A5100665869- Liver Disease Diagnosis and Treatment
- Cancer, Hypoxia, and Metabolism
- Organ Transplantation Techniques and Outcomes
- Liver physiology and pathology
- Hepatocellular Carcinoma Treatment and Prognosis
- Autophagy in Disease and Therapy
- Drug-Induced Hepatotoxicity and Protection
- Peroxisome Proliferator-Activated Receptors
- Liver Disease and Transplantation
- Metabolism, Diabetes, and Cancer
- Fibroblast Growth Factor Research
- Immune cells in cancer
- Cancer-related Molecular Pathways
- Drug Transport and Resistance Mechanisms
- Gut microbiota and health
- Genomics, phytochemicals, and oxidative stress
- Cancer, Lipids, and Metabolism
- Cell death mechanisms and regulation
- Ginger and Zingiberaceae research
- Cancer Genomics and Diagnostics
- Hedgehog Signaling Pathway Studies
- Phytochemical compounds biological activities
- NF-κB Signaling Pathways
- Advanced Glycation End Products research
- Metabolism and Genetic Disorders
Shanghai Tenth People's Hospital
2016-2024
Tongji University
2016-2024
Central Hospital of Putuo District
2024
Nanjing Medical University
2016-2020
University Medical Center
2002
Boston University
2002
Boston Medical Center
2002
Abstract The aim of this study was to investigate the effect quercetin on hepatic fibrosis, a characteristic response acute or chronic liver injury. Mice were randomized bile duct ligation (BDL) carbon tetrachloride (CCl 4 ) cirrhosis models. Quercetin (100 mg/kg 200 daily) administered by gavage for 2 weeks. Liver tissue and blood samples collected histological molecular analysis. results our experiments showed that reduced BDL CCl inhibited extracellular matrix formation, regulated...
Hepatocellular carcinoma (HCC) is a common primary malignant tumor which usually progresses to an advanced stage because of late diagnosis. Sorafenib (Sora) first line medicine for HCC; however, it has been faced with enormous resistance. Simvastatin (Sim) cholesterol-lowering drug and reported inhibit growth. The present study aims determine whether Sora Sim co-treatment can improve resistance in HCC.The HCC cell LM3 established Sora-resistant (LM3-SR) were used the relationship between...
Abstract Objective Hepatocellular carcinima is one of the most common tumors in clinic and also leading causes death from cancer worldwide. Quercetin shows significant effects on blocking development various cancers. Methods We used human hepatocellular carcinoma LM3 nude mice tumor model to assess quercetin clarify its mechanism action. collected cell line treated with different doses at time periods determined vital indexes. The liver tissues were for western boltting (WB), Hematoxylin...
Genistein is a natural isoflavone with many health benefits, including antitumour effects. Increased hypoxia-inducible factor 1 α (HIF-1α) levels and glycolysis in tumour cells are associated an increased risk of mortality, cancer progression, resistance to therapy. However, the effect genistein on HIF-1α hepatocellular carcinoma (HCC) still unclear. Cell viability, apoptosis rate, lactate production, glucose uptake were measured HCC cell lines incubation. Lentivirus-expressed transporter...
Non-alcoholic fatty liver disease (NAFLD) is considered to be one of the most common chronic diseases across worldwide. Astaxanthin (Ax) a carotenoid, and beneficial effects astaxanthin, including anti-oxidative, anti-inflammatory, anti-tumour activity, have been identified. The present study aimed elucidate protective effect astaxanthin against NAFLD its underlying mechanism.Mice were fed either high fat or chow diet, with without for up 12 weeks. L02 cells treated free acids combined...
Hepatocellular carcinoma (HCC) is one of the few cancers with a continuous increase in incidence and mortality. Drug resistance major problem treatment HCC. In this study, two sorafenib‐resistant HCC cell lines nude mouse subcutaneously tumor model were used to explore possible mechanisms leading sorafenib resistance, investigate whether aspirin could sensitivity hepatoma cells sorafenib. The combination resulted synergistic antitumor effect against liver tumors both vitro vivo . High...
The treatment for advanced primary hepatocellular carcinoma (HCC) is sorafenib (SORA), while HCC has become increasingly drug resistant with enhanced aerobic glycolysis. present study aimed to examine the chemotherapeutic effects of a flavonoid proanthocyanidin B2 (PB2) on HCC.Five kinds cell lines and LO2 were used test effect PB2 proliferation, cycle, apoptosis xenograft mouse model analyzed. Lentivirus overexpressed pyruvate kinase M2 (PKM2) or sh-PKM2 was verify target PB2. detailed...
Abstract Hepatic ischemia/reperfusion (I/R) injury, which can result in severe liver injury and dysfunction, occurs a variety of conditions such as transplantation, shock, trauma. Cell death hepatic I/R has been linked to apoptosis autophagy. Shikonin plays significant protective role injury. The purpose the present study was investigate effect shikonin on explore underlying mechanism. Mice were subjected segmental (70%) warm ischemia induce Two doses (7.5 12.5 mg/kg) administered 2 h before...
