The endosomal sorting complex required for transport (ESCRT) complexes function to form multivesicular bodies of proteins destined the yeast vacuole or mammalian lysosome. ESCRT components are well conserved in eukaryotes, and their mutations cause neurodegenerative diseases other cellular pathologies humans. PMR1 is orthologous gene two human genes calcium pumps secretory pathway Ca2+-ATPase (SPCA1, ATP2C1) sarco/endoplasmic reticulum (SERCA, ATP2A2), which mutated Hailey–Hailey Darier...

Abstract Background Sodium dodecyl sulfate (SDS) is one of the most widely used anionic alkyl surfactants. Toxicological information on SDS accumulating, however, mechanisms toxicity regulation remain poorly understood. In this study, relationship between SDS-sensitive mutants and their intracellular ROS levels has been investigated. Results Through a genome-scale screen, we have identified 108 yeast single-gene deletion that are sensitive to 0.03% SDS. These genes were predominantly related...

Pulsed electromagnetic field (PEMF) therapy has been extensively investigated in clinical studies for the treatment of angiogenesis-related diseases. However, there is a lack research on impact PEMFs energy metabolism and mitochondrial dynamics during angiogenesis. The present study included tube formation CCK-8 assays. A Seahorse assay was conducted to analyze metabolism, membrane potential assays, imaging, reactive oxygen species assays were used measure changes structure function human...

Zinc is essential for almost all living organisms, since it serves as a crucial cofactor transcription factors and enzymes. However, toxic to cell growth when present in excess. The work aims investigate the toxicity mechanisms induced by zinc stress yeast cells. To this end, 108 single-gene deletion mutants were identified sensitive 6 mM ZnCl2 through genome-wide screen. These genes predominantly related biological processes of vacuolar acidification transport, polyphosphate metabolic...

Malonic acid (MA) is a high-value chemical with diverse applications in the fields of food, agriculture, medicine, and synthesis. Despite successful biosynthesis MA has been performed Escherichia coli, Myceliophthora thermophila, Saccharomyces cerevisiae, resulting titers remain insufficient for industrial-scale production. In this study, three distinct metabolic pathways were designed constructed to increase production E. coli. Among these, fumaric pathway comprising four key enzymes...

Cadmium is a carcinogen that can induce ER stress, DNA damage, oxidative stress and cell death. The yeast mitogen-activated protein kinase (MAPK) signalling pathways paly crucial roles in response to various stresses. Here, we demonstrate the unfolded (UPR) pathway, high osmolarity glycerol (HOG) pathway wall integrity (CWI) are all essential for cells defend against cadmium-induced toxicity, including elevated ROS death levels induced by cadmium. We show UPR required phosphorylation of...

Type 2C protein phosphatases (PP2C) are monomeric enzymes that require magnesium or manganese ions for their activities. There seven PP2C genes in Candida albicans. Here, we demonstrate CaPTC5 encodes a mitochondrial enzyme. Expression of transcripts remains constant during the serum-induced hyphal development. Deletion does not affect vitro filamentation but renders C. albicans cells sensitive to terbinafine and cadmium, this sensitivity is complemented by Saccharomyces cerevisiae ScPTC5....

Chemical energy stored in sludge can be directly converted into electricity using sediment microbial fuel cell (SMFC) technology.

ESCRT components function to form multivesicular bodies for sorting of proteins destined the yeast vacuole. The calcium hypersensitivity mutants is mainly due repressed expression PMR1 through Rim101/Nrg1 pathway in budding yeast. Here, we show that overexpression PMC1 and its negative regulator gene NYV1 suppresses increases mutants, respectively. Consistently, deletion their hypersensitivity. Expression dramatically reduced mutants. Promoter analysis demonstrates both Nrg1 Mig1 repress...

The calcium/calcineurin signalling pathway is required for cell survival under various environmental stresses. Using Saccharomyces cerevisiae, we explored the mechanism underlying calcium-regulated homeostasis of intracellular reactive oxygen species (ROS). We found that deletion acyltransferase Akr1 and C-5 sterol desaturase Erg3 increased ROS levels death, this could be inhibited by addition calcium. hexose transporter Hxt1 amino acid permease Agp1 play crucial roles in maintaining levels,...

The Vcx1-M1 mutant is known to confer calcineurin-dependent Mn(2+) tolerance in budding yeast. Here, we demonstrate that another Vcx1 mutant, Vcx1-D1 with calcineurin-independent vacuolar Ca(2+)/H(+) exchanger activity, confers tolerance. Unlike Vcx1-M1, the conferred by dependent on presence of Pmr1 or Pmc1. Pmr1-dependent requires calcineurin but not functioning Ca(2+)/calcineurin signaling pathway. Similar wild-type Vcx1, C-terminally green fluorescent protein tagged and mutants localize...

ABSTRACT As a potentially toxic heavy metal, Cadmium (Cd) can cause endoplasmic reticulum and oxidative stress, thus lead to cell death. To explore the mechanisms of Cd toxicity, we investigated UPRE-lacZ expression, intracellular reactive oxygen species (ROS) death in 151 Cd-sensitive mutants Saccharomyces cerevisiae response stress. We identified 101 genes regulating expression were involved preventing ROS production and/or from increasing high levels, while for 72 caused both elevated...

Abstract Background: Sodium dodecyl sulfate (SDS) is one of the most widely used anionic alkyl surfactants. Toxicological information on SDS accumulating, however, mechanisms toxicity regulation remain poorly understood. In this study, relationship between SDS-sensitive mutants and their intracellular ROS levels has been investigated. Results: Through a genome-scale screen, we have identified 108 yeast single-gene deletion that are sensitive to 0.03% SDS. These genes were predominantly...

Abstract Background: Sodium dodecyl sulfate (SDS) is one of the most widely used anionic alkyl surfactants. Toxicological information on SDS accumulating, however, mechanisms toxicity regulation remain poorly understood. In this study, relationship between SDS-sensitive mutants and their intracellular ROS levels has been investigated. Results: Through a genome-scale screen, we have identified 108 yeast single-gene deletion that are sensitive to 0.03% SDS. These genes were predominantly...

Abstract Background: Sodium dodecyl sulfate (SDS) is one of the most widely used anionic alkyl surfactants. Toxicological information on SDS accumulating, however, mechanisms toxicity regulation remain poorly understood. In this study, relationship between SDS-sensitive mutants and their intracellular ROS levels has been investigated. Results : Through a genome-scale screen, we have identified 108 yeast single-gene deletion that are sensitive to 0.03% SDS. These genes were predominantly...