A5100769590- Liver Disease Diagnosis and Treatment
- Cancer, Hypoxia, and Metabolism
- Autophagy in Disease and Therapy
- Liver Disease and Transplantation
- Cell death mechanisms and regulation
- Metabolism, Diabetes, and Cancer
- Fungal Biology and Applications
- Liver physiology and pathology
- Peroxisome Proliferator-Activated Receptors
- Cancer-related molecular mechanisms research
- Liver Diseases and Immunity
- Cancer Cells and Metastasis
- Epigenetics and DNA Methylation
- Histone Deacetylase Inhibitors Research
- MicroRNA in disease regulation
- Hepatocellular Carcinoma Treatment and Prognosis
- Wnt/β-catenin signaling in development and cancer
- Pancreatic function and diabetes
- Retinoids in leukemia and cellular processes
- Cancer Research and Treatments
- RNA modifications and cancer
- Cancer therapeutics and mechanisms
- Organic Electronics and Photovoltaics
- Lysosomal Storage Disorders Research
- Heme Oxygenase-1 and Carbon Monoxide
Shanghai Jiao Tong University
2025
Shanghai First People's Hospital
2025
Zhejiang University of Technology
2024
Zhejiang University
2005-2024
Sir Run Run Shaw Hospital
2019-2024
Yangtze University
2023-2024
Soochow University
2021-2024
Air Force Medical University
2018-2024
Sun Yat-sen University
2022-2024
Capital Medical University
2017-2024
Abstract Tumor cells generally proliferate rapidly and the demand for essential nutrients as well oxygen always exceeds supply due to unregulated growth insufficient inappropriate vascular supply. However, cancer show an inherent ability tolerate extreme conditions, such that characterized by low nutrient supply, modulating their energy metabolism. Thus, targeting nutrient-deprived may be a novel strategy in anticancer drug development. Based on that, we established screening method discover...
Abstract The aim of this study was to investigate the effect quercetin on hepatic fibrosis, a characteristic response acute or chronic liver injury. Mice were randomized bile duct ligation (BDL) carbon tetrachloride (CCl 4 ) cirrhosis models. Quercetin (100 mg/kg 200 daily) administered by gavage for 2 weeks. Liver tissue and blood samples collected histological molecular analysis. results our experiments showed that reduced BDL CCl inhibited extracellular matrix formation, regulated...
An extremely halotolerant and alkaliphilic bacterium was isolated previously from deep-sea sediment collected at a depth of 1050 m on the Iheya Ridge. The strain, designated HTE831 (JCM 11309, DSM 14371), Gram-positive, strictly aerobic, rod-shaped, motile by peritrichous flagella, spore-forming. Strain grew salinities 0–21% (w/v) NaCl pH 7.5 0–18% 9.5. optimum concentration for growth 3% both G+C content its DNA 35.8%. Low level (12–30%) DNA–DNA relatedness between strain species these...
Coronary arteries bring blood flow to the heart muscle. Understanding developmental program of coronary provides insights into treatment artery diseases. Multiple sources have been described as contributing including proepicardium, sinus venosus (SV), and endocardium. However, origins vessels are still under intense study. We produced a new genetic tool for studying development, an AplnCreER mouse line, which expresses inducible Cre recombinase specifically in developing vessels....
A variety of mechanisms maintain the integrity genome in face cell stress. Cancer response to chemotherapeutic and radiation-induced DNA damage is mediated by multiple defense including polo-like kinase 1 (Plk-1), protein B (Akt-1), and/or p53 pathways leading either apoptosis or cycle arrest. Subsequently, a subpopulation arrested viable cancer cells may remain recur despite aggressive repetitive therapy. Here, we show that modulation (activation Akt-1 Plk-1 repression p53) these...
