A5049916145- Diabetes and associated disorders
- Pancreatic function and diabetes
- Immune Cell Function and Interaction
- Malaria Research and Control
- Liver Disease Diagnosis and Treatment
- Metabolism and Genetic Disorders
- Mosquito-borne diseases and control
- Cannabis and Cannabinoid Research
- Drug Transport and Resistance Mechanisms
- Diet, Metabolism, and Disease
- Diabetes Management and Research
- Porphyrin Metabolism and Disorders
- Diabetes Treatment and Management
- Optical Imaging and Spectroscopy Techniques
- Xenotransplantation and immune response
- T-cell and B-cell Immunology
- Pancreatitis Pathology and Treatment
- Optical Coherence Tomography Applications
- Multiple Sclerosis Research Studies
- Research on Leishmaniasis Studies
- Adipokines, Inflammation, and Metabolic Diseases
- Retinal Diseases and Treatments
- Abdominal vascular conditions and treatments
- Immune cells in cancer
- Alcoholism and Thiamine Deficiency
Lund University
2013-2024
Bernhard Nocht Institute for Tropical Medicine
2005-2020
University of Copenhagen
2009-2013
University of Freiburg
2009
Summary Plasmodium berghei is the causative agent of rodent malaria and widely used as a model system to study liver stage parasites. The entry P. sporozoites into hepatocytes has extensively been studied, but little known about parasite–host interaction during later developmental stages intracellular parasite. Growth parasite far beyond normal size host cell an important stress factor for infected cell. Cell trigger programmed death (apoptosis) we examined several apoptotic markers in...
Obesity is associated with glucose intolerance and insulin resistance closely linked to the increasing prevalence of type 2 diabetes. In mouse models diet-induced obesity (DIO) diabetes, an increased fat intake results in adipose tissue expansion secretion proinflammatory cytokines. The innate immune system not only plays a crucial role obesity-associated chronic low-grade inflammation but it also proposed play modulating energy metabolism. However, little known about how modulation...
Severe lung injury, responsible for up to 15% of mortality in acute necrotizing pancreatitis patients, is promoted by neutrophil (PMN) migration into the lung. We have previously demonstrated that pulmonary injury mediated PMN-derived matrix metalloproteinase-9 (MMP-9). This study was conducted evaluate ability broad-spectrum MMP inhibitor doxycycline prevent secondary pancreatitis.Eighteen rats were randomized three groups: severe (SAP), + (SAP+Dox) (30 mg/kg body mass) or control. Acute...
In recent years, three-dimensional mesoscopic imaging has gained significant importance in life sciences for fundamental studies at the whole-organ level. this manuscript, we present an optical projection tomography (OPT) method designed of intact mouse brain. The system features isotropic resolution ~50 µm and acquisition time four to eight minutes, using a 3-day optimized clearing protocol. Imaging brain autofluorescence 3D reveals details neuroanatomy, while use fluorescent labels...
Cysteine proteases mediate liberation of Plasmodium berghei merozoites from infected hepatocytes. In an attempt to identify the responsible parasite proteases, we screened genome P. for cysteine protease-encoding genes. RT-PCR analyses revealed that transcription four out five serine repeat antigen (PbSERA) genes was strongly upregulated in late liver stages briefly before parasitophorous vacuole membrane ruptured release into host cell cytoplasm, suggesting a role PbSERA these processes....
Parasite proteases play key roles in several fundamental steps of the Plasmodium life cycle, including haemoglobin degradation, host cell invasion and parasite egress. exit from infected cells appears to be mediated by a class papain-like cysteine called ‘serine repeat antigens’ (SERAs). A SERA subfamily, represented falciparum SERA5, contains an atypical active site serine residue instead catalytic cysteine. Members this SERAser subfamily are abundantly expressed asexual blood stages,...
Quinoline-3-carboxamides (Q substances) are small molecule compounds with anti-inflammatory properties. In this study, we used one of these substances, Paquinimod, to treat a novel model for chronic liver inflammation and fibrosis, the NOD-Inflammation Fibrosis (N-IF) mouse. We show that treatment N-IF mice significantly reduced resulted in regression even when was initiated after onset disease. The disease phenotype associated systemic decrease number activation disease-promoting transgenic...
Autoimmune diabetes is a consequence of immune-cell infiltration and destruction pancreatic β-cells in the islets Langerhans. We analyzed cellular composition insulitic lesions autoimmune-prone non-obese diabetic (NOD) mouse observed peak recruitment plasmacytoid dendritic cells (pDCs) to NOD around 8–9 weeks age. This coincides with increased spontaneous expression type-1-IFN response genes CpG1585 induced production IFN-α from islets. The transcription factor E2-2 specifically required for...
Sterile liver inflammation and fibrosis are associated with many disorders of different etiologies. Both type 1 2 inflammatory responses have been reported to contribute pathology. However, the mechanisms controlling balance between these largely unknown. Natural killer T (NKT) cells can be activated rapidly secrete cytokines chemokines both responses. As proteins accumulate in types sterile inflammation, we hypothesized that may play a role this pathological process. We found transgenic NKT...
