A5018789237- Hematopoietic Stem Cell Transplantation
- Mesenchymal stem cell research
- Transplantation: Methods and Outcomes
- Erythrocyte Function and Pathophysiology
- Immune Cell Function and Interaction
- Organ Transplantation Techniques and Outcomes
- Blood groups and transfusion
- RNA modifications and cancer
- Risk and Portfolio Optimization
- T-cell and B-cell Immunology
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Hemoglobinopathies and Related Disorders
- Cancer-related molecular mechanisms research
- Liver Disease and Transplantation
- Cancer-related gene regulation
- Blood disorders and treatments
- Cerebrovascular and Carotid Artery Diseases
- Complement system in diseases
- Fibroblast Growth Factor Research
- Chronic Lymphocytic Leukemia Research
- Immunotherapy and Immune Responses
- Neonatal Health and Biochemistry
- Acute Ischemic Stroke Management
- Platelet Disorders and Treatments
- Stochastic processes and financial applications
Affiliated Hospital of Nantong University
2020-2025
Nantong University
2020-2025
ETH Zurich
2023-2025
The First Affiliated Hospital, Sun Yat-sen University
2022-2025
Sun Yat-sen University
2020-2025
Yunnan Maternal and Child Health
2024-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2015-2025
Institute of Hematology & Blood Diseases Hospital
2016-2025
Key Laboratory of Guangdong Province
2023-2025
China International Science and Technology Cooperation
2023-2025
Wearable electronic devices are playing a rapidly expanding role in the acquisition of individuals' health data for personalized medical interventions; however, wearables cannot yet directly program gene-based therapies because lack direct electrogenetic interface. Here we provide missing link by developing an interface that call current (DC)-actuated regulation technology (DART), which enables electrode-mediated, time- and voltage-dependent transgene expression human cells using DC from...
N 6 -methyladenosine (m A), a ubiquitous RNA modification, is installed by METTL3-METTL14 complex.The structure of the heterodimeric complex between methyltransferase domains (MTDs) METTL3 and METTL14 has been previously determined.However, MTDs alone possess no enzymatic activity.Here we present solution for zinc finger domain (ZFD) METTL3, inclusion which fulfills activity METTL3-METTL14.We show that ZFD specifically binds to an containing 5′-GGACU-3′ consensus sequence, but does not one...
Abstract Background Longitudinal studies have verified the pivotal role of mesenchymal stem/stromal cells (MSCs) in bone marrow microenvironment for hematopoiesis and coordinate contribution to leukemia pathogenesis. However, precise characteristics alternation MSCs during acquired aplastic anemia (AA) remain obscure. Methods In this study, we originally collected samples from both healthy donors (HD) AA patients dissect hematological changes. To systematically evaluate biological defects...
Abstract Despite the array of mammalian transgene switches available for regulating therapeutic protein expression in response to small molecules or physical stimuli, issues remain, including cytotoxicity chemical inducers and limited biocompatibility cues. This study introduces gene driven by short peptides comprising eight fewer amino acid residues. Utilizing a competence regulator (ComR) sigma factor X‐inducing peptide (XIP) from Streptococcus vestibularis as receptor inducer,...
Idiopathic pulmonary hypertension (IPAH) is a condition that affects various tissues and organs the metabolic inflammatory systems. The most prevalent syndrome (MS), which involves insulin resistance, dyslipidemia, obesity. There may be connection between IPAH MS, based on plethora of studies, although underlying pathogenesis remains unclear. Through bioinformatics analyses machine learning algorithms, we identified 11 immune- metabolism-related potential diagnostic genes (EVI5L, RNASE2,...
Photosynthesis in plants occurs within specialized organelles known as chloroplasts, which are postulated to have originated through endosymbiosis with cyanobacteria. In nature, instances also observed wherein specific invertebrates engage symbiotic relationships photosynthetic bacteria, allowing them subsist photoautotrophic organisms over extended durations. Consequently, the concept of engineering artificial between mammalian cells and cyanobacteria represents a promising avenue for...
Abstract Synthetic biology aims to engineer transgene switches for precise therapeutic protein control in cell‐based gene therapies. However, off‐the‐shelf trigger‐inducible circuits are usually switched on by single or structurally similar molecules. This study presents a mammalian gene‐switch platform that controls expression wide range of molecules generating low, non‐toxic levels reactive oxygen species (ROS). In this system, KEAP1 (Kelch‐like ECH‐associated 1) serves as ROS sensor,...
Synthetic biology holds great promise to improve the safety and efficacy of future gene engineered cell therapies by providing new means endogenous or exogenous control embedded therapeutic programs. Here, we focused on gluconate as a clinically licensed small-molecule inducer gluconate-sensitive molecular switches regulate transgene expression in human cultures mice. Several switch designs were assembled based gluconate-responsive transcriptional repressor GntR from Escherichia coli....
Abstract Background The global incidence of liver diseases is rising, yet there remains a dearth precise research models to mimic these diseases. use normothermic machine perfusion (NMP) study diseased livers recovered from transplantation (LT) recipients presents promising avenue. Accordingly, we have developed system tailored specifically for the human whole and present our experience NMP livers. Methods Six LT with different diagnoses were collected. connected that circulated perfusate,...
Code comment plays an important role in program understanding, and a large number of automatic generation methods have been proposed recent years. To get better effect generating comments, many studies try to extract variety information (e.g., code tokens, AST traverse sequence, APIs call sequence) from source as model input. In this study, we found that the bytecode compiled can provide useful for generation, hence propose use assist generation. Specifically, control flow graph (CFG)...
Limited evidence exists on the effectiveness of endovascular treatment (EVT) for acute posterior circulation tandem lesion (PCTL). This study aimed to explore role extracranial vertebral artery (VA) stenting in patients with PCTL stroke undergoing EVT. Individual patient data were pooled from BASILAR (EVT Acute Basilar Artery Occlusion Study) and PERSIST (Posterior Circulation Ischemic Stroke) registries. Patients PCTLs who underwent EVT included present cohort divided into nonstenting...
Abstract Current small-molecule-regulated synthetic gene switches face clinical limitations such as cytotoxicity, long-term side-effects and metabolic disturbances. Here, we describe an a dvanced s ynthetic p latform i nducible by r isk-free in put medication (ASPIRIN), which is activated acetylsalicylic acid (ASA/aspirin), multifunctional drug with pain-relieving, anti-inflammatory, cardiovascular benefits. To construct ASPIRIN, repurpose plant salicylic receptors NPR1 NPR4. Through domain...
Concerns over health-related quality of life (HRQOL) in patients with aplastic anemia (AA) have been increasing worldwide. However, most researches on HRQOL AA ignored individual-level variability. Thus, our study was designed to explore practical classification and related variables among patients. A cross-sectional conducted from May 2022 March 2023, utilizing convenience sampling enroll Data HRQOL, sociodemographic characteristics, clinical were collected. Latent profile analysis (LPA)...