Abstract Small extracellular vesicles (EVs), including exosomes, play multiple physiological roles. In neurodegenerative diseases, EVs can be pivotal in dispersing neuropathogenic proteins. This study investigates the role of neural stem cell (NSC)‐derived a transgenic (Tg) mouse model Alzheimer's disease (AD). Five weeks following treatment on 9‐month‐old APP/PS1 mice, effects NSC‐derived cognitive behavior, mitochondrial function, sirtuin1 (SIRT1), synaptic function and morphology,...

Background and Aims Flooding imposes stress upon terrestrial plants because it results in oxygen deficiency, which is considered a major problem for submerged plants. A common response of to flooding the formation aquatic adventitious roots. Some studies have shown that roots on are capable absorbing water nutrients. However, there no experimental evidence possible uptake function or how important this might be survival during prolonged submergence. This study aims investigate whether absorb...

Resistance to drug therapy is a major impediment for successful treatment of patients suffering from breast cancer (BC). Tamoxifen (TAM) an extensively used therapeutic agent, which substantially reduces the risk recurrence and associated mortality in BC. This study demonstrated that exosomal transfer microRNA-9-5p (miR-9-5p) enhanced resistance MCF-7 cells TAM.Initially, BC-related differentially expressed genes (DEGs) their upstream regulatory miRNAs were identified. The TAM-resistant...

Abstract Messenger RNA m 6 A modification is shown to regulate local translation in axons. However, how the codes axonal mRNAs are read and decoded by reader proteins still unknown. Here, it found that readers YTHDF1 YTHDF2 both expressed cerebellar granule cells (GCs) their Knockdown (KD) of or significantly increases GC axon growth rates vitro. By integrating anti‐YTHDF1&2 RIP‐Seq with quantitative proteomic analysis RNA‐seq after KD YTHDF2, a group transcripts which may mediate...

APP misexpression plays a crucial role in triggering complex pathological cascade, leading to Alzheimer's disease (AD). But how the expression of is regulated conditions remains poorly understood. In this study, we found that exosomes isolated from AD mouse brain promoted neuronal N2a cells. Moreover, derived cells with ectopic (APP-EXO) also induced dysregulation normal Surprisingly, effects APP-EXO on recipient were not mediated by direct transferring gene products. Instead, highly likely...

Abstract JOURNAL/nrgr/04.03/01300535-202407000-00040/figure1/v/2023-11-20T171125Z/r/image-tiff Mitochondrial dysfunction is a hallmark of Alzheimer's disease. We previously showed that neural stem cell-derived extracellular vesicles improved mitochondrial function in the cortex APP/PS1 mice. Because disease affects entire brain, further research needed to elucidate alterations metabolism brain as whole. Here, we investigated expression several important biogenesis-related cytokines multiple...

Myoblast fusion is essential to skeletal muscle development and repair. We have demonstrated previously that human myoblasts hyperpolarize, before fusion, through the sequential expression of two K+ channels: an ether-à-go-go inward rectifier. This hyperpolarization a prerequisite for as it sets resting membrane potential in range at which Ca2+ can enter thereby trigger via window current α1H T channels.

Two early signs of human myoblast commitment to fusion are membrane potential hyperpolarization and concomitant expression a non‐inactivating delayed rectifier K + current, I K(NI) . This current closely resembles the outward elicited by rat ether‐à‐go‐go (r‐ eag ) channels in its range for activation unitary conductance. It is shown that kinetics , like those r‐ depend on holding [Mg 2+ ] o reversibly inhibited rise [Ca ]. Forced an isolated channel (h‐ cDNA undifferentiated myoblasts...

Cerebral amyloid angiopathy (CAA) is present in up to 90% of patients with Alzheimer's disease (AD), and may interact classical neuropathology exacerbate cognitive decline. Since growth differentiation factor 11 (GDF11) can activate vascular remodeling, we tested its effects on function neuroinflammatory changes AD model mice. We intravenously administered GDF11 or vehicle daily 12-month-old transgenic mice overexpressing the amyloid-β protein precursor (AβPP)/PS1). Cognitive was monitored...

Extracellular vesicles (EVs) are important in the intercellular communication of central nervous system, and their release is increased during neuroinflammation. Our previous data demonstrated an EVs HIV-1 infection immune activation glial cells. However, molecular mechanism by which inflammation increase EV remains unknown. In current study, we investigated role glutaminase 1 (GLS1)-mediated glutaminolysis production a key metabolic intermediate α-ketoglutarate on release. Human...

