A5043915667- Microbial Community Ecology and Physiology
- Gut microbiota and health
- Genomics and Phylogenetic Studies
- Ocular Surface and Contact Lens
- Wastewater Treatment and Nitrogen Removal
- Ocular Infections and Treatments
- Ferroptosis and cancer prognosis
- COVID-19 Clinical Research Studies
- Cancer Immunotherapy and Biomarkers
- SARS-CoV-2 and COVID-19 Research
- Single-cell and spatial transcriptomics
- Epigenetics and DNA Methylation
- Water Treatment and Disinfection
- Air Quality Monitoring and Forecasting
- Air Quality and Health Impacts
- Microbial Fuel Cells and Bioremediation
- Methane Hydrates and Related Phenomena
- Extracellular vesicles in disease
- Oral microbiology and periodontitis research
- Glaucoma and retinal disorders
- DNA and Nucleic Acid Chemistry
- RNA and protein synthesis mechanisms
- Retinal Diseases and Treatments
- Liver Disease and Transplantation
- Immune Cell Function and Interaction
Central South University
2022-2025
Sun Yat-sen University
2018-2024
Second Affiliated Hospital of Nanchang University
2024
Nanchang University
2020-2024
WuXi AppTec (China)
2024
Taihe Hospital
2024
Fudan University
2016-2023
Ningxia Medical University
2023
Janssen (United States)
2023
Changsha Central Hospital
2022
The discovery of complete ammonia oxidizers (comammox) refutes the century-old paradigm that nitrification requires activity two types microbes. Determining distribution and abundance comammox in various environments is important for revealing ecology microbial within global nitrogen cycle. In this study, ubiquity diversity were analyzed samples from different environments, including soil, sediment, sludge, water. results a two-step PCR using highly degenerate primers (THDP-PCR) quantitative...
Abstract Background Acute glaucoma, characterized by a sudden elevation in intraocular pressure (IOP) and retinal ganglion cells (RGCs) death, is major cause of irreversible blindness worldwide that lacks approved effective therapies, validated treatment targets clear molecular mechanisms. We sought to explore the potential mechanisms underlying causal link between high IOP glaucomatous RGCs death. Methods A murine ischemia/ reperfusion (RIR) model an vitro oxygen glucose...
Ameliorating T cell exhaustion and enhancing effector function are promising strategies for the improvement of immunotherapies. Here, we show that HPK1-NFκB-Blimp1 axis mediates dysfunction. High expression MAP4K1 (which encodes HPK1) correlates with increased worse patient survival in several cancer types. In MAP4K1KO mice, tumors grow slower than wild-type mice infiltrating cells less exhausted more active proliferative. We further genetic depletion, pharmacological inhibition, or...
Abstract Large-scale metagenomic assemblies have uncovered thousands of new species greatly expanding the known diversity microbiomes in specific habitats. To investigate roles these uncultured human health or environment, researchers need to incorporate their genome into a reference database for taxonomic classification. However, this procedure is hindered by lack well-curated tree newly discovered species, which required current metagenomics tools. Here we report DeepMicrobes, deep...
Nitrospira is a dominant nitrite-oxidizing bacterium in many artificial and natural environments. The discovery of complete ammonia oxidizers the genus prevents use previously identified primers targeting 16S rRNA gene or nitrite oxidoreductase ( nxr ) for differential determination strict bacteria (sNOB) among comammox this genus. We designed three novel primer sets that enabled quantification clades A B sNOB with high coverage, specificity, accuracy various With sets, were differentially...
Abstract The current dogma in ophthalmology and vision research presumes the intraocular environment to be sterile. However, recent evidence of intestinal bacterial translocation into bloodstream many other internal organs including eyes, found healthy diseased animal models, suggests that cavity may also inhabited by a microbial community. Here, we tested samples from over 1000 human eyes. Using quantitative PCR, negative staining transmission electron microscopy, direct culture,...
The bromodomain and extra-terminal domain (BET) proteins are promising therapeutic targets to treat refractory solid tumors; however, inherent resistance remains a major challenge in the clinic. Recently, emerging role of oncoprotein B cell lymphoma 6 (BCL6) tumorigenesis stress response has been unveiled. Here, we demonstrate that BCL6 was upregulated upon BET inhibition KRAS-mutant cancers, including non–small-cell lung cancer (NSCLC). We further found BRD3, not BRD2 or BRD4, directly...
