A5069017186- Cardiac, Anesthesia and Surgical Outcomes
- Medical Malpractice and Liability Issues
- Bariatric Surgery and Outcomes
- Photoacoustic and Ultrasonic Imaging
- Advanced Fluorescence Microscopy Techniques
- Optical Coherence Tomography Applications
- Pediatric Hepatobiliary Diseases and Treatments
- Esophageal and GI Pathology
- Body Contouring and Surgery
- Optical Coatings and Gratings
- Cell Image Analysis Techniques
- Optical Imaging and Spectroscopy Techniques
- Diet and metabolism studies
- Gallbladder and Bile Duct Disorders
- Gastroesophageal reflux and treatments
- Clinical practice guidelines implementation
- Intestinal and Peritoneal Adhesions
- Single-cell and spatial transcriptomics
- Advancements in Photolithography Techniques
- Near-Field Optical Microscopy
- Pancreatic and Hepatic Oncology Research
- Blood groups and transfusion
- Nanofabrication and Lithography Techniques
- Obesity and Health Practices
- Abdominal Surgery and Complications
Sutter Medical Center
2017-2024
Swiss Center for Electronics and Microtechnology (Switzerland)
2020-2024
École Polytechnique Fédérale de Lausanne
2006-2022
MRC Laboratory of Molecular Biology
2021
Case Western Reserve University
2019
University Hospitals Cleveland Medical Center
2019
Maine Medical Center
2019
McGill University Health Centre
2019
University of Goma
2019
Queen's University
2019
We have successfully developed both spontaneous and inductive cardiomyocyte differentiation of iPS cells reprogrammed from human foreskin fibroblasts. The morphologically resemble cardiomyocytes which can beat. RT-PCR immunostaining show that cardiac markers are expressed comparable to the pattern authentic embryonic stem cells, indicating existence immature mature differentiated cardiomyocytes. 5-Azacytidine greatly enhanced efficiency differentiation, whereas dimethylsulfoxide had no...
Genomic changes that occur in breast cancer during the course of disease have been informed by sequencing primary and metastatic tumor tissue. For patients with relapsed disease, evolution genome highlights importance using a recent sample for genomic profiling to guide clinical decision-making. Obtaining tissue biopsy can be challenging, analysis circulating DNA (ctDNA) from blood may provide minimally invasive alternative.
In this study, we demonstrate that BMSCs (bone marrow mesenchymal stem cells) can be successfully differentiated into type II alveolar epithelial cells in vitro under mimic pulmonary microenvironment. were co-cultured with MRC-5 modified SAGM (small airway growth medium). The BMSC-derived morphologically resemble human lung cells. They began to appear after 10 days co-culture and became dominant day 15. Correspondingly, SPC (surfactant protein C), a specific functional marker of cells, was...
We present a novel extended-focus optical coherence microscope (OCM) attaining 0.7 μm axial and 0.4 lateral resolution maintained over depth of 40 μm, while preserving the advantages Fourier domain OCM. Our system uses an ultra-broad spectrum from supercontinuum laser source. As spans near-infrared to visible wavelengths (240 nm in bandwidth), we call visOCM. The combination such broad with high-NA objective creates almost isotropic 3D submicron resolution. analyze imaging performance visOCM...
In recent years, three-dimensional mesoscopic imaging has gained significant importance in life sciences for fundamental studies at the whole-organ level. this manuscript, we present an optical projection tomography (OPT) method designed of intact mouse brain. The system features isotropic resolution ~50 µm and acquisition time four to eight minutes, using a 3-day optimized clearing protocol. Imaging brain autofluorescence 3D reveals details neuroanatomy, while use fluorescent labels...
The Rayleigh-Taylor instability of a thin viscous film coating the inside spherical substrate is studied. properties are investigated by performing linear optimal transient growth analysis. pattern formation further analyzed using nonlinear numerical simulations.
Alzheimer's disease (AD) is characterized by amyloidosis of brain tissues.This phenomenon studied with genetically-modified mouse models.We propose a method to quantify in whole 5xFAD brains, model AD.We use optical projection tomography (OPT) and random forest voxel classifier segment measure amyloid plaques.We validate our preliminary cross-sectional study, where we 6136 ± 1637, 8477 3438, 17267 4241 plaques (AVG SD) at 11, 17, 31 weeks.Overall, this can be used the evaluation new...
In this paper we show that using optical photolithography it's possible to obtain submicron features for periodic structures the Talbot effect. To use effect without need of an absolute distance measurement between mask and wafer integrate over several exposures varying distances. Here discuss salient 'integrated lithography'. Particularly, good contrasts excellent control illumination light is essential; MO Exposure Optics (MOEO) developed by SUSS MicroOptics (SMO). Finally 1μm 0.55μm...
In diabetes, pancreatic β-cells play a key role.These cells are clustered within structures called islets of Langerhans inside the pancreas and produce insulin, which is directly secreted into blood stream.The dense vascularization critical for maintaining proper regulation glucose homeostasis known to be affected from early stage diabetes.The deep localization these in abdominal cavity renders their vivo visualization challenging task.A fast label-free imaging method with high spatial...
Extended-focus optical coherence tomography (xf-OCT) is a variant of (OCT) wherein the illumination and/or detection modes are engineered to provide constant diffractionless lateral resolution over an extended depth field (typically 3 10× Rayleigh range). xf-OCT systems operating at 800 nm have been devised and used in past image brain structures high-resolution vivo, but limited ∼500 μm penetration due their short wavelength. Here we present system optimized deeper within cortex by using...
Functional magnetic resonance (fMRI) imaging is the current gold-standard in neuroimaging. fMRI exploits local changes blood oxygenation to map neuronal activity over entire brain. However, its spatial resolution currently limited a few hundreds of microns. Here we use extended-focus optical coherence microscopy (xfOCM) quantitatively measure flow velocity during functional hyperaemia at high spatio-temporal somatosensory cortex mice. As acquires depth slices simultaneously, measurements can...
The H2020 project PULSAR (Prototype for an Ultra Large Structure Assembly Robot) development objective is to create three demonstrators that will pave the way construction of large structures in orbit. study case considered assembly a segmented primary mirror next generation 35m space-based telescope. In frame this project, CSEM developing Single Mirror Tile (SMT) host positioning mechanism capable adjusting position hexagonal order compensate inaccuracies generated by robotic assembly. This...