A5049937684- Advanced Fluorescence Microscopy Techniques
- Optical Coherence Tomography Applications
- Photoacoustic and Ultrasonic Imaging
- Spectroscopy Techniques in Biomedical and Chemical Research
- Structural Health Monitoring Techniques
- Ultrasonics and Acoustic Wave Propagation
- Advanced biosensing and bioanalysis techniques
- Plasmonic and Surface Plasmon Research
- Optical Imaging and Spectroscopy Techniques
- Cell Image Analysis Techniques
- Cellular Mechanics and Interactions
- RNA Interference and Gene Delivery
- Advanced Biosensing Techniques and Applications
- 3D Printing in Biomedical Research
- Biocrusts and Microbial Ecology
- Fatigue and fracture mechanics
- Near-Field Optical Microscopy
- Advanced X-ray Imaging Techniques
- Concrete Corrosion and Durability
- Digital Holography and Microscopy
- Nanocluster Synthesis and Applications
- Optical measurement and interference techniques
- Diamond and Carbon-based Materials Research
- Force Microscopy Techniques and Applications
- Lipid Membrane Structure and Behavior
Chevron (United States)
2024
Institut Fresnel
2021-2023
Centre National de la Recherche Scientifique
2021-2022
Centrale Marseille
2021-2022
École Polytechnique Fédérale de Lausanne
2014-2017
Laboratoire d'Optique Appliquée
2017
Charles Humbert 8
2017
Friedrich Schiller University Jena
2015
Virginia Tech
1998
There is a continuous demand for imaging probes offering excellent performance in various microscopy techniques comprehensive investigations of cellular processes by more than one technique. Fluorescent nanodiamond-gold nanoparticles (FND-Au) constitute new class "all-in-one" hybrid particles providing unique features multimodal including optical imaging, electron microscopy, and, and potentially even quantum sensing. Confocal coherence the FND-Au allow fast inside living cells via emission,...
An ideal nanofabrication method should allow the organization of nanoparticles and molecules with nanometric positional precision, stoichiometric control, well-defined orientation. The DNA origami technique has evolved into a highly versatile bottom-up methodology that fulfils almost all these features. It enables positioning even orientation asymmetrical along predefined directions. However, orienting individual been standing challenge. Here, we show how single molecules, namely, Cy5 Cy3...
We study experimentally the excitation of radially polarized conical surface plasmon polariton (SPP) in a fully metal-coated conically tapered M-profile fiber which works as "plasmonic tip" for scanning near-field optical microscope (SNOM). This structure extends Kretschmann configuration to geometry. In this plasmonic tip, waveguide mode, propagating inside fiber, resonantly excites SPP on metal surface, consequently gets confined at apex where field oscillates longitudinally along tip...
We present a novel extended-focus optical coherence microscope (OCM) attaining 0.7 μm axial and 0.4 lateral resolution maintained over depth of 40 μm, while preserving the advantages Fourier domain OCM. Our system uses an ultra-broad spectrum from supercontinuum laser source. As spans near-infrared to visible wavelengths (240 nm in bandwidth), we call visOCM. The combination such broad with high-NA objective creates almost isotropic 3D submicron resolution. analyze imaging performance visOCM...
In recent years, three-dimensional mesoscopic imaging has gained significant importance in life sciences for fundamental studies at the whole-organ level. this manuscript, we present an optical projection tomography (OPT) method designed of intact mouse brain. The system features isotropic resolution ~50 µm and acquisition time four to eight minutes, using a 3-day optimized clearing protocol. Imaging brain autofluorescence 3D reveals details neuroanatomy, while use fluorescent labels...
We report a bioinspired multifunctional albumin derived polypeptide coating comprising grafted poly(ethylene oxide) chains, multiple copies of the HIV TAT peptide enabling cellular uptake as well mitochondria targeting triphenyl-phosphonium (TPP) groups. Exploring these copolymers for passivating gold nanoparticles (Au NPs) yielded (i) NIR-emitting markers in confocal microscopy and (ii) photo-thermal active probes optical coherence microscopy. demonstrate great potential such...
