic, and...
Pixian Douban (PXDB) is one of the most popular condiments in China due to its unique flavor. Wheat flour that contains abundant nutrients an important raw material producing flavors during PXDB fermentation. In this study, wheat was substituted with soybean and gluten have higher proteins portions 10.4% 4.2% (F1), 8.9% 7.2% (F2), 9.6% 5.8% (F3). The results indicated substitutions increased amino acid nitrogen content improved flavor quality compared traditional group (CT). Especially, key...
The Nrf2/ARE pathway is considered the most important endogenous antioxidant signaling in mammals, playing a crucial role defending against external damage. This study investigated functional characteristics of Nrf2 abalone,
Abstract As an essential flavor condiment in Sichuan cuisine, Pixian Douban (PXDB) is usually produced by open fermentation process strip pools or ceramic vats. In this study, experiment of PXDB was conducted for 90 days a closed system gradient steady‐state temperature field (GSTF). To investigate the characterization volatile compounds system, volatiles three kinds samples including GSTF (SGT), constant (SCT), and traditional (STF) were analyzed. The results showed that 75, 67, 68 detected...
The drug-likeness and pharmacokinetic properties of 23 dairy-protein-derived opioid peptides were studied using SwissADME ADMETlab in silico tools. All the had poor drug-like based on violations Lipinski’s rule-of-five. Moreover, prediction their showed that intestinal absorption bioavailability. Following this, two well-known (βb-casomorphin-5, βb-casomorphin-7) from A1 bovine milk caffeine (positive control) selected for molecular docking vitro inhibition study with cholinesterase enzyme...
Pufferfish are considered a culinary delicacy but require careful preparation to avoid ingestion of the highly toxic tetrodotoxin (TTX), which accumulates in certain tissues. In this study, tissue distribution peroxiredoxin-1 from Takifugu bimaculatus was investigated. The protein obtained by vitro recombinant expression and purification. had strong ability scavenge hydroxyl radicals, protect superhelical DNA plasmids oxidative damage, L929 cells H2O2 toxicity through antioxidant activity....
Crude β-D-galactosidase from Lactobacillus leichmannii 313 was used to synthesise galacto-oligosaccharides (GOS) lactose. Using an initial lactose concentration of 170 g/L, the yield GOS 52 g/L (i.e. 62% conversion) after 2 h enzymatic reaction. The highest number unique individual were generated 48 ESI-MS and HPAEC analysis enriched fractions obtained mixtures showed presence sugars with degrees polymerization (DP) ranging 6. Further (by NMR spectroscopy) in fraction 3 (containing ~ 96% w/w...