A5072773886- Pluripotent Stem Cells Research
- Autonomous Vehicle Technology and Safety
- Software Reliability and Analysis Research
- RNA regulation and disease
- Anesthesia and Neurotoxicity Research
- Fluorine in Organic Chemistry
- Inorganic Fluorides and Related Compounds
- CRISPR and Genetic Engineering
- Safety Systems Engineering in Autonomy
- Neurogenesis and neuroplasticity mechanisms
- Epigenetics and DNA Methylation
- Power Systems Fault Detection
- Viral Infections and Immunology Research
- RNA Research and Splicing
- Formal Methods in Verification
- Nuclear Receptors and Signaling
- Embedded Systems Design Techniques
- Anomaly Detection Techniques and Applications
- 3D Printing in Biomedical Research
- Lightning and Electromagnetic Phenomena
- Usability and User Interface Design
- Pancreatic function and diabetes
- Real-Time Systems Scheduling
- Radiation Effects in Electronics
- Thermal Analysis in Power Transmission
University of Göttingen
2023-2024
TU Dortmund University
2021
Kettering University
2013-2019
Memorial Sloan Kettering Cancer Center
2018-2019
Vector (United States)
2018
New York Stem Cell Foundation
2016
University of Konstanz
2010-2014
Universidad de Navarra
2014
Osnabrück University
2013
Fraunhofer Institute for Experimental Software Engineering
2011-2012
Background: Information on the potential developmental toxicity (DT) of majority chemicals is scarce, and test capacities for further animal-based testing are limited. Therefore, new approaches with higher throughput required. A screening strategy based use relevant human cell types has been proposed by U.S. Environmental Protection Agency others. Because impaired neural crest (NC) function one known causes teratologic effects, toxicant effects NC cells desirable a DT battery.Objective: We...
Cranial placodes are embryonic structures essential for sensory and endocrine organ development. Human placode development has remained largely inaccessible despite the serious medical conditions caused by dysfunction of placode-derived tissues. Here, we demonstrate efficient derivation cranial from human pluripotent stem cells. Timed removal BMP inhibitor Noggin, a component dual-SMAD inhibition strategy neural induction, triggers induction at expense CNS fates. Concomitant fibroblast...
Exposure to the antiepileptic drug valproic acid (VPA) during gestation causes neurofunctional and anatomic deficits in later life. At present, there are little human data on how early neural development is affected by chemicals. We used embryonic stem cells, differentiating neuroectodermal precursors, as a model investigate modes of action VPA. Microarray expression profiling, qPCR specific marker genes, immunostaining green fluorescent protein under control promoter canonical precursor...
Developmental toxicity in vitro assays have hitherto been established as stand-alone systems, based on a limited number of toxicants. Within the embryonic stem cell-based novel alternative tests project, we developed test battery framework that allows inclusion any developmental assay and explores responses such systems to wide range drug-like compounds. We selected 28 compounds, including several biologics (e.g., erythropoietin), classical pharmaceuticals roflumilast) also six environmental...
Significance We previously demonstrated that induced pluripotent stem cell (iPSC)-derived cortical neurons from HSV-1 encephalitis patients with Toll-like receptor 3 (TLR3) pathway deficiencies are highly susceptible to HSV-1, due impairment of autonomous TLR3-IFN immunity. In this study we present a protocol for efficient derivation/purification trigeminal ganglion (TG) human iPSCs. The resulting TG sensory identity and exhibit robust biological function. also show play distinct roles in...
Human pluripotent stem cells (hPSCs) provide an unlimited cell source for regenerative medicine. Hormone-producing are particularly suitable therapy, and hypopituitarism, a defect in pituitary gland function, represents promising therapeutic target. Previous studies have derived lineages from mouse human ESCs using 3D organoid cultures that mimic the complex events underlying development vivo. Instead of relying on unknown cellular signals, we present simple efficient strategy to derive...
Perinatal exposure to low doses of methylmercury (MeHg) can cause adult neurological symptoms. Rather than leading a net cell loss, the toxicant is assumed alter differentiation and neuronal functions such as catecholaminergic transmission. We used neuronally differentiating murine embryonic stem cells (mESC) explore subtle toxicity. The mixed cultures that formed within 20 days contained small subpopulation tyrosine hydroxylase (TH)–positive neurons with specific dopaminergic dopamine...
Environmental and genetic risk factors contribute to Parkinson's Disease (PD) pathogenesis the associated midbrain dopamine (mDA) neuron loss. Here, we identify early PD pathogenic events by developing methodology that utilizes recent innovations in human pluripotent stem cells (hPSC) chemical sensors of HSP90-incorporating chaperome networks. We show triggered PD-related or toxic stimuli alter neuronal proteome, thereby altering stress-specific networks, which produce changes detected...
The cuprate complexes [Cu(R)(CF3 )3 ]- (R=organyl) offer an efficient synthetic access to valuable trifluoromethylation products RCF3 . Here, electrospray-ionization mass spectrometry is used analyze the formation of these intermediates in solution and probe their fragmentation pathways gas phase. Furthermore, potential energy surfaces systems are explored by quantum chemical calculations. Upon collisional activation, (R=Me, Et, Bu, s allyl) afford product ions [Cu(CF3 ]⋅- )2 former...
Abstract The directed generation of pure astrocyte cultures from pluripotent stem cells has proven difficult. Generation defined pluripotent‐stem‐cell derived astrocytes would allow new approaches to the investigation plasticity and heterogeneity astrocytes. We here describe a two‐step differentiation scheme resulting in murine embryonic cell (mESC) (MEDA), as characterized by upregulation 19 astrocyte‐associated mRNAs, positive staining most for GFAP (glial fibrillary acidic protein),...
We have recently been witnessing the growing establishment of map-centered web-based geo-portals on national, regional and local levels. However, a particular issue with these is that each instance has implemented in different ways terms design, usability, functionality, interaction possibilities, map size symbologies. In this paper, we try to tackle shortcomings by analyzing formalizing requirements for map-based user experience based approach. First, propose holistic definition term...
Epigenetic changes, including histone modifications or chromatin remodeling are regulated by a large number of human genes. We developed strategy to study the coordinate regulation such genes, and compare different cell populations tissues. A set 150 comprising classes epigenetic modifiers was compiled. This new tool used initially characterize changes during differentiation embryonic stem cells (hESC) central nervous system neuroectoderm progenitors (NEP). qPCR analysis showed that more...