It was reported that postnatal stem cells are present in adult tissues such as bone marrow, liver, muscle, dental pulp, and periodontal ligament. We isolated from human pulp (DPSC), ligament (PDLSC), periapical follicle (PAFSC), the surrounding mandibular marrow (MBMSC) to ascertain their properties. Immunocytochemistry proved existence of these cell populations using STRO-1 a marker. These also expressed mesenchymal (MSC) markers CD29 CD44. The showed self-renewal capabilities...

The aim of this study was to evaluate the in vivo bone regeneration capability alginate (AL), AL/hydroxyapatite (HA), and AL/HA/silk fibroin (SF) composites. Forty Sprague Dawley rats were used for animal experiments. Central calvarial (diameter: 8.0 mm) defects grafted with AL, AL/HA, or AL/HA/SF. New formation evaluated by histomorphometric analysis. To demonstrate immunocompatibility each group, level tumor necrosis factor (TNF)-α expression studied immunohistochemistry (IHC) quantitative...

We investigated whether silk fibroin peptide derived from the silkworm, Bombyx mori, could inhibit inflammation and enhance anti-inflammatory activity of Tat-superoxide dismutase (Tat-SOD), which was previously reported to effectively penetrate various cells tissues exert anti-oxidative in a mouse model inflammation. Inflammation induced by topical treatment ears with 12-O-tetradecanoylphorbol-13-acetate (TPA). Histological, Western blot, reverse transcription-polymerase chain reaction data...

Abstract Silk suture material is primarily composed of silk fibroin and regarded as a non-resorbable material. It slowly degraded by proteolysis when it implanted into the body. 4-Hexylresorcinol (4HR) well-known antiseptic. In this study, biodegradability 4HR-incorporated sutures were compared to that untreated polyglactin 910 sutures, commercially available resorbable suture. exhibited anti-microbial properties. Matrix metalloproteinase (MMP) can digest wide spectrum proteins. 4HR...

Bone morphogenic protein-2/4 (BMP-2/4) is an osteoinductive protein that accelerates osteogenesis when administered to bony defects. Sericin produced by silkworms, and has a biological activity differs depending on the degumming method used. Our results indicated high molecular weight fraction of silk sericin (MW > 30 kDa) obtained via sonication had more abundant β-sheet structure than low fraction. Administration increased BMP-2/4 expression in dose-dependent manner RAW264.7 cells human...

Abstract Angiogenesis plays an important role in active inflammation and wound healing. Our results showed that silk sericin 4-hexylresorcinol (4HR) increased vascular endothelial growth factor (VEGF) expression a dose-dependent manner RAW264.7 cells. Unlike 4HR, the of hypoxia inducible factor-1α (HIF-1α) HIF-2α. Pretreatment with HIF inhibitor decreased sericin-induced increase VEGF expression. However, did not affect 4HR-induced An matrix metalloproteinase (MMP) declined Silk production...

4-Hexyl resorcinol (4HR) is an organic compound and has been used in skin care application. 4HR M2-type macrophage activator elevates vascular endothelial growth factor (VEGF) expression via the hypoxia-inducible (HIF)-independent pathway. As cells are important wound healing, human umbilical vein (HUVECs) were treated with 4HR, changes VEGF-A, -C, transforming factor-β1 (TGF-β1) investigated. The administration of increased level TGF-β1. application TGF-β1 protein also VEGF-A -C. Knockdown...

Silk fibroin, produced by the silkworm Bombyx mori, has been widely studied as a scaffold in tissue engineering. Although it shown to be slowly biodegradable, cellular responses degraded silk fibroin fragments are largely unknown. In this study, was added MG-63 cell cultures, and changes gene expression cells were screened DNA microarray analysis. Genes showing significant (2-fold) change selected their confirmed quantitative RT-PCR western blotting. results showed that alkaline phosphatase...

Sericin is a 10-to-400 kDa hydrophilic protein with high serine content and silk constituent together fibroin. It produced in the middle gland of silkworm encoded by four sericin genes. The molecular weight its biological activity vary depending on extraction method employed. Its chemical structure, terms random coil β-sheet conformations, also differs method, thereby extending applications various fields. Sericin, which was discarded textile industry past, being applied developed biomedical...

Abstract A patient had a right mandibular defect due to resection of an ameloblastoma. Previously, the been reconstructed by iliac bone graft, and subsequently, titanium mesh with xenograft was used. However, it not successfully reconstructed. For recovery mandible continuity rehabilitation jaw movement, we manufactured customized 3-dimensional implant computer-aided design manufacturing electron beam melting technology. This designed have porous body structure lingual plate. The accurately...