Purpose: Microbial ecosystems interact with the human body and affect health. The microbial community on ocular surface remains an underexplored territory despite its importance as first line of defense barrier that protects eye ultimately sight. We investigated how age sex affected microbiome, in present study wanted to understand geographic difference shaped microbiome surface. Methods: collected conjunctival specimens 172 eyes from 86 healthy volunteers living three Chinese cities,...
The new coronavirus Covid-19 has spread globally and caused a pneumonia outbreak. Although the whole genome sequences of have been rapidly obtained, pathogenesis severe disease remains elusive. Understanding immune responses in patients with may significantly advance development effective therapies. Here, using scRNA-seq technology, we profiled immunophenotypes four patients' PBMCs before, during, after their ICU cares. Our data identified gene expression signatures viral invasion type I...
Abstract Ameloblastoma is a benign tumor characterized by locally invasive phenotypes, leading to facial bone destruction and high recurrence rate. However, the mechanisms governing initiation are poorly understood. Here, we uncovered cellular landscapes that underlie in ameloblastoma at single-cell resolution. Our results revealed exhibits five subpopulations varying with respect immune response (IR), remodeling (BR), tooth development (TD), epithelial (ED), cell cycle (CC) signatures. Of...
The copper-containing membrane-bound monooxygenase (CuMMO) family comprises key enzymes for methane or ammonia oxidation: particulate (PMMO) and (AMO). To comprehensively amplify CuMMO genes, a two-step PCR strategy was developed using newly designed tagged highly degenerate primer (THDP; degeneracy = 4608). Designated THDP-PCR, the technique consists of primary gene-specific followed by secondary with tag as single primer. No significant bias in THDP-PCR amplification found various...
The bone marrow niche plays a critical role in controlling the fate of hematopoietic stem cells (HSCs) by integrating intrinsic and extrinsic signals. However, molecular events HSC remain to be investigated. Here, we report that intercellular adhesion molecule-1 (ICAM-1) maintains quiescence repopulation capacity niche. ICAM-1-deficient mice (ICAM-1−/−) displayed significant expansion phenotypic long-term HSCs with impaired quiescence, as well favoring myeloid cell expansion. presented...
Significance The mechanisms underlying gut follicular helper T (T FH ) cells and their role in mucosal homeostasis is not fully understood. In this study, we demonstrate that activating transcription factor 3 (ATF3) represents a regulator of TFH cells, which dictates the susceptibility colitis. Bcl6 was identified as transcriptional target ATF3 cells.
It is well recognized that batch effect in single-cell RNA sequencing (scRNA-seq) data remains a big challenge when integrating different datasets. Here, we proposed deepMNN, novel deep learning-based method to correct scRNA-seq data. We first searched mutual nearest neighbor (MNN) pairs across batches principal component analysis (PCA) subspace. Subsequently, correction network was constructed by stacking two residual blocks and further applied for the removal of effects. The loss function...
Combined hepatitis A and B vaccine administered on an accelerated schedule provides a rapid immune response against both viruses, which might be especially relevant for individuals who need protection quickly.A prospective, open-label, randomized study to compare the immunogenicity reactogenicity of combined Twinrix (GlaxoSmithKline Biologicals, Rixensart, Belgium) (>or=720 EL.U/mL inactivated antigen 20 microg/mL recombinant surface [HBsAg]) at 0, 7, 21 30 days, 12 months compared with...
The new coronavirus COVID-19 has spread globally and caused a pneumonia outbreak. Although the whole genome sequences of have been rapidly obtained, pathogenesis severe disease remains elusive. Understanding immune responses in patients with may significantly advance development effective therapies. Here, using single cell RNA-seq technology, we profiled immunophenotypes four patients' PBMCs before, during, after their ICU cares. Our data identified gene expression signatures viral invasion...
Photoreceptor connecting cilium (CC) is structurally analogous to the transition zone (TZ) of primary cilia and gates molecular trafficking between inner outer segment (OS). Retinal dystrophies with underlying CC defects are manifested in a broad array syndromic conditions known as ciliopathies well nonsyndromic retinal degenerations. Despite extensive studies, many questions remain mechanism protein across photoreceptor CC. Here, we genetically inactivated mouse Tmem138, gene encoding...