A5028134033- Virus-based gene therapy research
- Viral Infections and Immunology Research
- Autophagy in Disease and Therapy
- Amyotrophic Lateral Sclerosis Research
- RNA Interference and Gene Delivery
- RNA modifications and cancer
- CRISPR and Genetic Engineering
- interferon and immune responses
- Cytomegalovirus and herpesvirus research
- Prion Diseases and Protein Misfolding
- Cancer Genomics and Diagnostics
- Calcium signaling and nucleotide metabolism
- Neurogenetic and Muscular Disorders Research
- Cancer-related molecular mechanisms research
- Mosquito-borne diseases and control
- Genomics and Rare Diseases
- Renal and related cancers
- Diabetes and associated disorders
- RNA Research and Splicing
- Eosinophilic Disorders and Syndromes
- Genetic factors in colorectal cancer
- Plant-Microbe Interactions and Immunity
- IL-33, ST2, and ILC Pathways
- Immune Cell Function and Interaction
- Microbial metabolism and enzyme function
Jiangsu University
2025
Nanjing Medical University
2024
Changzhou Third People's Hospital
2024
St. Paul's Hospital
2017-2023
University of British Columbia
2017-2022
St. Paul's Hospital
2017-2022
Huazhong Agricultural University
2016-2021
Affiliated Hospital of Jining Medical University
2021
Tianjin Medical University General Hospital
2020
Insight Genetics (United States)
2020
Picornaviruses have evolved to hijack host cellular machinery, including the autophagic pathway. However, mechanisms remain largely unclear. We use coxsackievirus B3 (CVB3) as a model organism explore possible role of picornavirus subversion pathway in viral infection. Our vivo and vitro experiments demonstrate that CVB3 infection causes significant, albeit incomplete, inhibition flux by limiting fusion autophagosomes with lysosomes and/or late endosomes. Furthermore, we show specifically...
Genetic analyses of patients with amyotrophic lateral sclerosis (ALS) have revealed a strong association between mutations in genes encoding many RNA-binding proteins (RBPs), including TARDBP, FUS, hnRNPA1, hnRNPA2B1, MATR3, ATXN2, TAF15, TIA-1, and EWSR1, disease onset/progression. RBPs are group evolutionally conserved that participate multiple steps RNA metabolism, splicing, polyadenylation, mRNA stability, localization, translation. Dysregulation RBPs, as consequence gene mutations,...
Cell autonomous immunity is the ability of individual cells to initiate a first line host defense against invading microbes, such as viruses. Autophagy receptors, diverse family multivalent proteins, play key role in this response by detecting, sequestering, and eliminating virus process termed virophagy. To counteract this, positive-stranded RNA viruses, enteroviruses, have evolved strategies circumvent autophagic machinery an effort promote viral propagation; however, underlying mechanisms...
Summary Cellulose is one of the most abundant organic polymers in nature. It contains multiple β‐1,4‐glucan chains synthesized by cellulose synthases (CesAs) on plasma membrane higher plants. CesA subunits assemble into a pseudo‐sixfold symmetric synthase complex (CSC), known as ‘rosette complex’. The structure remains enigmatic. Here, we report cryo‐EM homotrimeric CesA7 from Gossypium hirsutum at 3.5‐angstrom resolution. GhCesA7 homotrimer shows C3 symmetrical assembly. Each protomer seven...
The ongoing pandemic of COVID-19 alongside the outbreaks SARS in 2003 and MERS 2012 underscore significance to understand betacoronaviruses as a global health challenge. SARS-CoV-2, etiological agent for COVID-19, has infected over 50 million individuals' worldwide with more than ∼1 fatalities. Autophagy modulators have emerged potential therapeutic candidates against SARS-CoV-2 but recent clinical setbacks urge better understanding viral subversion autophagy. Using MHV-A59 model...
Chronic kidney disease (CKD) is a major public health issue in the USA. Identification of monogenic causes CKD, which are present ∼10% adult cases, can impact prognosis and patient management. Broad gene panels provide unbiased testing approaches, advantageous phenotypically heterogeneous diseases. However, use yield broad genetic by nephrologists clinical practice not yet well characterized.Renal testing, ordered exclusively for purposes, predominantly general transplant within USA, was...
