A pair of unusual benzannulated 6,6-spiroketal enantiomers [(−)-1 and (+)-1] three new biogenetically related compounds (2–4), together with two known analogues (5 6), have been isolated from a mangrove fungus, Penicillium dipodomyicola HN4-3A. Their structures were elucidated on the basis spectroscopic analysis (1D 2D NMR data) X-ray crystallography. The absolute configurations (−)-1 (+)-1 determined using quantum chemical calculations electronic circular dichroic (ECD) spectra. Compounds 2...

Five α-pyrone meroterpenoids, including one new 3-epiarigsugacin E (1) and four known compounds, arisugacin D (2), B (3), territrem C (4) terreulactone (5) were obtained from the marine fungus Penicillium sp. SK5GW1L. Their structures identified by MS NMR experiments, absolute configuration of compound 1 was further confirmed low temperature (150 K) single crystal X-ray diffraction with Cu Kα radiation. Compounds 3, 4 5 showed strong inhibitory activities against acetylcholinesterase (AchE)...

Acid-induced arginine decarboxylase AdiA is a typical homo-oligomeric protein biosynthesizing alkaline nylon monomer putrescine. However, upon loss of the decamer oligomeric state at neutral and conditions activity also diminishes, obstructing whole-cell biosynthesis Here, structure cohesion strategy proposed to change pH adaptation environments based on rational engineering meridional latitudinal oligomerization interfaces. After integrating substitutions E467K interface H736E channel...

Three new phomoxanthone compounds, phomolactonexanthones A (1), B (2) and deacetylphomoxanthone C (3), along with five known phomoxanthones, including dicerandrol (4), (5), (6), (7) penexanthone (8), were isolated in the metabolites of fungus Phomopsis sp. HNY29-2B, which was from mangrove plants. The structures compounds 1–3 established on basis spectroscopic analysis. All evaluated against four human cancer cell lines breast MDA-MB-435, colon HCT-116, lung Calu-3 liver Huh7 by MTT assay....

Three new vermistatin derivatives, 6-demethylpenisimplicissin (<b>1</b>), 5′-hydroxypenisimplicissin (<b>2</b>), and 2′′-epihydroxydihydrovermistatin (<b>3</b>), along with five known analogues, methoxyvermistatin (<b>4</b>), (<b>5</b>), 6-demethylvermistatin (<b>6</b>), hydroxyvermistatin (<b>7</b>), penisimplicissin (<b>8</b>), were isolated from the culture of mangrove endophytic fungus <i>Penicillium</i> sp. HN29-3B1 <i>Cerbera manghas</i>. Their structures elucidated mainly by nuclear...

Abstract Background Icaritin is an aglycone of flavonoid glycosides from Herba Epimedii . It has good performance in the treatment hepatocellular carcinoma clinical trials. However, natural icaritin content very low. At present, mainly prepared by α-L-rhamnosidases and β-glucosidases two-step catalysis process. one-pot production required reported enzymes to be immobilized or bifunctional hydrolyze substrate with long reaction time, which caused complicated operations high costs. To improve...

Arigsugacin I (<b>1</b>), a new <i>α</i>-pyrone meroterpene, along with two known compounds, arigsugacins F (<b>2</b>) and territrem B (<b>3</b>), were isolated from the mangrove endophytic fungus <i>Penicillium</i> sp. sk5GW1L <i>Kandelia candel</i>. Their structures identified through mass spectrometry NMR experiments, absolute configuration of compound <b>1</b> was further confirmed by low-temperature (100 <i>K</i>) single crystal X-ray diffraction Cu K<i>α</i> radiation. The <b>2</b>...

The traditional Chinese medicine fufang preparation "Xian-Ling-Gu-Bao" capsule (XLGB), which is composed of six herbal medicines, popularly used for the treatment osteoporosis. A reliable and effective method using LC-linear ion trap (LTQ)/Orbitrap mass spectrometry rapid screening identification chemical constituents in described this paper. Based on UV spectrum, components isolated from original plants XLGB, 118 compounds were identified or tentatively characterized, including 58 flavonoid...

Abstract In this study, a rapid and sensitive method by ultra‐performance liquid chromatography coupled with quadrupole time‐of‐flight mass spectrometry, Metabolynx TM software defect filter technique was developed for screening identification of the metabolites in rat plasma after oral administration Shen‐Song‐Yang‐Xin capsule (SSYX). A total 92 SSYX‐related xenobiotics were identified or characterized, including 45 prototypes 47 metabolites. The results indicated that absorbed constituents...

Xylanthraquinone (1), a new anthraquinone, along with three known compounds, altersolanol A (2), deoxybostrycin (3) and bostrycin (4) was isolated from the fungus Xylaria sp. 2508 South China Sea. The structures of these compounds were identified by NMR experiments, absolute configuration compound 1 further confirmed single-crystal X-ray diffraction Cu Kα radiation. Compounds 1–4 did not show inhibitory activities against Mycobacterium tuberculosis protein tyrosine phosphatase B (IC50 values...

Hemiberlesia pitysophila Takagi is an extremely harmful exotic insect in forest to Pinus species, including massoniana. Using both morphological taxonomy and molecular phylogenetics, we identified 15 strains of entomogenous fungi, which belong 9 genera with high diversities. Surprisingly, found that five were classified as species Pestalotiopsis, has been considered plant pathogens endophytes, the dominant entomopathogenic fungus H. pitysophila. Molecular phylogenetic tree established by...

Abstract Three new chromone derivatives, phomochromenones A‐C ( 1–3 ), and one known chaetocyclinone B 4 ) were obtained from the cultures of Phomopsis sp. HNY29‐2B isolated mangrove Acanthus ilicifolius Linn., which was collected South China Sea. Their structures determined by analysis 1 D NMR 2 as well mass spectroscopic data. The absolute configurations assigned quantum chemical calculations electronic circular dichroism ECD spectra. Compound 3 is third example alkaloids possessing unique...

Four spiroalkaloids, including a new compound shensongine A (1), were isolated from the anti-arrhythmic TCM formula Shensong Yangxin capsule. Their structures determined on basis of spectroscopic analysis. Compounds 1 and 3 displayed cardiovascular activities by shortened APD in rat myocardial cells. These compounds possibly generated precursors different composed herbal medicines during processing formula.

Abstract Two new iridoids, 10‐ O ‐benzoyl‐6′‐ ‐ α L ‐arabino(1→6)‐ β D ‐glucopyranosylgeniposidic acid ( 1 ), deacetyl‐6‐ethoxyasperulosidic methyl ester 2 along with 14 other known were isolated from the whole plant of Hedyotis diffusa Willd. Their structures determined on basis spectroscopic analysis. The short‐term‐memory‐enhancement activities compounds 1, 7 – 9, 11 , and 13 evaluated an A transgenic drosophila model.

Chinese tonic liquor is an important dietary supplement in daily use, but it often happens that illicitly adulterated drugs could lead to food safety issues. In this survey, analytical method consisting of a liquid chromatography-quadrupole-time flight mass spectrometer (LC-Q-TOF-MS), coupled with quick easy cheap effective rugged safe (QuEChERS) pretreatment, was established for identification phosphodiesterase type 5 enzyme (PDE-5) inhibitors liquors. 86 PDE-5 were qualitatively analysed...