A5090763956- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- Viral Infectious Diseases and Gene Expression in Insects
- Studies on Chitinases and Chitosanases
- Silk-based biomaterials and applications
- Insect Resistance and Genetics
- Neurobiology and Insect Physiology Research
- Insect Utilization and Effects
- Pluripotent Stem Cells Research
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- Nuclear Structure and Function
- Insect and Arachnid Ecology and Behavior
- MicroRNA in disease regulation
- RNA modifications and cancer
- Transgenic Plants and Applications
- Tissue Engineering and Regenerative Medicine
- Cancer-related molecular mechanisms research
- Fuel Cells and Related Materials
- Neurogenesis and neuroplasticity mechanisms
- Electrospun Nanofibers in Biomedical Applications
- Virus-based gene therapy research
- Biofuel production and bioconversion
- Insect and Pesticide Research
- Invertebrate Immune Response Mechanisms
Duke Medical Center
2020-2023
Duke University Hospital
2020
Duke University
2020
Carnegie Institution for Science
2017-2018
Department of Embryology
2017-2018
Chinese University of Hong Kong
2013-2016
University of Hong Kong
2016
Zhejiang University
2010-2014
University of North Carolina at Charlotte
2014
Prince of Wales Hospital
2014
The newly developed transcription activator-like effector protein (TALE) and clustered regularly interspaced short palindromic repeats/Cas9 factors (TF) offered a powerful precise approach for modulating gene expression. In this article, we systematically investigated the potential of these new tools in activating stringently silenced pluripotency Oct4 (Pou5f1) mouse human somatic cells. First, with number TALEs sgRNAs targeting various regions promoters, found that most efficient TALE-VP64s...
We discovered that innate immunity plays an important role in the reprogramming of fibroblasts into cardiomyocytes. In this report, we define a novel retinoic acid-inducible gene 1 Yin Yang (Rig1:YY1) pathway. found fibroblast to cardiomyocyte efficacy was enhanced by specific Rig1 activators. To understand mechanism action, performed various transcriptomic, nucleosome occupancy, and epigenomic approaches. Analysis datasets indicated agonists had no effect on reprogramming-induced changes...
iPSC-derived natural killer cells (iNKs) have emerged as a promising cellular therapy, especially for the refractory or relapsed acute myeloid leukemia (R/R AML) patients, but limited research focused on chemotaxis of iNKs. Here we demonstrate that C-X-C chemokine receptor type 4 (CXCR4) is significantly reduced in iNKs, resulting impaired bone marrow (BM) infiltration, which cannot be rescued by constitutively expressed CXCR4 iPSC due to CXCR4-induced differentiation failure. To address...
Reprogramming scar fibroblasts into new heart muscle cells has the potential to restore function injured heart. However, effectiveness of reprogramming is notably low. We have recently demonstrated that (cardiomyocytes) increased by addition RNA-sensing receptor ligands. Clinical use these ligands problematic due their ability induce adverse inflammatory events. To overcome this issue, we sought determine whether synthetic analogs natural ligands, which avoid generating insults and are...
Abstract The physiological titer of molting hormones in insects depends on relative activities synthesis and degradation pathways. Ecdysone oxidase (EO) is a key enzyme the inactivation ecdysteroid. However, there are only few reports ecdysteroid its enzymes silkworm. In this study, we cloned characterized Bombyx mori EO (BmEO). BmEO cDNA contains an ORF 1,695 bp deduced protein sequence 564 amino acid residues. two functional domains glucose–methanol–choline oxidoreductase N‐terminal...
Abstract Molting in insects is regulated by molting hormones (ecdysteroids), which are also crucial to insect growth, development, reproduction, etc. Ecdysone was inactivated 3‐dehydroecdysone (3DE) under ecdysone oxidase (EO), and followed NAD(P)H‐dependent irreversible reduction 3‐epiecdysteroid 3DE 3a‐reductase. On the other hand, undergoes reversible 3 β ‐reductase hemolymph. In this article, we cloned characterized (3DE ‐reductase) different tissues developing stage from silkworm,...
Abstract The peritrophic membrane (PM) is a semi‐permeable lining of the insect midgut, broadly analogous to mucous vertebrate gut. PM proteins are important achievements for function PM. In this study, two chitin‐binding ( Bm PM‐P43 and PM‐P41) from silkworm, Bombyx mori, were identified cloned. These showed molecular mass 43 41 kDa, respectively. deduced amino acid sequences codes protein 381 residues 364 residues, containing 12 14 cysteine followed by similar domain, both them have 5 in...
Abstract Lamins are structural components of the nuclear lamina (NL) that regulate genome organization and gene expression, but mechanism remains unclear. Using Hi-C, we show lamins maintain proper interactions among topologically associated chromatin domains (TADs) not their overall architecture. Combining Hi-C with fluorescence in situ hybridization (FISH) analyses lamina-associated (LADs), reveal lamin loss causes expansion or detachment specific LADs mouse ES cells. The detached disrupt...
Abstract Engineered human induced pluripotent stem cells (hiPSC)-derived allogeneic Natural Killer (NK) are emerging as promising safe and effective off-the-shelf cell therapy drugs. Repeated dosing of NK can partly compensate the relatively shorter persistence compared with chimeric antigen receptor T (CAR-T); However, each repeat, efficacy increasingly challenged by activation host innate adaptive immunity. Thus, reducing immunogenicity holds promise for better efficacy. In this study, we...
We have shown that miR combo directly reprograms fibroblasts into cardiomyocytes. However, both in vitro and vivo, the generated cardiomyocytes are immature. Recently, we discovered cardiomyocyte maturation is enhanced by NFkB activation. While initiates reprogramming, effect on activity modest. Therefore, only has a modest maturation. now made an important discovery 5’triphosphorylation (5’ppp) of RNA molecules markedly enhance activation; leading to increased accelerated reprogramed The...
We have discovered that innate immunity plays an important role in the reprogramming of fibroblasts into cardiomyocytes. Previously, we focused on TLR3-NFkB pathway. now found evidence for alternative Rig-1:YY1 Specific Rig-1 agonists such as 3p-hpRNA were to enhance efficacy cardiac with cardiomyocyte-specific gene expression increasing by 10 20 fold (Actn2, Myh6, Tnni3; N=6; P<0.01). Similarly, formation new cardiomyocytes was increased 5 (N=3, To determine mechanism action, MNase-seq...