A5048950940- Advanced Fluorescence Microscopy Techniques
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Near-Field Optical Microscopy
- Advanced Electron Microscopy Techniques and Applications
- Photosynthetic Processes and Mechanisms
- Microtubule and mitosis dynamics
- Plasmonic and Surface Plasmon Research
- Cell Image Analysis Techniques
- Optical measurement and interference techniques
- Advanced Materials Characterization Techniques
- Quantum-Dot Cellular Automata
- Electrostatics and Colloid Interactions
- Force Microscopy Techniques and Applications
- Neural dynamics and brain function
- Cellular transport and secretion
- Cellular Mechanics and Interactions
- Non-Destructive Testing Techniques
- Protist diversity and phylogeny
- Digital Holography and Microscopy
- Nanopore and Nanochannel Transport Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Fish Ecology and Management Studies
- Photoreceptor and optogenetics research
- Fuel Cells and Related Materials
Chan Zuckerberg Initiative (United States)
2024
Technical University of Denmark
2022-2024
Delft University of Technology
2016-2022
Microtubule (MT)-associated protein 7 (MAP7) is a required cofactor for kinesin-1-driven transport of intracellular cargoes. Using cryo-electron microscopy and single-molecule imaging, we investigated how MAP7 binds MTs facilitates kinesin-1 motility. The MT-binding domain (MTBD) bound as an extended α helix between the protofilament ridge site lateral contact. Unexpectedly, MTBD partially overlapped with binding inhibited its However, by tethering to MT, projection prevented dissociation...
Nanopores have become ubiquitous components of systems for single-molecule manipulation and detection, in particular DNA sequencing where electric field driven translocation through a nanopore is used to read out the molecule. Here, we present double-pore system two nanopores are drilled parallel same solid-state membrane, which offers new opportunities manipulation. Our experiments molecular dynamics simulations show that simultaneous electrophoretic capture molecule by mechanically traps...
An ideal nanofabrication method should allow the organization of nanoparticles and molecules with nanometric positional precision, stoichiometric control, well-defined orientation. The DNA origami technique has evolved into a highly versatile bottom-up methodology that fulfils almost all these features. It enables positioning even orientation asymmetrical along predefined directions. However, orienting individual been standing challenge. Here, we show how single molecules, namely, Cy5 Cy3...
Tracking live cells across 2D, 3D, and multi-channel time-lapse recordings is crucial for understanding tissue-scale biological processes. Despite advancements in imaging technology, achieving accurate cell tracking remains challenging, particularly complex crowded tissues where segmentation often ambiguous. We present Ultrack, a versatile scalable cell-tracking method that tackles this challenge by considering candidate segmentations derived from multiple algorithms parameter sets. Ultrack...
Abstract Particle fusion for single molecule localization microscopy improves signal-to-noise ratio and overcomes underlabeling, but ignores structural heterogeneity or conformational variability. We present a-priori knowledge-free unsupervised classification of structurally different particles employing the Bhattacharya cost function as dissimilarity metric. achieve 96% accuracy on mixtures up to four DNA-origami structures, detect rare classes origami occuring at 2% rate, capture variation...
We present a fast particle fusion method for particles imaged with single-molecule localization microscopy. The state-of-the-art approach based on all-to-all registration has proven to work well but its computational cost scales unfavorably the number of N, namely as N2. Our overcomes this problem and achieves linear scaling N by making use Joint Registration Multiple Point Clouds (JRMPC) method. Straightforward application JRMPC fails mostly locally optimal solutions are found. These...
Nanoparticles (NPs) have proven their applicability in biosensing, drug delivery, and photothermal therapy, but performance depends critically on the distribution number of functional groups surface. When studying surface functionalization using super-resolution microscopy, NP modifies fluorophore's point-spread function (PSF). This leads to systematic mislocalizations conventional analyses employing Gaussian PSFs. Here, we address this shortcoming by deriving analytical PSF model for a...
SUMMARY Microtubule (MT)-associated proteins (MAPs) regulate intracellular transport by selectively recruiting or excluding kinesin and dynein motors from MTs. We used single-molecule cryo-electron imaging to determine the mechanism of MAP-motor interactions in vitro. Unexpectedly, we found that regulatory role a MAP cannot be predicted based on whether it overlaps with motor binding site forms liquid condensates MT. Although MT domain (MTBD) MAP7 kinesin-1 site, tethering projection...
Abstract Voltage imaging and optogenetics offer new routes to optically detect influence neural dynamics. Optimized hardware is necessary make the most of these techniques. Here we present Octoscope, a versatile, multimodal device for all-optical electrophysiology. We illustrate its concept design demonstrate capability perform both 1-photon 2-photon voltage with spatial temporal light patterning, in inverted upright configurations, vitro vivo .
Controlling the flow of excitons between organic molecules holds immense promise for various applications, including energy conversion, spectroscopy, photocatalysis, sensing, and microscopy. DNA nanotechnology has shown in achieving this control by using synthetic as a platform positioning and, very recently, also orienting dyes. In study, orientation doubly-linked dyes origami structures was manipulated to transfer. By controlling independently single donor acceptor molecules, average...
We introduce inTRACKtive, an innovative web-based tool for interactive visualization and sharing of large 3D cell tracking datasets, eliminating the need software installations or data downloads. Built with modern web technologies, inTRACKtive enables researchers to explore cell-tracking results from terabyte-scale microscopy data, conduct virtual fate-mapping experiments, share these via simple hyperlinks. The platform powers Virtual Embryo Zoo, online resource showcasing datasets...
Nanoparticles (NPs) have proven their applicability in biosensing, drug delivery, and photo-thermal therapy, but performance depends critically on the distribution number of functional groups surface. When studying surface functionalization using super-resolution microscopy, NP modifies fluorophores point-spread function (PSF). This leads to systematic mislocalizations conventional analyses employing Gaussian PSFs. Here, we address this shortcoming by deriving first-ever analytical PSF model...
An ideal nanofabrication method should allow the organization of nanoparticles and molecules with nanometric positional precision, stoichiometric control well-defined orientation. The DNA origami technique has evolved into a highly versatile bottom-up methodology that fulfils almost all these features. It enables positioning control, even orientation asymmetrical along predefined directions. However, orienting individual been standing challenge, mainly due to unspecific electrostatic...
Abstract We present a fast particle fusion method for particles imaged with single-molecule localization microscopy. The state-of-the-art approach based on all-to-all registration has proven to work well but its computational cost scales unfavourably the number of N , namely as 2 . Our overcomes this problem and achieves linear scaling by making use Joint Registration Multiple Point Clouds (JRMPC) method. Straightforward application JRMPC fails mostly locally optimal solutions are found....