It was reported that postnatal stem cells are present in adult tissues such as bone marrow, liver, muscle, dental pulp, and periodontal ligament. We isolated from human pulp (DPSC), ligament (PDLSC), periapical follicle (PAFSC), the surrounding mandibular marrow (MBMSC) to ascertain their properties. Immunocytochemistry proved existence of these cell populations using STRO-1 a marker. These also expressed mesenchymal (MSC) markers CD29 CD44. The showed self-renewal capabilities...

Germination timing of Arabidopsis thaliana displays strong plasticity to geographic location and seasonal conditions experienced by seeds. We identified which plastic responses were adaptive using recombinant inbred lines in a field manipulation (Kentucky, KY; Rhode Island, RI), maternal photoperiod (14-h 10-h days), season dispersal (June November). Transgressive segregation created novel genotypes that had either higher fitness or lower certain environments than parent. Natural selection...

The platelet-rich fibrin (PRF) is known as a rich source of autologous cytokines and growth factors universally used for tissue regeneration in current clinical medicine. However, the microstructure PRF has not been fully investigated nor have studied key molecules that differ from plasma. We fabricated under Choukroun's protocol produced its extract (PRFe) by freezing at -80°C. conventional histological, immunohistological staining, scanning electron microscopy images showed PRF, appearing...

Cell-based therapies are attractive for revascularizing and regenerating tissues organs, but clinical trials of endothelial progenitor cell transplantation have not resulted in consistent benefit. We propose a different approach which material delivery system is used to create depot vascular cells vivo that exit over time repopulate the damaged tissue participate regeneration network. Microenvironmental conditions sufficient maintain viability outward migration outgrowth (OECs) been...

Seasonal germination timing strongly influences lifetime fitness and can affect the ability of plant populations to colonize persist in new environments. To quantify influence seasonal environmental factors on test whether pleiotropy or close linkage are significant constraints evolution different conditions, we dispersed novel recombinant genotypes Arabidopsis thaliana into two geographic locations. decouple photoperiod during seed maturation from postdispersal season that maternal...

Abstract Although climate change-related concerns have long been raised regarding the sudden dieback of Korean fir ( Abies koreana ), event’s etiology and subsequent ecosystem processes must be explained. Our study aims to clarify continuity or transience mass mortality events within coarse woody debris (CWD) structure and, if transient, identify climatic conditions (1974–2021) that could responsible for massive dying phenomena in populations. On average, precipitation during non-growing...

Germination responses to seasonal conditions determine the environment experienced by postgermination life stages, and this ability has potential consequences for evolution of plant histories. Using recombinant inbred lines Arabidopsis thaliana, we tested whether life-history characters exhibited plasticity germination timing, timing influenced strength mode natural selection on traits, expression genetic variation traits. Adult traits strong season germination, significantly altered...

Summary Alpine plants are at high risk because of climate change. Assessing the performance alpine plant species across different altitudes is useful for predicting how they may respond to changing climate. Adaptation and plasticity early life stages particular interest since seed germination seedling establishment could be crucial life‐history environmental tracking population persistence sexually reproducing plants. To evaluate past adaptation potential accommodate future conditions, seeds...

Abstract The mutualistic relationship between alien plant species and microorganisms is proposed to facilitate or hinder invasive success, depending on whether plants can form novel associations with in the introduced habitats. However, this hypothesis has not considered seed endophytes that would move together propagules. Little information available endophytic bacteria of their effects performance. We isolated a xerophytic plant, Lactuca serriola , examined growth-promoting traits. In...

Transcriptional factor nuclear factor-kappaB (NF-κB) plays a crucial role in human breast cancer cell invasion and metastasis. The carboxyl terminus of Hsc70-interacting protein (CHIP) is U-box-type ubiquitin ligase that induces ubiquitination proteasomal degradation its substrate proteins. In this study, we investigated the CHIP NF-κB pathway MDA-MB-231 cells, highly aggressive line. We showed overexpression significantly inhibits cells. suppressed expression urokinase plasminogen activator...

