A5049365368- Protein Structure and Dynamics
- RNA and protein synthesis mechanisms
- Enzyme Structure and Function
- Mass Spectrometry Techniques and Applications
- Polymer composites and self-healing
- RNA modifications and cancer
- Polymer Nanocomposites and Properties
- Ubiquitin and proteasome pathways
- Photosynthetic Processes and Mechanisms
- biodegradable polymer synthesis and properties
- Advanced Sensor and Energy Harvesting Materials
- Biotin and Related Studies
- Advanced NMR Techniques and Applications
- Advanced Proteomics Techniques and Applications
- RNA Research and Splicing
- Conducting polymers and applications
- Silicone and Siloxane Chemistry
- Click Chemistry and Applications
- Metabolomics and Mass Spectrometry Studies
- DNA and Nucleic Acid Chemistry
- Cancer-related gene regulation
- Neuropeptides and Animal Physiology
- Receptor Mechanisms and Signaling
- Analytical Chemistry and Chromatography
- Extracellular vesicles in disease
University of Chinese Academy of Sciences
2024-2025
Wuhan Institute of Physics and Mathematics
2016-2025
Wuhan National Laboratory for Optoelectronics
2023-2025
Chinese Academy of Sciences
2016-2025
Huazhong University of Science and Technology
2010-2025
South China University of Technology
2016-2024
Huazhong Agricultural University
2022
Photonics (United States)
2007
Insects detect and discriminate a diverse array of chemicals using odorant receptors (ORs), which are ligand-gated ion channels comprising divergent odorant-sensing OR conserved receptor co-receptor (Orco). In this work, we report structures the
Building tertiary structures of non-coding RNA is required to understand their functions and design new molecules. Current algorithms structure prediction give satisfactory accuracy only for small size simple topology many them need manual manipulation. Here, we present an automated fast program,3dRNA, with reasonable RNAs larger complex topology.
N 6 -methyladenosine (m A), a ubiquitous RNA modification, is installed by METTL3-METTL14 complex.The structure of the heterodimeric complex between methyltransferase domains (MTDs) METTL3 and METTL14 has been previously determined.However, MTDs alone possess no enzymatic activity.Here we present solution for zinc finger domain (ZFD) METTL3, inclusion which fulfills activity METTL3-METTL14.We show that ZFD specifically binds to an containing 5′-GGACU-3′ consensus sequence, but does not one...
Abstract UHRF1 is an important epigenetic regulator for maintenance DNA methylation. recognizes hemi-methylated (hm-DNA) and trimethylation of histone H3K9 (H3K9me3), but the regulatory mechanism remains unknown. Here we show that adopts a closed conformation, in which C-terminal region (Spacer) binds to tandem Tudor domain (TTD) inhibits H3K9me3 recognition, whereas SET-and-RING-associated (SRA) plant homeodomain (PHD) H3R2 recognition. Hm-DNA impairs intramolecular interactions promotes...
Abstract Autophagy receptor p62/SQSTM1 promotes the assembly and removal of ubiquitylated proteins by forming p62 bodies mediating their encapsulation in autophagosomes. Here we show that under nutrient-deficient conditions, cellular specifically undergoes acetylation, which is required for formation subsequent autophagic clearance bodies. We identify K420 K435 UBA domain as main acetylation sites, TIP60 HDAC6 acetyltransferase deacetylase. Mechanically, at both sites enhances binding to...
A robust, tough, and self-healable elastomer is a promising candidate for substrate in flexible electronic devices, but there often trade-off between mechanical properties (robustness toughness) self-healing. Here, poly(dimethylsiloxane) (PDMS) supramolecular developed based on metal-coordinated bonds with relatively high activation energy. The strong metal–coordination complexes their corresponding ionic clusters acting as the cross-linking points strengthen resultant networks, which...