Silk fibroin (SF)-based materials are exposed to both natural and artificial ultraviolet (UV) light during preparation or administration. However, the effects of UV irradiation on SF films prepared under different conditions have not yet been described in detail. In this study, four with molecular weight (MW) distribution were fabricated using solutions, which by dissolving degummed for 0.5-24 h. We observed (365 nm) induced increase yellowness absorbance at 310 nm films, indicating...

Three-dimensional (3D) bioprinting holds great promise for tissue engineering, allowing cells to thrive in a 3D environment. However, the applicability of natural polymers such as silk fibroin (SF) faces hurdles due limited mechanical strength and printability. SF, derived from silkworm Bombyx mori, is emerging potential bioink its inherent physical gelling properties. research on inducing thermosensitive behavior SF-based bioinks tailoring their properties specific requirements notably...

An extracellular 45 kDa endochitosanase was purified and characterized from the culture supernatant of Bacillus sp. P16. The enzyme showed an optimum pH 5.5 temperature 60°C, stable between 4.5-10.0 under 50°C. Km Vmax were measured with a chitosan D.A. 20.2% as 0.52 mg/ml 7.71×10−6 mol/sec/mg protein, respectively. did not degrade chitin, cellulose, or starch. chitosanase digested partially N-acetylated chitosans, maximum activity for 15-30% lesser 0-15% acetylated chitosan. rapidly reduced...

Abstract To understand the osteogenic effect of middle layer silk cocoon, sericin was examined for its cellular effects associated with tumor necrosis factor-α (TNF-α) signaling in this study. The fragmented proteins mat were evaluated TNF-α expression level murine macrophages. concentration protein released from mats higher outermost and innermost layers than layers, identified as sericin. macrophages dependent on applied sericin, genes osteogenesis osteoblast-like cells TNF-α. In animal...

The effect of S-adenosylmethionine (SAM) on the production various antibiotics was investigated to determine whether SAM-dependent methylation is required in biosynthetic pathways antibiotics. Pristinamycin II(B) and granaticin do not require their biosynthesis pathways, these two increased about 2-fold when a low concentration (50 10 microM, respectively) SAM treated; contrast, oleandomycin avermectin B1a as methyl donor biosynthesis, 5-fold 6-fold, depending within certain range. We also...

Membranes are used for guided bone regeneration (GBR) in defects. Resorbable membranes of collagen or aliphatic polyesters that do not require secondary surgery removal, unlike non-resorbable membranes, have been marketed GBR. Platelet rich fibrin membrane and silk-based recently assessed as Studies conducted on resorbable with new materials to improve physical properties without any adverse inflammatory reactions. However, clinical research data remain limited. More studies needed...

Silk cocoon is composed of multiple layers. The natural silk containing all layers was cut as a rectangular shape defined total group. inner and outermost were removed from the group remained mat middle objectives this study to compare with barrier membrane for guided bone regeneration. effects these materials on cellular proliferation alkaline phosphatase (ALP) expression MG63 cells explored. For comparing regeneration ability, bilateral defects created in calvarial areas ten adult New...

The effect of S-adenosylmethionine (SAM) on the production various antibiotics was investigated to determine whether SAM-dependent methylation is required in biosynthetic pathways antibiotics. Pristinamycin IIB and granaticin do not require their biosynthesis pathways, these two increased about 2-fold when a low concentration (50 10 μM, respectively) SAM treated; contrast, oleandomycin avermectin B1a as methyl donor biosynthesis, 5-fold 6-fold, depending within certain range. We also found...

Bone disease can be associated with bone resorption by osteoclasts, and interest in the development of antiresorptive agents has recently increased. The hydrolysate silk fibroin been studied respect to such biomedical applications. In a previous study, showed indirect inhibitory effects on differentiation osteoclasts. To further evaluate effect we investigated direct osteoclastogenesis apoptosis osteoclasts induced receptor activation nuclear factor κB ligand (RANKL). inhibited RANKL-induced...

There has been considerable interest in soft lithographic patterning processing of large scale graphene sheets due to the low cost and simplicity process along with exceptional electrical or physical properties graphene. These include an extremely high carrier mobility excellent mechanical strength. Recently, a study reported that single layer grown via chemical vapor deposition (CVD) was patterned transferred target surface by controlling energy polydimethylsiloxane (PDMS) stamp. However,...

The objective of this study was to demonstrate that a silk fibroin (SF) and 4-hexylresorcinol (4-HR) incorporation membrane could be used for guided bone regeneration technique. Fourier transform infrared measurements were obtained determine change physical property SF by 4-HR incorporation. Two peri-implant defects, 3.0 × 5.0 mm (width length), prepared on the lateral side implant hole in tibia New Zealand white rabbits (n = 8). defect left unfilled control group. Silk + applied...

Facile formation of silk fibroin/gelatin-heparin hybrid hydrogels for affinity-based growth factor sequestration and release.