Abstract Background Liver fibrosis is a wound‐healing process of liver featured by the over‐deposition extracellular matrix (ECM) and angiogenesis. However, effective treatment lacking. Procyanidin B2 (PB2) flavonoid extract abundant in grape seeds with anti‐oxidant, anti‐inflammatory anti‐cancer properties. The present study aimed to determine effects PB2 on fibrosis. Method CCl4‐induced mouse model human hepatic stellate cell (HSC) line (LX2 cells) were used activation, ECM production...
Abstract Liver fibrosis is a necessary stage in the development of chronic liver diseases to cirrhosis. This study aims investigate anti‐fibrotic effects levo‐tetrahydropalmatine (L‐THP) on hepatic mice and cell models its underlying mechanisms. Two mouse were generated male C57 by intraperitoneal injection carbon tetrachloride (CCl4) for 2 months bile duct ligation (BDL) 14 days. Levo‐tetrahydropalmatine was administered orally at doses 20 40 mg/kg. An activated LX2 model induced TGF‐β1...
Abstract Aerobic glycolysis is a well‐known hallmark of hepatocellular carcinoma (HCC). Hence, targeting the key enzymes this pathway considered novel approach to HCC treatment. The effects sodium butyrate (NaBu), salt short‐chain fatty acid butyrate, on aerobic in cells and underlying mechanism are unknown. In present study, data obtained from cell lines with mouse xenograft model revealed that NaBu inhibited vivo vitro . induced apoptosis while inhibiting proliferation Furthermore,...
Emerging evidence shows that the Hedgehog pathway and long noncoding RNA TUG1 play pivotal roles in cell proliferation, migration, invasion tumors. However, mechanism underlying effect of hepatoma remains undefined. In present study, we showed expression was negatively correlated with microRNA (miR)-132, depletion inhibited activation vitro vivo. We functions as a competing endogenous (ceRNA) by miR-132 for binding to sonic hedgehog protein HCC, thereby suppressing signaling its tumorigenic...
Shikonin is a natural product with many activities, including anti-cancer effects. Pyruvate kinase type M2 (PKM2) plays crucial role in the growth of tumor cells. However, effect shikonin on PKM2 hepatocellular carcinoma (HCC) unclear.Cell viability, apoptosis level, glucose uptake, and lactate production were detected HCC Lentivirus-overexpressed -shRNA used to verify key target shikonin. A xenograft mouse model was detect efficacy its combination sorafenib vivo.Shikonin inhibited...
Abstract Liver fibrosis, a consequence of unhealthy modern lifestyles, has growing impact on human health, particularly in developed countries. Here, we have explored the anti‐fibrotic effects propylene glycol alginate sodium sulphate (PSS), natural extract from brown algae, fibrotic mice and cell models. Thus, established bile duct ligature carbon tetrachloride mouse models LX‐2 with or without PSS treatment. pathological sections relevant indicators serum liver tissues were examined....
The aim of this study is to confirm the hepatocellular protective functions apigenin and molecular mechanism on liver fibrosis in mice.Carbon tetrachloride (CCl4) bile duct ligature (BDL) mouse models were used investigate effects fibrosis. Sixty-six male C57 mice randomly divided into eight groups, including vehicle group, CCl4 CCl4+L-apigenin (20 mg/kg) CCl4+H-apigenin (40 sham BDL BDL+L-apigenin(20 BDL+H-apigenin(40 group. Serum enzymes (ALT AST), proteins associated with autophagy,...
Hepatic ischemia reperfusion (IR) injury (IRI) occurs during liver transplantation, hepatectomy, and hemorrhagic shock. Oleanolic acid (OA) is a natural compound with antioxidant anti-inflammatory activity that has been used to treat disorders in clinical practice for several years. Here, we investigated the effects underlying mechanisms of OA hepatic IRI. A 60-minute partial (70%) hepatic, warm, ischemic model was established BALB/c mice, two doses (30 60 mg/kg) were administered...
Background . Hepatic ischemia reperfusion (IR) injury is a common phenomenon in transplantation or trauma. The aim of the present study was to determine protective effect quercetin (QE) on hepatic IR via ERK/NF- κ B pathway. Methods Mice were randomized into sham, IR, QE100 + and QE200 groups. Quercetin administered intragastrically daily at two doses (100 mg/kg 200 mg/kg) for 5 days prior injury. expression levels liver enzymes, inflammatory cytokines, other marker proteins determined 2, 8,...
Hepatic ischemia reperfusion (IR) injury contributes to the morbidity and mortality associated with liver surgery. This study investigated protective function mechanism of propylene glycol alginate sodium sulfate (PSS), a sulfated polysaccharide, in mouse hepatic IR model. PSS (25 or 50 mg/kg) saline were injected intraperitoneally male Balb/c mice 1 h before 45 min 70% warm 2, 8, 24 reperfusion. Serum tissue samples collected for evaluation hepatocellular damage, histology, assay...
Salidroside (Sal) is a glycoside extract from Rhodiola rosea L. with anti-inflammatory, antioxidant, anticancer and cardioprotective properties. The present study explored the protective effects possible mechanisms of Sal on concanavalin A (ConA)-induced liver injury in mice. Balb/C mice were divided into five groups: Normal control (injected normal saline), ConA (25 mg/kg), (10 mg/kg) +ConA, (20 + (Sal injected 2 h prior to injection) only. serum levels enzymes, pro-inflammatory cytokines,...