Neuroinflammation exacerbates the progression of SOD1-driven amyotrophic lateral sclerosis (ALS), although underlying mechanisms remain largely unknown. Herein, we demonstrate that misfolded SOD1 (SOD1Mut)-causing ALS results in mitochondrial damage, thus triggering release mtDNA and an RNA:DNA hybrid into cytosol mPTP-independent manner to activate IRF3- IFNAR-dependent type I interferon (IFN-I) interferon-stimulating genes. The neuronal hyper-IFN-I pro-inflammatory responses triggered...
Abstract Background Immunotherapy has emerged as an efficient therapeutic approach for cancer management. However, stimulation of host immune system against cells often fails to achieve promising clinical outcomes mainly owing the immunosuppressive characteristics tumor microenvironment (TME). Combination therapeutics that can trigger sustained immunogenic cell death (ICD) have provided new opportunities treatment. Methods In this study, we designed and applied ICD inducer regimen, including...
KRAS mutant (KRASmut ) lung adenocarcinoma is a refractory cancer without available targeted therapy. The current study explored the possibility to develop coxsackievirus type B3 (CVB3) as an oncolytic agent for treatment of KRASmut adenocarcinoma. In cultured cells, we discovered that CVB3 selectively infects and lyses cells (A549, H2030, H23), while sparing normal epithelial (primary, BEAS2B, HPL1D, 1HAEo) EGFRmut (HCC4006, PC9, H3255, H1975). Using stable expressing single driver mutation...
Coxsackievirus B3 (CVB3) is a prevalent etiological agent for viral myocarditis and neurological disorders, particularly in infants young children. Virus-encoded proteinases have emerged as cytopathic factors that contribute to disease pathogenesis part through targeting the cellular recycling machinery of autophagy. Although it appreciated CVB3 can usurp macroautophagy/autophagy pro-viral functions, precise mechanisms by which disrupt autophagy remain incompletely understood. Here we...
ABSTRACT The role for the NOD‐like receptor (NLR) P3 inflammasome in enterovirus infection remains controversial. Available data suggest that NLRP3 is protective against A71 but detrimental to host during coxsackievirus B3 (CVB3) infection. CVB3 a common etiologic agent associated with myocarditis and pancreatitis. Previous findings on of were based primarily indirect evidence. Here, we utilized knockout mice as well immune cardiac cells investigate direct interplay between activation. We...
Coxsackievirus B3 (CVB3) displays great oncolytic activity against various cancer cells. Previously, we demonstrated that adding targeting sequences (TS) of miR-145/143, which are downregulated in compared with normal cells, into CVB3 genome drastically attenuates tissue toxicity, while retaining its towards lung tumor. Here extended to assess miR-modified breast therapy. We generated a new miRNA-CVB3 by inserting TS muscle-specific miR-1 and pancreas-selective miR-216 the above...
A number of clinical trials demonstrated that tigecycline was effective and well tolerated in the treatment patients with various bacterial infections, but few literatures had shown coagulopathy induced by tigecycline. To address this concern, we performed a retrospective analysis to assess impact on coagulation parameters 50 infections our hospital (Shandong Provincial Hospital, China). These were treated at Shandong Hospital 2015-2016 either recommended (50 mg q12h) or higher dose (100...
We recently discovered that coxsackievirus B3 (CVB3) is a potent oncolytic virus against KRAS mutant lung adenocarcinoma. Nevertheless, the evident toxicity restricts use of wild-type (WT)-CVB3 for cancer therapy. The current study aims to engineer CVB3 decrease its and extend our previous research determine safety efficacy in treating TP53/RB1 small-cell (SCLC). A microRNA-modified (miR-CVB3) was generated via inserting multiple copies tumor-suppressive miR-145/miR-143 target sequences into...
Precision medicine aims to identify the right drug, for patient, at dose, time, which is particularly important in cancer therapy. Problems such as variability of treatment response and resistance medication have been long-standing challenges oncology, especially development new medications. Solid tumors, unlike hematologic malignancies or brain are remarkably diverse their cellular origins developmental timing. The ability next-generation sequencing (NGS) analyze comprehensive landscape...
Coxsackievirus B3 (CVB3) is a single-stranded positive RNA virus that usurps cellular machinery, including the evolutionarily anti-viral autophagy pathway, for productive infections. Despite emergence of double-membraned autophagosome-like vesicles during CVB3 infection, very little known about mechanism initiation. In this study, we investigated role established factors in initiation CVB3-induced autophagy. Using siRNA-mediated gene-silencing and CRISPR-Cas9-based gene-editing culture...