Abstract Chemical cross-linking of proteins coupled with mass spectrometry is widely used in protein structural analysis. In this study we develop a class non-hydrolyzable amine-selective di- ortho -phthalaldehyde (DOPA) cross-linkers, one which called DOPA2. Cross-linking DOPA2 60–120 times faster than that the N-hydroxysuccinimide ester cross-linker DSS. Compared DSS cross-links, cross-links show better agreement crystal structures tested proteins. More importantly, has unique advantages...
Rabex-5 and Rabaptin-5 function together to activate Rab5 further promote early endosomal fusion in endocytosis. The GEF activity is autoinhibited by the CC domain (Rabex-5CC) activated C2-1 (Rabaptin-5C21) with yet unknown mechanism. We report here crystal structures of complex dimeric Rabaptin-5C21 (Rabaptin-5C212) Rabaptin-5C212 Rab5, along biophysical biochemical analyses. show that Rabex-5CC assumes an amphipathic α-helix which binds weakly substrate-binding site domain, leading weak...
The interaction between K48-linked ubiquitin (Ub) chain and Rpn13 is important for proteasomal degradation of ubiquitinated substrate proteins. Only the complex structure N-terminal domain (Rpn13NTD) Ub monomer has been characterized, while it remains unclear how specifically recognizes chain. Using single-molecule FRET, here we show that diubiquitin (K48-diUb) fluctuates among distinct conformational states, a preexisting compact state selectively enriched by Rpn13NTD. same binding mode...
Rubber is a strategically important polymeric material because of its high extensibility and resilience. However, traditional cross-linked rubber difficult to be reprocessed or recycled due the permanent covalent cross-linking, which puts huge burden on environment. Herein, we report covalently yet reprocessable carboxylated styrene butadiene (CSBR) nanocomposite reinforced by epoxy-modified tunicate cellulose nanocrystals (TCNCs). The epoxy modification TCNCs improves dispersibility...
Chemical crosslinking coupled with mass spectrometry (CXMS) has emerged as a powerful technique to obtain the dynamic conformations and interaction interfaces of protein complexes. Limited by poor cell membrane permeability, chemical reactivity, biocompatibility crosslinkers, in vivo capture dynamics complexes finer temporal resolution higher coverage is attractive but challenging. In this work, trifunctional crosslinker bis(succinimidyl) propargyl tag (BSP), involving compact size, proper...
Abstract Non-specific phospholipase C (NPC) hydrolyzes major membrane phospholipids to release diacylglycerol (DAG), a potent lipid-derived messenger regulating cell functions. Despite extensive studies on NPCs reveal their fundamental roles in plant growth and development, the mechanistic understanding of phospholipid-hydrolyzing by NPCs, remains largely unknown. Here we report crystal structure Arabidopsis NPC4 at resolution 2.1 Å. is divided into phosphoesterase domain (PD) C-terminal...
A polyubiquitin comprises multiple covalently linked ubiquitins and recognizes myriad targets. Free or bound to ligands, polyubiquitins are found in different arrangements of ubiquitin subunits. To understand the structural basis for quaternary plasticity explore target recognition mechanism, we characterize conformational space Lys63-linked diubiquitin (K63-Ub2). Refining against inter-subunit paramagnetic NMR data, show that free K63-Ub2 exists as a dynamic ensemble comprising closed open...
Chemical cross-linking coupled with mass spectroscopy (CXMS) provides proximity information for the cross-linked residues and is used increasingly modeling protein structures. However, experimentally identified cross-links are sometimes incompatible known structure of a protein, as distance calculated between far exceeds maximum length cross-linker. The discrepancies may persist even after eliminating potentially false excluding intermolecular ones. Thus "over-length" arise from alternative...
Significance Ubiquitination and phosphorylation are the two most important protein posttranslational modifications cell signals. Ubiquitin can be specifically phosphorylated at S65, finding here suggests a general functional role for Ub phosphorylation. We show that subtle fluctuation near physiological pH affect protonation status of S65 phosphoryl group modulate structure ubiquitin monomer polyubiquitin. It is known cellular varies among organelles changes under pathological conditions....