A5100659046- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Congenital Heart Disease Studies
- RNA modifications and cancer
- Aortic Disease and Treatment Approaches
- Circular RNAs in diseases
- Cancer, Hypoxia, and Metabolism
- Coronary Artery Anomalies
- Cardiac Valve Diseases and Treatments
- Vascular anomalies and interventions
- Lung Cancer Treatments and Mutations
- Ubiquitin and proteasome pathways
- Ferroptosis and cancer prognosis
- Cancer-related Molecular Pathways
- Epigenetics and DNA Methylation
- Mesenchymal stem cell research
- Angiogenesis and VEGF in Cancer
- Flavonoids in Medical Research
- Autophagy in Disease and Therapy
- Cancer, Lipids, and Metabolism
- Cardiac and Coronary Surgery Techniques
- Chemokine receptors and signaling
- Caveolin-1 and cellular processes
- 14-3-3 protein interactions
- Cerebrovascular and Carotid Artery Diseases
Shanghai Jiao Tong University
2014-2024
XinHua Hospital
2014-2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2024
MicroRNAs (miRNAs) play critical roles in cancer development and progression. In this study, we examined the molecular mechanisms of miR-342-3p human non-small cell lung (NSCLC). The results showed that is downregulated NSCLC lines tissues, its overexpression induces significant inhibition proliferation, invasion, tumor growth nude mice. addition, repressed RAP2B expression through interactions with 3′-UTR region. Restoration reversed miR-342-3p-mediated inhibitory activity cells. Finally,...
Deubiquitinases (DUBs) and noncoding RNAs have been the subjects of recent extensive studies regarding their roles in lung cancer, but mechanisms involved are largely unknown. In our study, we used The Cancer Genome Atlas data set bioinformatics analyses identified USP21, a DUB, as potential contributor to oncogenesis non-small-cell cancer (NSCLC). We further demonstrated that USP21 was highly expressed NSCLCs. then conducted series vitro vivo assays explore effect on NSCLC progression...
Abstract Ferroptosis is a novel form of programmed cell death triggered by iron‐dependent lipid peroxidation and has been associated with various diseases, including cancer. Erastin, an inhibitor system Xc‐, which plays critical role in regulating ferroptosis, identified as inducer ferroptosis cancer cells. In this study, we investigated the impact butyrate, short‐chain fatty acid produced gut microbiota, on erastin‐induced lung Our results demonstrated that butyrate significantly enhanced...
In this study, we demonstrate that BMSCs (bone marrow mesenchymal stem cells) can be successfully differentiated into type II alveolar epithelial cells in vitro under mimic pulmonary microenvironment. were co-cultured with MRC-5 modified SAGM (small airway growth medium). The BMSC-derived morphologically resemble human lung cells. They began to appear after 10 days co-culture and became dominant day 15. Correspondingly, SPC (surfactant protein C), a specific functional marker of cells, was...
DDP (cisplatin) resistance in lung cancer has been widely reported. Baicalin is a flavone glycoside found genus Scutellaria. However, the effects of baicalin on are unclear. The aim present study was to investigate combination and proliferation invasion human cells, explore possible mechanisms. MTT assay utilized evaluate A549 A549/DPP (DPP-resistant) cells. probability sum method used determine drug combination. Transwell detect tumor cell invasion. mRNA expression MARK2 cells detected by...
Pericardial adipose tissue, which comprises both epicardial tissue (EAT) and paracardial (PAT), has recently been recognized as a novel factor in the pathophysiology of cardiovascular diseases, especially coronary artery disease (CAD). The goal this study was to evaluate differences brown-like characteristic proteome among human EAT, PAT, subcutaneous (SAT) identify candidate molecules causing CAD. Uncoupling protein 1 (UCP-1) other brown-related proteins were highly expressed pericardial...
Accumulating studies suggest that circular RNAs (circRNAs) function as key regulators in human cancers.We found hsa_circ_0001869 participated non-small cell lung cancer (NSCLC) progression.However, its expression and during NSCLC remain unknown.The data advised was increased lines tissues.High had negatively correlation with the patients prognosis.Bioinformatics luciferase report analyses confirmed miR-638 FOSL2 were downstream target.hsa_circ_0001869 downregulation decreased tumor...
Cancer stem cells (CSCs) are considered key contributors to tumor progression, and ferroptosis has been identified as a potential target for CSCs. We have previously shown that butyrate enhances the induced by erastin in lung cancer cell, this study aimed investigate impact of on progression To these effects, we constructed series vitro experiments, including 3D non-adherent sphere-formation, cytometry analysis, assessment CSC marker expression, cell migration assay, vivo tumorigenesis...
