A5080577317- Pluripotent Stem Cells Research
- Cancer-related gene regulation
- Animal Genetics and Reproduction
- Plant Virus Research Studies
- Neurogenesis and neuroplasticity mechanisms
- Reproductive Biology and Fertility
- Epigenetics and DNA Methylation
- Wnt/β-catenin signaling in development and cancer
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Signaling Pathways in Disease
- Agricultural Science and Fertilization
- Sperm and Testicular Function
- NF-κB Signaling Pathways
- Insect-Plant Interactions and Control
- Growth Hormone and Insulin-like Growth Factors
- Lipoproteins and Cardiovascular Health
- Food Industry and Aquatic Biology
- Pancreatic and Hepatic Oncology Research
- Microbial metabolism and enzyme function
- RNA and protein synthesis mechanisms
- Ubiquitin and proteasome pathways
- Nerve injury and regeneration
- Heavy metals in environment
- Reproductive Physiology in Livestock
- Transgenic Plants and Applications
Zhangqiu City People's Hospital
2024
Tobacco Research Institute
2024
Beijing Institute of Genomics
2024
Chinese Academy of Sciences
2012-2024
Hunan University
2023
Shanghai Institutes for Biological Sciences
2012-2020
Center for Excellence in Molecular Plant Sciences
2020
Marine Biodiversity Exploitation and Conservation
2019
Kunming Institute of Botany
2018
Northwest A&F University
2011-2015
Dishevelled (Dvl) is a key component in the canonical Wnt signaling pathway and becomes hyperphosphorylated upon stimulation. Dvl required for LRP6 phosphorylation, which essential subsequent steps of signal transduction, such as Axin recruitment cytosolic β-catenin stabilization. Here, we identify HECT-containing Nedd4-like ubiquitin E3 ligase ITCH new Dvl-binding protein. ubiquitinates phosphorylated form promotes its degradation via proteasome pathway, thereby inhibiting signaling....
Simple sequence repeats (SSRs) are tandem of DNA that have been used to develop robust genetic markers. These molecular markers powerful tools for basic and applied studies such as breeding. In the model plants in Nicotiana genus e.g. N. benthamiana, a comprehensive assessment SSR content has become possible now because several genomes sequenced. We conducted genome-wide characterization marker development across seven genomes.Here, we initially characterized 2,483,032 SSRs (repeat units...
The Ca2+ signaling pathway appears to regulate the processes of early development through its antagonism canonical Wnt/β-catenin pathway. However, underlying mechanism is still poorly understood. Here, we show that nuclear factor activated T cells (NFAT), a component signaling, interacts directly with Dishevelled (Dvl) in Ca2+-dependent manner. A dominant negative form NFAT rescued inhibition triggered by signal. functioned downstream β-catenin without interfering stability, but influencing...
Previous studies have shown that promyelocytic leukaemia zinc finger (PLZF) is a spermatogonia-specific transcription factor in the testis, required to regulate self-renewal and maintenance of spermatogonia stem cell. Up now, expression function PLZF goat testis has not been known. The objectives this study were investigate pattern dairy its effect on male germline cell (mGSC) differentiation.Testis development patterns analysed by haematoxylin eosin staining, immunohistochemistry reverse...
Ribonucleic acid sequencing (RNA-Seq) libraries are normally prepared with oligo(dT) selection of poly(A)+ mRNA, but it depends on intact total RNA samples. Recent studies have described Ribo-Zero technology, a novel method that can capture both and poly(A)− transcripts from or fragmented We report here the first application RNA-Seq for analysis bovine embryonic, neonatal, adult skeletal muscle whole transcriptome at an unprecedented depth. Overall, 19,893 genes were found to be expressed,...
Phytoene synthase (PSY) has been considered as an important regulatory enzyme in carotenoids biosynthesis pathway. Previous study finds that the yellow fruit Solanum lycopersicum var. cerasiforme accession PI 114490 is caused by loss-of-function of SlPSY1 due to trans-splicing between SlPsy1 and unknown gene transcribed from neighbour opposite strand DNA SlPsy1. The genomic sequences red yellow-fruited tomato lines have one single-nucleotide polymorphism (SNP) fourth intron SSR intergenic...
