A5048213038- Neurogenesis and neuroplasticity mechanisms
- Nerve injury and regeneration
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Glioma Diagnosis and Treatment
- Spinal Cord Injury Research
- Nuclear Receptors and Signaling
- Neuroinflammation and Neurodegeneration Mechanisms
- Mitochondrial Function and Pathology
- Neuroscience and Neural Engineering
- Cognitive Computing and Networks
- Graph Theory and Algorithms
- Mesenchymal stem cell research
- Molecular Biology Techniques and Applications
- Carcinogens and Genotoxicity Assessment
- Proteoglycans and glycosaminoglycans research
- Cancer-related cognitive impairment studies
- Brain Metastases and Treatment
- Congenital heart defects research
- Pancreatic function and diabetes
- Biomedical Ethics and Regulation
- RNA Interference and Gene Delivery
- Advanced Graph Neural Networks
- Recommender Systems and Techniques
Memorial Sloan Kettering Cancer Center
2011-2025
Kettering University
2013-2016
Karolinska Institutet
2005-2014
University of Wisconsin–Madison
2011-2012
Swedish Foundation for Strategic Research
2006-2007
Karolinska University Hospital
2005
Cranial placodes are embryonic structures essential for sensory and endocrine organ development. Human placode development has remained largely inaccessible despite the serious medical conditions caused by dysfunction of placode-derived tissues. Here, we demonstrate efficient derivation cranial from human pluripotent stem cells. Timed removal BMP inhibitor Noggin, a component dual-SMAD inhibition strategy neural induction, triggers induction at expense CNS fates. Concomitant fibroblast...
While clinical trials are ongoing using human pluripotent stem cell-derived midbrain dopamine (mDA) neuron precursor grafts in Parkinson's disease (PD), current protocols to derive mDA neurons remain suboptimal. In particular, the yield of TH+ after vivo grafting and expression some subtype-specific markers can be further improved. For example, characterization by single cell transcriptomics has yielded only a small proportion considerable fraction contaminating populations. Here we present...
Human pluripotent stem cells (hPSCs) provide an unlimited cell source for regenerative medicine. Hormone-producing are particularly suitable therapy, and hypopituitarism, a defect in pituitary gland function, represents promising therapeutic target. Previous studies have derived lineages from mouse human ESCs using 3D organoid cultures that mimic the complex events underlying development vivo. Instead of relying on unknown cellular signals, we present simple efficient strategy to derive...
Abstract In vitro expanded neural precursor cells (NPCs) may provide a stable source for cell therapy. search of the optimal spinal cord repair, we investigated influences gestational age, regional heterogeneity, and long‐term in propagation. The cellular content neurosphere cultures prior to after differentiation was studied by immunocytochemistry flow cytometry. Human forebrain NPCs deriving from first‐trimester tissue were cultured as neurospheres presence epidermal growth factor, basic...
Abstract Remyelination of chronically demyelinated axons in multiple sclerosis (MS) requires the recruitment endogenous cells or their replacement by transplanted, exogenous oligodendrocyte progenitor (OPCs). We have previously shown that an OPC line, CG4, preferentially migrates after transplantation toward focal areas inflammatory demyelination and axon loss created injection zymosan rat spinal cord. Here we show many transplanted CG4 had already migrated into lesion 1 day. demonstrate a...
With the enhanced use of chemotherapy and advent increased patient survival rates, there are an increasing number cancer survivors living with chemotherapy-induced cognitive impairment. A growing clinical studies have brought to light association agents like methotrexate in generating these neurological sequelae, although mechanisms remain unclear.Here, we a clinically relevant regimen several cycles leucovorin rescue develop model impairment, investigate vivo long-term (16 mo) impact...
Abstract Multiple sclerosis (MS) is an inflammatory demyelinating disease of the central nervous system. Exogenous cell replacement in MS lesions has been proposed as a means achieving remyelination when endogenous failed. However, ability exogenous cells to remyelinate axons presence inflammation remains uncertain. We have explored remyelinating capacity oligodendrocyte progenitor line CG‐4 transduced with GFP gene and transplanted adjacent zymosan‐induced focal demyelination model rat...
Complex networks pervade various real-world systems, from the natural environment to human societies. The essence of these is in their ability transition and evolve microscopic disorder-where network topology node dynamics intertwine-to a macroscopic order characterized by certain collective behaviors. Over past two decades, complex science has significantly enhanced our understanding statistical mechanics, structures, underlying networks. Despite advancements, there remain considerable...
Recommender system is one of the most important information services on today's Internet. Recently, graph neural networks have become new state-of-the-art approach to recommender systems. In this survey, we conduct a comprehensive review literature network-based We first introduce background and history development both systems networks. For systems, in general, there are four aspects for categorizing existing works: stage, scenario, objective, application. networks, methods consist two...
Radiation therapy (RT) to the brain remains a powerful tool in management of wide range cancers brain, head and neck regions metastases. It is also used prophylaxis treatment regimens for leukemias small cell cancers. There an increasing awareness significant irreversible long-term side effects associated with RT, including cognitive decline impairment motor coordination. Our team others have shown that depletion oligodendrocyte progenitors (OPC) demyelination are major pathological features...
Human pluripotent stem cells show considerable promise for applications in regenerative medicine, including the development of cell replacement paradigms treatment Parkinson’s disease. Protocols have been developed to generate authentic midbrain dopamine (mDA) neurons capable reversing dopamine-related deficits animal models However, generation mDA at clinical scale suitable human application remains an important challenge. Here, we present neuron derivation protocol based on a two-step WNT...