A5052061610- Microbial Natural Products and Biosynthesis
- Fungal Biology and Applications
- Marine Sponges and Natural Products
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Plant Pathogens and Fungal Diseases
- Viral Infectious Diseases and Gene Expression in Insects
- Tannin, Tannase and Anticancer Activities
- Metabolomics and Mass Spectrometry Studies
- Synthesis of Organic Compounds
- Invertebrate Immune Response Mechanisms
- Carbohydrate Chemistry and Synthesis
- CRISPR and Genetic Engineering
- Microbial Metabolism and Applications
- Advanced biosensing and bioanalysis techniques
- Crystal structures of chemical compounds
- Natural Compound Pharmacology Studies
- Peptidase Inhibition and Analysis
- Fermentation and Sensory Analysis
- Phytochemistry and Biological Activities
- Phytochemicals and Antioxidant Activities
- Essential Oils and Antimicrobial Activity
- Natural product bioactivities and synthesis
- Antimicrobial Peptides and Activities
- Synthetic Organic Chemistry Methods
State Key Laboratory of Respiratory Disease
2020-2025
Guangzhou Medical University
2013-2025
Ocean University of China
2023-2024
Chengdu University of Traditional Chinese Medicine
2024
Shanghai University of Traditional Chinese Medicine
2023
Zhongnan University of Economics and Law
2022
Scripps Institution of Oceanography
2017-2020
University of California, San Diego
2018
Guangxi Medical University
2016
Biogen (United States)
2012
Abstract Various algorithms comparing 2D NMR spectra have been explored for their ability to dereplicate natural products as well determine molecular structures. However, spectroscopic artefacts, solvent effects, and the interactive effect of functional group(s) on chemical shifts combine hinder effectiveness. Here, we leveraged Non-Uniform Sampling (NUS) techniques deep Convolutional Neural Networks (CNNs) create a tool, SMART, that can assist in discovery efforts. First, an NUS...
One known cyclic peptide, beauvericin, was isolated from the secondary metabolites of mangrove endophytic fungi Fusarium sp. (No. DZ27) in South China Sea. Its structure determined by spectral analyses and comparisons with reference data literatures. Beauvericin inhibited growth KB KBv200 cells potently IC50 values 5.76 ± 0.55 5.34 0.09 μM, respectively. Furthermore, beauvericin induced apoptosis through mitochondrial pathway, including decrease relative oxygen species generation, loss...
Because of the rapid increase in bacterial resistance, there is an urgent need for developing new antimicrobial agents to combat multidrug-resistant pathogens. In this study, we designed and synthesized a series kaempferol derivatives as biomimicking structural properties biological functions host defense peptides. After fine-tuning hydrophobic cationic hydrophilic moieties linked flavone scaffold kaempferol, obtained lead compound (52) that displayed high membrane selectivity (>128), poor...
The Citrus reticulata 'Chachi' pericarp (CRCP) is one cultivar of Citri Reticulatae Pericarpium (Chenpi), which widely applied in medicine and food. To determine the potential value CRCP harvested at different stages subjected to drying processes, dynamic changes bioactive components were profiled evaluated this study.The contents all non-volatile components, i.e. synephrine, limonin, phenolic acids flavonoids, decreased with delayed harvest time. volatiles thujene, α-pinene, β-pinene,...
A new monosesquiterpene diacetylgliocladic acid (1), a dimeric sesquiterpene divirensol H (9), and two exceptionally novel trimeric trivirensols B (11 12), together with another eight known congeners, were purified from an endophytic fungus Trichoderma virens FY06, derived Litchi chinensis Sonn. whose fruit is delicious popular food. All of them identified by comprehensive spectroscopic analysis, combined biosynthetic considerations. Trivirensols are unprecedented trimers which three...
Abstract To systematically identify the lipophilic constituents of Citri Reticulatae Pericarpium from different cultivars, supercritical CO 2 fluid extraction and ultra‐high‐performance liquid chromatography–Q Exactive Orbitrap tandem mass spectrometry were integrated for component analysis 18 batches 12 cultivars first time. A total 57 components extracts demonstrably or tentatively identified by obtained parent peaks, fragment retention times. In total, two flavonoids, six organic acids,...