Abstract Objectives Nanos2, an RNA ‐binding protein, belongs to the Nanos gene‐coding family and contains two CCHC zinc‐finger motifs. In mouse, it plays a pivotal role in male germ cell development, self‐renewal of spermatogonial stem cells. However, little is known its expression pattern functions dairy goat testis. Materials methods Immunohistochemistry quantitative reverse transcription‐polymerase chain reaction ( qRT ‐ PCR ) were used generate profile Nanos2 Furthermore, overexpression...
Abstract Neural progenitor cells (NPCs) are essential for in vitro drug screening and cell-based therapies brain-related disorders, necessitating well-defined reproducible culture systems. Current strategies employing protein growth factors pose challenges terms of both reproducibility cost. In this study, we developed a novel DNA-based modulator to regulate FGFR signaling NPCs, thereby facilitating the long-term maintenance stemness promoting neurogenesis. This FGFR-agonist effectively...
Abstract The nicotinic acetylcholine receptors (nAChR) are complexes that composed of subunits evolved from common ancestor. Although the similar in sequences and structures, their molecular function varies significantly. Therefore, detecting sites specific to each subunit is important understand property formed by them. As we know, critical structure a protein family usually conserved evolution, those member often closely related its structural functional specificity. In this study,...
Abstract Circulating cell-free DNA (cfDNA) in the peripheral blood is a promising biomarker for cancer diagnosis and prognosis. Somatic mutations identified cancers have been used to detect therapeutic targets clinical transformation individualize drug selection, while germline variants can predict patient’s risk of developing sensitivity. However, no platform has developed analyze, calculate, integrate, friendly visualize these pan-cancer cfDNA deeply. In this work, we performed panel...
Abdominal aortic aneurysm (AAA) is a complex and fatal vascular disease for which specific treatments are still lacking. This study explored the effect possible mechanisms of prenyl diphosphate synthase subunit 2 (PDSS2) on angiotensin II (Ang II)-induced AAA in human smooth muscle cells (VSMCs). The cell model was established by treating VSMCs with 1 μM Ang 24 h. VSMC viability detected counting kit-8 assay. role PDSS2 proliferation examined using 5-ethynyl-2'-deoxyuridine method. influence...
Introns have been shown to be spliced in a defined order, and this order influences both alternative splicing regulation fidelity, but previous studies only considered neighbouring introns. The detailed intron remains unknown.In work, method was developed that can calculate the orders of all introns each transcript. A simulation study showed accurately orders. I further applied real S. pombe, fruit fly, Arabidopsis thaliana, human sequencing datasets found change from gene humans contain...
Abstract Neural progenitor cells (NPCs) are important for in vitro drug screening and the cell-based therapy brain-related disorders, which requires well-defined reproducible culture systems. Current strategy use of protein growth factors presents challenges terms reproducibility cost. In this study, we have developed a novel DNA-based modulator to regulate FGFR signaling NPCs, enabling maintenance stemness over 50 passages neurogenesis towards neurons. The FGFR-agonist effectively promoted...
Abstract. The transcription factor nuclear factor1-C2 (NF1-C2) mediates the action of prolactin in mammary gland. Research on molecular genetic mechanism model system have indicated that NF1-C2 gene plays an important role for activation several gland specific genes (Nilsson et al. 2006). Moreover, effects milk production traits been reported ruminant (Seriwatanachai 2008). may a similar function goat. To our knowledge polymorphisms not described animals. Here SNP caprine was detected and...
Most older methods of fish farming, or aquaculture, have focused on growing only a single species sea life, for example, salmon. Modern aquaculture systems involve the cultivation two more together, based what happens normally in food chain, so that one can provide source another farm. This article describes results an experiment combining culture with algae and oyster culture. We show grow using waste, oysters eat to produce good-quality, healthy food, which reduces pollution generated by...