Background: Hepatic ischemia reperfusion (IR) is an important issue in complex liver resection and transplantation. The aim of the present study was to determine protective effect astaxanthin (ASX), antioxidant, on hepatic IR injury via reactive oxygen species/mitogen-activated protein kinase (ROS/MAPK) pathway. Methods: Mice were randomized into a sham, IR, ASX or + group. mice received at different doses (30 mg/kg 60 mg/kg) for 14 days. Serum tissue samples 2 h, 8 h 24 after abdominal...
Genistein is a natural isoflavone with many health benefits, including antitumour effects. Increased hypoxia-inducible factor 1 α (HIF-1α) levels and glycolysis in tumour cells are associated an increased risk of mortality, cancer progression, resistance to therapy. However, the effect genistein on HIF-1α hepatocellular carcinoma (HCC) still unclear. Cell viability, apoptosis rate, lactate production, glucose uptake were measured HCC cell lines incubation. Lentivirus-expressed transporter...
Hepatocytes are functionally heterogeneous and divided into two distinct populations based on their metabolic zonation: the periportal pericentral hepatocytes. During liver injury regeneration, cellular dynamics of these remain largely elusive. Here we show that major facilitator super family domain containing 2a (Mfsd2a), previously known to maintain blood-brain barrier function, is a zonation marker. By genetic lineage tracing Mfsd2a+ hepatocytes, population decreases during homeostasis....
// Weiqi Dai 1,* , Fan Wang Jie Lu Yujing Xia 1 Lei He Kan Chen Jingjing Li Sainan Tong Liu Yuanyuan Zheng Jianrong 1,2 Wenxia Yuqing Zhou 1,3 Qin Yin Huerxidan Abudumijiti Rongxia Rong Zhang Zhu Jing Yang Chengfen Huawei Yingqun Ling Xu 4 and Chuanyong Guo Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University School Medicine, Shanghai, China 2 The First Clinical Medical College Nanjing University, Nanjing, 3 Affiliated Hospital Soochow Suzhou, Tongren Jiaotong...
The anti-tumor antibiotic salinomycin (Sal) was recently identified as a selective inhibitor of breast cancer stem cells; however, the effect Sal on hepatocellular carcinoma (HCC) is not clear. This study aimed to determine efficacy and mechanism HCC. HCC cell lines (HepG2, SMMC-7721, BEL-7402) were treated with Sal. Cell doubling time determinated by drawing growth curve, viability evaluated using Counting Kit 8. fraction CD133+ subpopulations assessed flow cytometry. We found that inhibits...
Liver fibrosis is a common pathway leading to cirrhosis and worldwide clinical issue. Astaxanthin red carotenoid pigment with antioxidant, anticancer, anti-inflammatory properties. The aim of this study was investigate the effect astaxanthin on liver its potential protective mechanisms. induced in mouse model using CCL4 (intraperitoneal injection, three times week for 8 weeks), administered everyday at doses (20, 40, 80 mg/kg). Pathological results indicated that significantly improved...
A meta-analysis was conducted to assess the effect of omega-3 fatty acid supplementation (n-3 PUFAs) in lowering liver fat, enzyme (alanine aminotransferase (ALT), aspartate (AST), and gamma-glutamyltransferase (GGT) levels), blood lipids (triglyceride (TG), total cholesterol (TC), high density lipoprotein (HDL), low (LDL)) patients with nonalcoholic disease (NAFLD) or steatohepatitis (NASH). Methods. MEDLINE/PubMed, EMBASE, Cochrane Central Register Controlled Trials, CINAHL, Science...
Background Hepatic ischemia–reperfusion injury (HIRI) remains a pivotal clinical problem after hemorrhagic shock, transplantation, and some types of toxic hepatic injury. Apoptosis autophagy play important roles in cell death during HIRI. It is also known that N-acetylcysteine (NAC) has significant pharmacologic effects on HIRI including elimination reactive oxygen species (ROS) attenuation apoptosis. However, the NAC HIRI-induced have not been reported. In this study, we evaluated apoptosis...