Abstract We present a 3D time-lapse imaging method for monitoring mitochondrial dynamics in living HeLa cells based on photothermal optical coherence microscopy and using novel surface functionalization of gold nanoparticles. The biocompatible protein-based biopolymer coating contains multiple functional groups which impart better cellular uptake mitochondria targeting efficiency. high stability the nanoparticles allows continuous over an extended time up to 3000 seconds without significant...
We present a phase-shifting quantitative phase imaging technique providing high temporal and spatial stability acquisition speed. A piezoelectric microfabricated modulator allows tunable modulation frequencies up to the kHz range. After assessing accuracy with technical samples, we demonstrate rate while monitoring cellular processes at scales ranging from milliseconds hours.
Abstract A steel bridge hanger with three fatigue cracks was monitored for acoustic emission (AE) using combined source location, strain gauge monitoring, and waveform analysis. AE activities from all were clearly identified classified as crack growth or noise signals magnitude, position on cycle, uniqueness of waveforms the primary criteria. vast majority found to be due face rubbing crushing corrosion products between faces while limited emissions detected. Results laboratory tests A588...
Abstract A steel bridge hanger with three fatigue cracks was monitored for acoustic emission (AE) using combined source location, strain gauge monitoring, and waveform analysis. AE activities from all were clearly identified classified as crack growth or noise signals magnitude, position on cycle, uniqueness of waveforms the primary criteria. vast majority found to be due face rubbing crushing corrosion products between faces while limited emissions detected. Results laboratory tests A588...
Abstract In 2021, Tengizchevroil (TCO) initiated a pivotal standardization of business processes, data, and technology in several directions, particularly asset management materials line with Chevron’s enterprise program. Thus, digital solutions were aimed at boosting competitive performance, increasing reliability, preventing failures, managing risk minimizing the lifecycle cost surface assets. The central foundation for successful implementation these is high-quality data management,...
Studying the structural dynamics of lipid membranes requires methods that can address both microscopic and macroscopic characteristics. Fluorescence imaging is part most used techniques to study membrane properties in various systems from artificial cells: It benefits a high sensitivity local such as polarity molecular orientational order, with spatial resolution down single-molecule level. The influence embedded fluorescent probes on molecules however poorly known relies often simulations,...
We review optical coherence microscopy and present selected structural functional imaging applications ranging from diabetes to brain imaging, including new concepts for cell with an emphasis on the underlying concepts.
The photothermal optical lock-in coherence microscope (poli-OCM) introduced molecular specificity to OCM imaging, which is conventionally, a label-free technique. Here we achieve three-dimensional live cell and mitochondria specific imaging using ~4nm protein-functionalized gold nanoparticles (AuNPs). These do not photobleach demonstrate they're suitability for long-term time lapse imaging. We compare the accuracy of labelling with these AuNPs classical fluorescence confocal standard marker....
Mitochondrial dynamics refers to the processes of fusion, fission, and transport that aid mitochondria in accomplishing their many roles; including ATP production, oxygen sensing, homeostasis. Due involvement numerous essential cellular activity, dysfunctional have been implicated a wide range human diseases. Confocal microscopy using fluorophores for molecular specificity remains gold standard intracellular imaging. However, fluorescent labels can be toxic cell upon prolonged exposure still...
We present a novel extended-focus optical coherence microscope (OCM) attaining 0.7 μm axial and 0.4 lateral resolution maintained over depth of 40 μm, while preserving the advantages Fourier domain OCM. Our method uses an ultra-broad spectrum from super- continuum laser source. As spans near-infrared to visible wavelengths (240 nm in bandwidth), we call visOCM. The combination such broad with high-NA objective creates almost isotropic 3D submicron resolution. analyze imaging performance...
The mechanical actuation of cells by active forces from the cytoskeleton drives tissue morphogenesis. To understand these forces, multicellular laser dissection has become an essential tool for severing locally and inferring tension recoil surrounding structures. However, conventional is limited 2D steering, which inadequate embryos developing tissues that are intrinsically 3D In this study, we introduce a flexible near-infrared (NIR) fs-pulsed system allows trajectories to proceed in adapt...
Measuring single molecules' 3D orientations simultaneously with their spatial localization is currently a topic of intense efforts in optical design. We have developed different methods based on polarized imaging, that are capable to report both and orientation (including wobbling) from molecules. present how the design such schemes can be adapted account experimental constraints versus accuracy precision estimation parameters. illustrate performances actin structural STORM imaging complex...