A5017750524- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Metabolomics and Mass Spectrometry Studies
- Ionic liquids properties and applications
- Advanced Nanomaterials in Catalysis
- Angiogenesis and VEGF in Cancer
- Cancer, Hypoxia, and Metabolism
- Biotin and Related Studies
- Monoclonal and Polyclonal Antibodies Research
- Click Chemistry and Applications
- Advanced Biosensing Techniques and Applications
- Conducting polymers and applications
- RNA and protein synthesis mechanisms
- Zebrafish Biomedical Research Applications
- Cancer, Stress, Anesthesia, and Immune Response
- Cancer Cells and Metastasis
- Cell Adhesion Molecules Research
- Nanocluster Synthesis and Applications
- Organic Electronics and Photovoltaics
- Receptor Mechanisms and Signaling
- Protein purification and stability
- Axon Guidance and Neuronal Signaling
- Epigenetics and DNA Methylation
- Spinal Cord Injury Research
Dalian Institute of Chemical Physics
2016-2025
China Medical University
2022-2025
Chinese Academy of Sciences
2016-2025
Jinan University
2025
Chengdu University of Traditional Chinese Medicine
2025
University of Chinese Academy of Sciences
2021-2024
Jinzhou Medical University
2023-2024
Dalian University
2016-2020
Medical College of Wisconsin
2010-2018
Children's Hospital of Wisconsin
2010-2018
Chemical crosslinking coupled with mass spectrometry (CXMS) has emerged as a powerful technique to obtain the dynamic conformations and interaction interfaces of protein complexes. Limited by poor cell membrane permeability, chemical reactivity, biocompatibility crosslinkers, in vivo capture dynamics complexes finer temporal resolution higher coverage is attractive but challenging. In this work, trifunctional crosslinker bis(succinimidyl) propargyl tag (BSP), involving compact size, proper...
Rationale: Increased lipid droplet (LD) formation has been linked to tumor metastasis, stemness, and chemoresistance in various types of cancer. Here, we revealed that LD is critical for the adaptation sorafenib hepatocellular carcinoma (HCC) cells. We aim investigate function its regulatory mechanisms HCC. Methods: The key proteins responsible were screened by both metabolomics proteomics sorafenib-resistant HCC cells further validated immunoblotting immunofluorescence staining. Biological...
Abstract Protein N-phosphorylation plays a critical role in central metabolism and two/multicomponent signaling of prokaryotes. However, the current enrichment methods for O-phosphopeptides are not preferred N-phosphopeptides due to intrinsic lability P-N bond under acidic conditions. Therefore, effective N-phosphoproteome analysis remains challenging. Herein, bis(zinc(II)-dipicolylamine)-functionalized sub-2 μm core-shell silica microspheres (SiO 2 @DpaZn) tailored rapid enrichment. Due...
The selective and highly efficient capture of circulating tumor cells (CTCs) from blood their subsequent release without damage are very important for the early diagnosis tumors understanding mechanism metastasis. Herein, a universal strategy is proposed fabrication an antibody-free hydrogel that has synergistic effect by featuring microinterfaces obtained cell imprinting molecular recognition conferred boronate affinity. With this artificial antibody, human hepatocarcinoma SMMC-7721...
Recombinant protein technology occupies an important position in fields including biopharmaceutics, proteomics, structural and functional biology. However, the purification of His-tagged protein, majority portion recombinant is seriously hindered by impurities. These impurities, host proteins with inherent cysteine histidine-rich regions or metal centers, are usually beyond ability commonly used IMAC materials. To remove this barrier, a novel material was developed through enhancing...
Membrane channel proteins (MCPs) play key roles in matter transport through cell membranes and act as major targets for vaccines drugs. For emerging ionic liquid (IL) drugs, a rational understanding of how ILs affect the structure function MCP is crucial to their design. In this work, GPU-accelerated microsecond-long molecular dynamics simulations were employed investigate modulating mechanism on MCP. Interestingly, prefer insert into lipid bilayer aquaporin-2 (AQP2) but adsorb entrance...
NgBR is a type I receptor with single transmembrane domain and was identified as specific for Nogo-B. Our recent findings demonstrated that binds farnesylated Ras recruits to the plasma membrane, which critical step required activation of signaling in human breast cancer cells tumorigenesis. Here, we first use immunohistochemistry real-time PCR approaches examine expression patterns Nogo-B both normal tumor tissues. Then, relationship between molecular subtypes cancer, roles...
// Chengyong Dong 1, * , Baofeng Zhao 2, 6, 7, Fei Long 1 Ying Liu 3, 4 Zhenzhen Song Li Xuejun Yang Deguang Sun Haibo Wang Qinlong Rui Liang Yan Zhenming Gao Shujuan Shao 5 Qing Robert Miao 8 Liming Division of Hepatobiliary and Pancreatic Surgery, Department General The Second Affiiated Hospital Dalian Medical University, Dalian, Liaoning, China 2 Key Laboratory Separation Science for Analytical Chemistry, National Chromatographic R & A Center, Institute Chemical Physics, Chinese Academy...
Nogo‐B receptor (NgBR) was identified as a specific for binding and is essential the stability of Niemann‐Pick type C2 protein (NPC2) NPC2‐dependent cholesterol trafficking. Here, we report that NgBR expression levels decrease in fatty liver plays previously unrecognized roles regulating hepatic lipogenesis through NPC2‐independent pathways. To further elucidate pathophysiological role mammals, generated liver‐specific knockout mice investigated lipid homeostasis. The results showed mouse...
In-depth coverage of proteomic analysis could enhance our understanding to the mechanism protein functions. Unfortunately, many highly hydrophobic proteins and low-abundance proteins, which play critical roles in signaling networks, are easily lost during sample preparation, mainly attributed fact that very few extractants can simultaneously satisfy requirements on strong solubilizing ability membrane good enzyme compatibility. Thus, it is urgent screen out ideal extractant from huge...
Protein N-phosphorylation, especially in mammals, was gradually studied and attracted widespread attention. However, the lack of effective enrichment methods resulted from intrinsic lability P-N bond under acidic conditions, which seriously hindered biological function elucidation. In this work, hydrophilic two-dimensional nanocomposites with enhanced hydrophilicity coordination interaction phosphate were designed as HILIC stationary phase, then HILIC-based strategy developed for...
Abstract Spatially resolved characterization of proteoforms has substantial potential to significantly advance the understanding physiological and disease mechanisms. However, challenges remain regarding throughput coverage. A robust method is developed for high‐throughput proteoform imaging (HTPi) by combining matrix‐assisted laser desorption ionization mass spectrometry (MALDI MSI) region‐specific top‐down proteomic analysis. MALDI MSI enables on tissue sections at a rate 7 h cm −2 (100‐µm...