Hormone secretion from pancreatic islets is essential for glucose homeostasis, and loss or dysfunction of islet cells a hallmark type 2 diabetes. Maf transcription factors are crucial establishing maintaining adult endocrine cell function. However, during pancreas development, MafB not only expressed in insulin- glucagon-producing cells, but also Neurog3+ progenitor suggesting additional functions differentiation formation. Here, we report that deficiency impairs β clustering formation,...
Here we characterize a new animal model that spontaneously develops chronic inflammation and fibrosis in multiple organs, the non-obese diabetic (N-IF) mouse. In liver, N-IF mouse displays particularly evident around portal tracts central veins accompanied with evidence of abnormal intrahepatic bile ducts. The extensive cellular infiltration consists mainly macrophages, granulocytes, eosinophils, mast cells. This inflammatory syndrome is mediated by transgenic population natural killer T...
Metabolic dysfunction-associated steatohepatitis (MASH) is a common but frequently unrecognized complication of obesity and type 2 diabetes. The association between these conditions multifaceted involves complex interactions metabolic, inflammatory, genetic factors. Here we assess the underlying structural molecular processes focusing on immunological phase MASH in nonobese inflammation fibrosis (NIF) mouse model compare it to human disease as well other murine models. Histopathology...
In diabetes, pancreatic β-cells play a key role.These cells are clustered within structures called islets of Langerhans inside the pancreas and produce insulin, which is directly secreted into blood stream.The dense vascularization critical for maintaining proper regulation glucose homeostasis known to be affected from early stage diabetes.The deep localization these in abdominal cavity renders their vivo visualization challenging task.A fast label-free imaging method with high spatial...
Abstract Aims/hypothesis Rapid and adequate islet revascularisation restoration of the islet–extracellular matrix (ECM) interaction are significant factors influencing survival function transplanted islets in individuals with type 1 diabetes. Because ECM encapsulating is degraded during isolation, understanding process engraftment after transplantation essential needs further investigation. Methods Here we apply a longitudinal high-resolution imaging approach to investigate dynamics...
The transitions between developmental stages are critical points in the Plasmodium life cycle. development of livers their mammalian hosts bridges malaria transmission and onset clinical symptoms elicited by red blood cell infection. egress parasites from liver must be a carefully orchestrated process to ensure successful switch stage Cysteine protease activity is known required for liver-stage egress, but crucial cysteine protease(s) remained unidentified. Here, we characterize member...
Multiple Sclerosis (MS) is a progressive autoimmune inflammatory and demyelinating disease of the central nervous system (CNS). T cells play key role in progression neuroinflammation MS also experimental encephalomyelitis (EAE) animal models for disease. A technology quantitative 3 dimensional (3D) spatial assessment inflammation this other CNS conditions much needed. Here we present procedure 3D global quantification development based on Optical Projection Tomography (OPT). Applying...
ABSTRACT In a routine study of vaginal smears postmenopausal women we had previously established the connection between somatic or psychological stress on one hand, and an increase in number superficial cells other. This phenomenon was further analyzed by rats Wistar strain. Artificial induced swimming tests. Only total proved adequate for our experiments. Stress produced cornified oophorectomized rats. failed to appear when animals were subsequently adrenalectomized. most instances...
Imaging beta cells is a key step towards understanding islet transplantation. Although different imaging platforms for the recording of cell biology have been developed and utilized in vivo, they are limited terms allowing single resolution continuous longitudinal recordings. Because transparency cornea, anterior chamber eye (ACE) mice well suited to study human mouse pancreatic biology. Here description how this approach can be used perform recordings grafting revascularization individual...
<title>Abstract</title> Metabolic dysfunction-associated steatohepatitis (MASH) is a common but frequently unrecognized complication of obesity and type 2 diabetes. The association between these conditions multifaceted involves complex interactions metabolic, inflammatory, genetic factors. Here we assess the underlying structural molecular processes focusing on immunological phase MASH in NIF mouse model compare it to human disease as well other murine models. Histopathology together with...
Abstract Glucagon-like peptide 1 (GLP-1) is produced by L cells in the small intestine response to ingested glucose and increases insulin release from pancreatic beta activation of its cognate receptor (GLP-1R). Stimulation this also contributes increased cell survival regeneration. We have found that Non Obese Diabetic (NOD) mice express significantly lower levels GLP-1R than C57BL/6 mice, leaving NOD with an impaired GLP-1 stimulation. The expression appears be caused accelerated...
Imaging beta cells is a key step towards understanding islet transplantation. Although different imaging platforms for the recording of cell biology have been developed and utilized in vivo, they are limited terms allowing single resolution continuous longitudinal recordings. Because transparency cornea, anterior chamber eye (ACE) mice well suited to study human mouse pancreatic biology. Here description how this approach can be used perform recordings grafting revascularization individual...