Objective This study investigates whether local sequential delivery of vascular endothelial growth factor-A165 (VEGF-A165) followed by platelet-derived factor-BB (PDGF-BB) with alginate hydrogels could induce an angiogenic effect and functional improvement greater than single factors after myocardial infarction.

Cell-based therapies are attractive for revascularizing and regenerating tissues organs, but clinical trials of endothelial progenitor cell transplantation have not resulted in consistent benefit. We propose a different approach which material delivery system is used to create depot vascular cells vivo that exit over time repopulate the damaged tissue participate regeneration network. Microenvironmental conditions sufficient maintain viability outward migration outgrowth (OECs) been...

Targeting of nanoparticles to ischemic tissues was studied in a murine hindlimb model. Intravenously injected fluorescent allowed ischemia-targeted imaging muscles due increased permeability blood vessels hypoxic tissues. efficiency correlated with perfusion after induction ischemia and enhanced early stages (<7 days). Therapeutic delivery vascular endothelial growth factor (VEGF) achieved by VEGF-conjugated resulted 1.7-fold increase perfusion, as compared control mice. This work supports...

To realize the promise of three-dimensional (3D) bioprinting, it is imperative to develop bioinks that possess necessary biological and rheological characteristics for printing cell-laden tissue grafts. Alginate widely used as a bioink because its properties can be modified through precrosslinking or addition thickening agents increase resolution. However, modification alginate's physiochemical using common crosslinking affect cytocompatibility. Therefore, we evaluated printability,...

The aim was to investigate that a bio-degradable alginate and poly lactide-co-glycolide (PLG) system capable of delivering growth factors sequentially would be superior single factor delivery in promoting neovascularization improving perfusion. Three groups apoE null mice underwent unilateral hindlimb ischemia surgery received ischemic limb intramuscular injections (Blank), containing VEGF165 (VEGF), or combined with PLG microspheres PDGF-BB (VEGF/PDGF). Vascularity the assessed by...

Growth factors have been widely used in strategies to regenerate and repair diseased tissues, but current therapies that go directly from bench bedside had limited clinical success.We hypothesize engineering successful with recombinant proteins will often require specific quantitative information of the spatiotemporal role development sophisticated delivery approaches provide appropriate tissue exposures.This hypothesis was tested context therapeutic angiogenesis.An vitro model angiogenesis...

Vascular endothelial growth factor (VEGF) is a potent proangiogenic peptide and its administration has been considered as potential neuroprotective strategy following cerebral stroke. Because VEGF short half-life limited access to the brain parenchyma systemic administration, approaches are being developed deliver it directly site of infarction. In present study, was incorporated into sustained release hydrogel delivery system examine benefits in rat model ischemia. The loaded with (1 μg)...

Hydrogels with the potential to provide minimally invasive cell delivery represent a powerful tool for tissue-regeneration therapies. In this context, entrapped cells should be able escape matrix becoming more available actively participate in healing process. Here, we analyzed performance of proteolytically degradable alginate hydrogels as vehicles human mesenchymal stem (hMSC) transplantation. Alginate was modified metalloproteinase (MMP)-sensitive peptide Pro-Val-Gly-Leu-Iso-Gly (PVGLIG),...

Distinct families of multipotent heart progenitors play a central role in the generation diverse cardiac, smooth muscle and endothelial cell lineages during mammalian cardiogenesis. The identification precise paracrine signals that drive cell-fate decision these progenitors, development novel approaches to deliver vivo, are critical steps towards unlocking their regenerative therapeutic potential. Herein, we have identified family human cardiac intermediates located outflow tract early fetal...

Significance Drug delivery depots used in the clinic today are single use, with no ability to refill once exhausted of drug. Our system exploits nucleic acid complementarity drug-delivering through blood. The utility this approach is demonstrated by its inhibit tumor growth a greater extent than strategies that rely on enhanced permeability and retention alone. We anticipate our will be directly applicable therapies for many diseases, including cancer, wound healing, inflammation, drug...

Background: Vascularization underlies the success of guided bone regeneration (GBR) procedures. This study evaluates regenerative potential GBR in combination with vascular endothelial growth factor (VEGF) delivery via an injectable hydrogel system. Methods: Critical‐sized defects were created rat calvariae, and procedures performed a collagen membrane alone (control), or plus bolus VEGF, application VEGF‐releasing hydrogels (VEGF–Alg). Four 8 weeks after treatment, defect sites evaluated...

Lymphatic dysfunction is associated with the progression of many cardiovascular disorders due to their role in maintaining tissue fluid homeostasis. Promoting new lymphatic vessels (lymphangiogenesis) a promising strategy reverse these via restoring function. Vascular endothelial growth factor (VEGF) members VEGF-C and VEGF-D are both potent candidates for stimulating lymphangiogenesis, though spatial temporal control factors represents challenge developing efficient therapeutic...

Nature frequently utilizes opposing factors to create a stable activator gradient robustly control pattern formation. This study employs biomimicry approach, by delivery of both angiogenic and antiangiogenic from spatially restricted zones synthetic polymer achieve temporally in vivo. The simultaneous release the two separated agents leads sharp region that is sustained over 3 wk. Further, contradictory action level proangiogenic stimulation this region, spite significant variations...