Accumulating studies suggest that circular RNAs (circRNAs) function as key regulators in human cancers.We found hsa_circ_0001869 participated non-small cell lung cancer (NSCLC) progression.However, its expression and during NSCLC remain unknown.The data advised was increased lines tissues.High had negatively correlation with the patients prognosis.Bioinformatics luciferase report analyses confirmed miR-638 FOSL2 were downstream target.hsa_circ_0001869 downregulation decreased tumor...

The methylotrophic yeast

Abstract Prostate cancer is a common male with high morbidity and mortality worldwide. According to current research, the integration of long non-coding RNA (lncRNAs) microRNA(miRNAs) can be expressed in variety cancers play an important role diagnosis. Based on this, this study explored clinical lncRNA MAGI2-AS3 (MAGI2-AS3) prostate cancer. By detecting expression levels miR-142-3p, correlation between characteristics data was analyzed. ROC curve analysis performed area under (AUC) used...

Context: Previous studies have reported that pregnant women with non-alcoholic fatty liver disease (NAFLD) face an increased risk of gestational hypertension (GH) and preeclampsia (PE). However, no study has assessed the relationship between Hepatic Steatosis Index (HSI), a biomarker for NAFLD, in early pregnancy subsequent GH PE. Objective: We aimed to investigate HSI risks PE Chinese women. Methods: Based on China Birth Cohort Study conducted from February 2018 December 2022, this...

Diabetic retinopathy (DR) is a major cause of vision loss worldwide. Here, we perform single-cell RNA sequencing thirteen human retina samples (from living and post-mortem donors) across non-diabetic, diabetic, DR states to create comprehensive transcriptomic atlas. We uncover three distinct microglial states: homeostatic, stress-response, inflammatory, along functional continuum, rather than discrete activation states, with dynamic transitions occurring throughout disease progression....

Sleep disorders (SDs) are a common issue in the elderly. Epigenetic clocks based on DNA methylation (DNAm) now considered highly accurate predictors of aging process and associated with age-related diseases. This study aimed to investigate causal relationship between sleep traits epigenetic clock using Mendelian randomization (MR) analysis. The genome-wide association (GWAS) statistics for (HannumAge, intrinsic age acceleration [IEAA], PhenoAge, GrimAge) were obtained from UK Biobank (UKB),...

The human retina exhibits complex cellular heterogeneity which is critical for visual function, yet comprehensive ethnic-specific references are scarce in ophthalmic transcriptomics. lack of single-cell RNA sequencing (scRNA-seq) data from Asian populations particularly Chinese donors imposes significant limitations understanding population-specific retinal biology. We constructed the first transcriptomic atlas donors, generated through high-throughput scRNA-seq ~290,000 viable cells...

Objective: The present study aims to elucidate the significance of immune cell infiltration in Coronavirus disease 2019 (COVID-19) myocarditis and identify potential diagnostic markers for this condition. Myocarditis, an inflammatory cardiac disease, primarily results from viral infections. Although association between COVID-19 is well-established, specific mechanism(s) underlying relationship remain incompletely understood. Methods: GSE53607 GSE35182 datasets were obtained GEO database,...

Single-cell RNA sequencing (scRNA-seq) has significantly advanced our understanding of retinal aging, yet the specific molecular characteristics within cell populations remain incompletely defined. We profiled 223,612 single cells from 18 unfrozen human retinas obtained 13 Chinese donors aged 34-92 years, providing an ethnically atlas across adult lifespan. A sparsity-driven machine-learning (ML) pipeline (L1-regularized logistic regression, recursive feature elimination with...

Mapping the proteomic landscape of mitochondria with spatiotemporal precision plays a pivotal role in elucidating delicate biological functions and complex relationship other organelles variety dynamic physiological processes which necessitates efficient controllable chemical tools. We herein report photo-oxidation driven proximity labeling strategy to profile mitochondrial proteome by light dependence living cells high resolution. Taking advantage organelle-localizable organic...

Intestinal inflammation causes tight junction changes and death of epithelial cells, plays an important role in the development Crohn's disease (CD). CD52 monoclonal antibody (CD52 mAb) directly targets cell surface is effective depleting mature lymphocytes by cytolytic effects vivo, leading to long-lasting adaptive immunity. The aim this study was investigate therapeutic effect mAb on barrier function animal models IBD. Interleukin-10 knockout mice (IL-10(-/-) ) 16 weeks with established...

Background: Depression has a significant effect on cardiovascular disease (CVD), but uncertainties persist regarding which modifiable risk factors mediate the causal effects. We aim to determine whether depression is causally linked CVD and play potential mediating roles. Methods: used two-sample Mendelian randomization (MR) approach NHANES 2007–2018 data estimate effects of various cases investigated 28 mediators association between CVD. Results: The results our MR analysis indicated that...