Hepatocellular carcinoma (HCC) is one of the few cancers with a continuous increase in incidence and mortality. Drug resistance major problem treatment HCC. In this study, two sorafenib‐resistant HCC cell lines nude mouse subcutaneously tumor model were used to explore possible mechanisms leading sorafenib resistance, investigate whether aspirin could sensitivity hepatoma cells sorafenib. The combination resulted synergistic antitumor effect against liver tumors both vitro vivo . High...
Abstract Glycolysis, as an altered cancer cell-intrinsic metabolism, is essential hallmark of cancer. Phosphofructokinase (PFK) a metabolic sensor in the glycolytic pathway and restricting substrate availability for this enzyme has been researched extensively target chemotherapy. In present study, we investigated that effects epigallocatechin-3-gallate (EGCG), active component green tea, on inhibiting cell growth inducing apoptosis by promoting shift away from glycolysis aerobic...
Nicotinamide N-methyltransferase (NNMT) is overexpressed in various human tumors and involved the development progression of several carcinomas. In breast cancer, NNMT was found to be cell lines. However, clinical relevance cancer not yet clear.NNMT expression carcinoma examined by immunohistochemistry, then, its relationship with patient clinicopathological characteristics analyzed. The effects on chemoresistance cells were assessed viability, colony formation, apoptosis assay. NNMT, SIRT1,...
Elucidation of the role different cell lineages in liver could offer avenues to drive regeneration. Previous studies showed that SOX9+ hepatocytes can differentiate into ductal cells after injuries. It is unclear whether are uni- or bipotent progenitors at a single-cell level during injury. Here, we developed genetic tracing system delineate lineage potential homeostasis and Fate-mapping data these respond specifically injuries, with some contributing substantial number cells. Clonal...
Precise, rapid, and reliable prediction of rock strength parameters is great significance for underground engineering. This paper presents a method predicting including the Poisson's ratio (P), elastic modulus (E), uniaxial compressive (UCS) based on computer drilling jumbo measurement while (MWD) data. First, distribution characteristics correlation MWD data are studied; second, filtering proposed, which reduces influence operational mechanical factors; finally, an intelligent model...
We identified a novel human AMP-activated protein kinase (AMPK) family member, designated ARK5, encoding 661 amino acids with an estimated molecular mass of 74 kDa. The putative acid sequence reveals 47, 45.8, 42.4, and 55% homology to AMPK-alpha1, AMPK-alpha2, MELK, SNARK, respectively, suggesting that it is new member the AMPK family. It has Akt phosphorylation motif at 595-600, Ser(600) was found be phosphorylated by active resulting in activation activity toward SAMS peptide, consensus...
AMP-activated protein kinases (AMPKs) are a class of serine/threonine that activated by an increase in intracellular AMP concentration.They sensitive indicator cellular energy status and have been found to promote tumor cell survival during nutrient starvation.We recently identified novel AMPK catalytic subunit family member, ARK5, whose activation is directly regulated Akt, which, turn, has reported be key player malignancy.In this study, we attempted determine whether ARK5 involved...
Gaucher disease (GD) is caused by a spectrum of genetic mutations within the gene encoding lysosomal enzyme glucocerebrosidase (GCase). These often lead to misfolded proteins that are recognized unfolded protein response system and degraded through ubiquitin–proteasome pathway. Modulating this pathway with histone deacetylase inhibitors (HDACis) has been shown improve stability in other settings. To identify mechanisms involved regulation GCase determine effects HDACis on stability, we...
To investigate the effects and mechanism of genistein on hepatocellular carcinoma. Cell counting kit-8 assays showed that at 3, 6, 9 µM had no significant cytotoxic HepG2, SMMC-7721, Bel-7402 cells. scratch Transwell identified inhibited migration three cell lines. In lines, enhanced E-cadherin α-catenin, but reduced N-cadherin Vimentin both mRNA protein levels in a dose-dependent manner. Simultaneously, treatment with suppressed epithelial-mesenchymal transition (EMT) induced by TGF-β....