We used field-collected seeds of Arabidopsis thaliana (Brassicaceae) to simulate a colonization event plants from diverse locations into common environment compare regionally "local" and "foreign" populations this historically mobile species. Life history varied among regional groups, but most variation was found within regions. While we significant differences groups for important life-history characters, did not find in performance different or groups. Rather, evidence that life...

Telomeres, comprised of short repetitive sequences, are essential for genome stability and have been studied in relation to cellular senescence aging. Telomerase, the enzyme that adds telomeric repeats chromosome ends, is maintaining overall telomere length. A lack telomerase activity mammalian somatic cells results progressive shortening telomeres with each replication event. Mammals exhibit high rates cell proliferation during embryonic juvenile stages but very little occurs adult...

Seasonal germination timing strongly influences lifetime fitness and can affect the ability of plant populations to colonize persist in new environments. To quantify influence seasonal environmental factors on test whether pleiotropy or close linkage are significant constraints evolution different conditions, we dispersed novel recombinant genotypes Arabidopsis thaliana into two geographic locations. decouple photoperiod during seed maturation from postdispersal season that maternal...

1. Natural variation in life history provides the opportunity to examine its correlated population performance. Alpine systems are considered be vulnerable climate change as high-altitude conditions predicted become more similar those at low altitude. Comparing natural populations that exhibit across altitude can provide information on how may influence demography of alpine plants under a range altitudinally or climatically variable conditions. 2. We compared abundance, demography, and...

Outer membrane vesicles (OMVs) are spherical membranous structures released by Gram-negative bacteria. Several bacterial pathogens utilize OMVs as vehicles for the delivery of virulence factors into host cells. Results our previous study on proteomic analysis revealed that isolated from Salmonella enterica serovar Typhimurium had effectors known to be translocated pathogenicity island 1 (SPI-1)-encoded type III secretion system (T3SS1) cell. In present study, immunoblot confirmed six T3SS1...

Abstract Recent studies have revealed that some bacteria can inhabit plant seeds, and they are likely founders of the bacterial community in rhizosphere or inside plants at early developmental stage. Given seedling establishment is a critical fitness component weedy species, effects seed endophytic (SEB) on performance particular interest weed ecology. Here, we characterized SEB natural populations Capsella bursa ‐ pastoris , model species The composition was evaluated using deep sequencing...

Longevity is a highly variable life history trait and its variation attributable to both genetic environmental factors. Exploring well-known factors in new model system useful approach explore taxonomic plasticity of longevity. We examined responsiveness the Daphnia pulex clone TCO potentially related interventions that have been reported extend lifespan: resveratrol dietary restriction. First, we effects on lifespan fecundity which were grown at moderate (12K cells Ankistrodesmus falcatus...

Non-typhoidal Salmonella (NTS) causes gastrointestinal infection, which is commonly self-limiting in healthy humans but may lead to invasive infection at extraintestinal sites, leading bacteremia and focal systemic infections the immunocompromised. However, a prophylactic vaccine against NTS has not yet been developed. In this work, we explored potential of ΔyjeK mutant strain as live attenuated infection. YjeK combination with YjeA required for post-translational modification elongation...

Abstract Macromolecular damage leading to cell, tissue and ultimately organ dysfunction is a major contributor aging. Intracellular reactive oxygen species (ROS) resulting from normal metabolism cause most macromolecules the mitochondria play central role in this process as they are principle source of ROS. The relationship between naturally occurring variations mitochondrial (MT) genomes correspondingly less or more ROS macromolecular that changes rate aging associated organismal decline...

Germination timing of Arabidopsis thaliana displays strong plasticity to geographic location and seasonal conditions experienced by seeds. We identified which plastic responses were adaptive using recombinant inbred lines in a field manipulation (Kentucky, KY; Rhode Island, RI), maternal photoperiod (14-h 10-h days), season dispersal (June November). Transgressive segregation created novel genotypes that had either higher fitness or lower certain environments than parent. Natural selection...