Abstract Background Gene targeted therapy refers to any focused on one of the many biological features tumor. Such are mediated by specific genes that involved in tumor metastasis, recurrence, poor response chemotherapy and others. Hypoxia is an important pathognomonic feature malignant tumors including SCLC (small cell lung cancer). HIF-1alpha, which induced hypoxia, most regulatory factor can influence tumors. Methods In this study, we tried elucidate changes gene expression profiles...
Increasing evidence suggests that ubiquitin-specific protease 22 (USP22) has great clinicopathologic significance in oncology. In this study, we investigated the role of USP22 human NSCLC tumorigenesis along with underlying mechanisms action. First, determined expression NSCLC, as well normal tissues and cell lines. We then studied effects silencing by shRNA on growth vitro vivo, effect p53 pathway. found is overexpressed inhibits proliferation, induces apoptosis arrests cells at G0/G1...
Non-small cell lung cancer (NSCLC) is one of the leading causes mortality worldwide. The role miR-26 in development and progression NSCLC remains largely unknown. In this study we found abnormal expression human tissues. It was that mimics induced apoptosis promoted caspase-3, 9 activities cells. inhibitor enhanced light chain 3 (LC3) protein autophagy related genes Moreover, regulated by inhibiting TGF-β a JNK dependent manner. addition, involved endoplasmic reticulum stress (ERS) signaling...
BACKGROUND: Matrix-remodeling associated 5 (MXRA5) has recently been one of the frequently mutated gene but its role in non-small cell lung cancer (NSCLC) remains unclear. OBJECTIVE: To identify whether MXRA5 could be applied as a novel prognostic marker for NSCLC. METHODS AND RESULTS: In this stu dy, we proved that mRNA and protein expression 166 NSCLC patients' tissues by Quantitative Real-Time PCR immunohistochemical staining respectively. Moreover, correlation between clinicopathological...
The previous study has shown that transcriptional factor MEOX1 could promote proliferation and sphere formation ability of non-small cell lung cancer (NSCLC) cells, however, we found mRNA was lowly expressed in tissues compared to normal adjacent tissues, expression positively correlated with the survival patients, especially adenocarcinoma patients. Functional experiments using vitro vivo revealed stable overexpression significantly suppressed ability, promoted cycle arrest G2 phase,...
Abstract Congenital quadricuspid aortic valve is a rare cardiac anomaly. More than half of the patients with this abnormality will develop insufficiency in adulthood. It vital that who present progressive regurgitation undergo replacement or repair at appropriate time. Here, we two cases valve. We first describe 58-year-old man had mitral and ascending aorta dilatation He underwent plasty recovered well. The second patient 20-year-old asymptomatic boy has been closely followed up not...
Activated cdc42-associated tyrosine kinase 1 (ACK1) has been reported to be implicated in non-small-cell lung cancer (NSCLC). However, the expression pattern and biological functions of ACK1 progression NSCLC are not fully understood. In this study, it was found that significantly up-regulated samples compared their adjacent normal tissues. Meanwhile, inversely correlated with survival patients. Moreover, function studies, further validated promote growth, migration, metastasis cells vitro...
Dysregulation of hedgehog signaling has been involved in esophageal squamous cell carcinoma (ESCC) by the mechanisms that are not fully understood. The receptor for activated protein kinase C (RACK1) is progression multiple cancers. However, its expression and function ESCC have investigated. Here, we found RACK1 was upregulated clinical samples. Moreover, over-expression cells promoted proliferation migration, while downregulation impaired migration vitro vivo. Mechanistically, activating...
Changes in pulmonary microvascular permeability following cardiopulmonary bypass (CPB) and the underlying mechanisms have not yet been established. Therefore, aim of present study was to elucidate alterations CPB mechanism. The measured using Evans Blue dye (EBD) exclusion, neutrophil infiltration proinflammatory cytokine secretion investigated. In addition, activation Src kinase phosphorylation caveolin‑1 vascular endothelial cadherin (VE‑cadherin) examined. results revealed that increased...
Endothelial progenitor cell (EPC) dysfunction is an important physiopathological mechanism in the dynamics of formation atherosclerosis. It has been reported that angiotensin II (Ang-II) damages function EPCs atherosclerotic plaque through induction oxidative stress. Sestrin 2 (Sesn2) serves as antioxidant role stress, however, exact mechanisms underlying how Sesn2 may factor into after Ang-II treatments needs to be illustrated. We isolated from human umbilical cord blood samples and treated...