White spot syndrome virus (WSSV) is one of the largest DNA viruses and major pathogen responsible for white in crustaceans. The WSSV capsid critical genome encapsulation ejection exhibits rod-shaped oval-shaped structures during viral life cycle. However, detailed architecture structural transition mechanism remain unclear. Here, using cryo-electron microscopy (cryo-EM), we obtained a cryo-EM model were able to characterize its ring-stacked assembly mechanism. Furthermore, identified an from...
Abstract Three new angucycline glycosides, designated grincamycin I (1), J (2), and K (3), together with the known congener A-7884 (4), were isolated from marine-derived actinomycete Streptomyces lusitanus SCSIO LR32. The structures of compounds elucidated by comprehensive spectral data analysis. Compounds 2 4 exhibited antitumor activity against human cancer cells MDA-MB-435, MDA-MB-231, NCI-H460, HCT-116 HepG2, normal breast epithelial cell MCF10A IC50 values ranging 0.4 to 6.9 µM. In...
Background: Apicidin, as an inhibitor of histone deacetylase, showed a wide range antiproliferative activity against various cancer cell lines. Apicidin has also been reported to induce apotosis via Fas/Fas ligand. Yet few studies have focused on mitochondrial pathway for its anti-tumor activity. Objective: In this study, we evaluated involved mechanism non-small lung GLC-82 cells. Method: was isolated from the mangrove endophtic fungi Fusarium sp. by solvent extraction and column...
Epigenetic regulation has emerged as a promising therapeutic strategy for lung cancer treatment, which can facilitate the antitumor responses by modulating epigenetic dysregulation of target proteins in cancer. The proteolysis-targeting chimera (PROTAC) reagent, dBET6 shows effective inhibition bromodomain-containing protein 4 (BRD4) that exerts efficacy degrading BRD4 via ubiquitin-proteasome system. Nevertheless, low tissue specificity and bioavailability impede its effects clinical...
Abstract A new natural product, named phomopsin A, 1‐(meta‐hydroxyphenyl)‐4‐hydroxy‐3‐isoquinolone (1), together with two known compounds cytochalasin H (2) and glucosylceramide (3), was isolated from the mangrove endophytic fungus Phomopsis sp. (ZZF08) obtained South China Sea coast. The structures were elucidated by 1D 2D NMR experiments including COSY, HMQC, HMBC. According to single‐crystal X‐ray diffraction, it found that some assignments about 1 13 C data for probably uncorrected in...
Abstract A copper coordination compound ZZF51(A) named bis(5‐butyl‐2‐pyridinecarboxylato‐N1,O2)‐copper, the first time found in nature, was isolated from a marine endophytic fungus Fusarium sp. ZZF51 South China Sea coast. Its structure elucidated using spectroscopic methods and single crystal X‐ray diffraction analysis. The antimicrobial cytotoxicity experiments exhibited that had mutagenicity activities against four aerobic reference strains Staphylococcus aureus , Bacillus subtilis...
Abstract In our ongoing investigation of the bioactive constituents from plants, two new lignans, magnolignan A‐2‐ O ‐β‐ D ‐glucopyranoside and strebluslignanol were isolated heartwood Streblus asper , along with three known A, magnolol, magnaldehyde D. 1D 2D NMR experiments, including COSY, HMQC, HMBC, other spectroscopic methods, UV, IR, MS used for determination structures assignments. Primary bioassays showed that have medium cytotoxic activity against HEp‐2 HepG2 cells, IC 50 13.3 µ M...
Cancer cell cytotoxicity was used to guide the isolation of nine new swinholide-related compounds, named samholides A–I (1–9), from an American Samoan marine cyanobacterium cf. Phormidium sp. Their structures were determined by extensive analysis 1D and 2D NMR spectroscopic data. The compounds share unusual 20-demethyl 44-membered lactone ring composed two monomers, they demonstrate structural diversity arising geometric isomerization double bonds, sugar units with unique glyceryl moieties...