A5108018249- Receptor Mechanisms and Signaling
- Bioinformatics and Genomic Networks
- Neuropeptides and Animal Physiology
- Gene expression and cancer classification
- Genetic Associations and Epidemiology
- Biomedical Text Mining and Ontologies
- Pharmacological Receptor Mechanisms and Effects
- Genetics, Bioinformatics, and Biomedical Research
- RNA Research and Splicing
- Chemical Synthesis and Analysis
- Neurogenesis and neuroplasticity mechanisms
- Genetics and Neurodevelopmental Disorders
- Platelet Disorders and Treatments
- Epigenetics and DNA Methylation
- Chromosomal and Genetic Variations
- Nuclear Receptors and Signaling
- Bipolar Disorder and Treatment
- Genetic Syndromes and Imprinting
- Tryptophan and brain disorders
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Blood groups and transfusion
- Neuroscience and Neuropharmacology Research
- Molecular Biology Techniques and Applications
- MicroRNA in disease regulation
University of Michigan
2015-2025
Affiliated Hospital of Nantong University
2025
Nantong University
2025
Henan University
2024
Jiangsu University
2021-2022
University of California, San Diego
2020
Zhejiang University
2015-2019
Chinese People's Armed Police Force
2017
PLA Army Service Academy
2017
Miami University
2016
Abstract The μ, δ, and κ opioid receptors are the three main types of round in central nervous system (CNS) periphery. These peptides with which they interact important a number physiological functions, including analgesia, respiration, hormonal regulation. This study examines expression receptor mRNAs rat brain spinal cord using situ hybridization techniques. Tissue sections were hybridized 35 S‐labeled cRNA probes to μ (744–1, 064 b), δ (304–1,287 (1,351–2,124 b) receptors. Each mRNA...
A cardinal symptom of major depressive disorder (MDD) is the disruption circadian patterns. However, to date, there no direct evidence clock dysregulation in brains patients who have MDD. Circadian rhythmicity gene expression has been observed animals and peripheral human tissues, but its presence variability brain were difficult characterize. Here, we applied time-of-death analysis data from high-quality postmortem brains, examining 24-h cyclic patterns six cortical limbic regions 55...
A full-length cDNA was isolated from a rat striatal library by using low-stringency screening with two PCR fragments, one spanning transmembrane domains 3-6 of the mouse delta opioid receptor and other unidentified but homologous to brain. The novel had long open reading frame encoding protein 380 residues 59% identity topography consistent seven-helix guanine nucleotide-binding protein-coupled receptor. COS-1 cells transfected coding region this clone showed high-affinity binding kappa...
In this report we describe findings that imply dysregulation of several fibroblast growth factor (FGF) system transcripts in frontal cortical regions brains from human subjects with major depressive disorder (MDD). This altered gene expression was discovered by microarray analysis tissue MDD, bipolar, and nonpsychiatric control verified quantitative real-time PCR and, importantly, a separate cohort MDD subjects. Furthermore, show, through specific serotonin reuptake inhibitor (SSRI)-treated...
Abstract Summary: GLay provides Cytoscape users an assorted collection of versatile community structure algorithms and graph layout functions for network clustering structured visualization. High performance is achieved by dynamically linking highly optimized C to the JAVA program, which makes especially suitable decomposition, display exploratory analysis large biological networks. Availability: http://brainarray.mbni.med.umich.edu/glay/ Contact: sugang@umich.edu
We are developing a system to control G protein signaling in vivo regulate broad range of physiologic responses. Our utilizes protein-coupled peptide receptors engineered respond exclusively synthetic small molecule ligands and not their natural ligand(s). These designated RASSLs (receptor activated solely by ligand). have made two prototype that based on the human κ opioid receptor. Small drugs activate receptor nonaddictive safe administer . Binding assays reveal 200–2000-fold reductions...
Abstract Background While the accuracy and precision of deep sequencing data is significantly better than those obtained by earlier generation hybridization-based high throughput technologies, digital nature output often leads to unwarranted confidence in their reliability. Results The NGSQC ( N ext G eneration S equencing Q uality C ontrol) pipeline provides a set novel quality control measures for quickly detecting wide variety issues derived from two dimensional surfaces, regardless assay...
Using a CH4-based membrane biofilm reactor (MBfR), we studied perchlorate (ClO4–) reduction by performing anaerobic methane oxidation coupled to denitrification (ANMO-D). We focused on the effects of nitrate (NO3–) and nitrite (NO2–) surface loadings ClO4– community's mechanism for reduction. The ANMO-D reduced up 5 mg/L nondetectable level using CH4 as only electron donor carbon source when delivery was not limiting; NO3– completely well its loading ≤0.32 g N/m2-d. When limiting, inhibited...
Human endogenous retroviruses (HERVs) make up 8% of the human genome. The HERV-K (HML-2) family is most recent group these viruses to have inserted into genome, and we detected activation proviruses in blood patients with HIV-1 infection. We report that infection activates expression a novel provirus, termed K111, present multiple copies centromeres chromosomes throughout genome yet not annotated assembly. Infection or stimulation Tat protein leads K111 proviruses. as single copy chimpanzee,...
The G-protein linked signaling system (GPLS) comprises a large number of G-proteins, G protein-coupled receptors (GPCRs), GPCR ligands, and downstream effector molecules. G-proteins interact with both GPCRs effectors such as cyclic adenosine monophosphate (cAMP), phosphatidylinositols, ion channels. GPLS is implicated in the pathophysiology pharmacology major depressive disorder (MDD) bipolar (BPD). This study evaluated whether altered at transcript level. gene expression dorsolateral...
Within the large family of G-protein-coupled receptors, a picture is emerging which contrasts binding small ligands and peptides to seven-helix configuration proteins. Because its unique richness in both peptide non-peptide ligands, opioid receptor offers several advantages for achieving better understanding similarities differences ligand/receptor interactions across different classes agonists antagonists. Since multiple, naturally occurring, interact with multiple receptors varying degrees...
Neural development involves the expression of ensembles regulatory genes that control coordinate and region-specific a host other genes, resulting in unique structure, connectivity, function each brain region. Although role some specific neural has been studied detail, we have no global view orchestration spatial temporal aspects gene across multiple regions developing brain. To this end, used transcriptional profiling to examine levels 9955 hypothalamus, hippocampus, frontal cortex seven...
ABSTRACT Approximately 8% of the human genome is made up endogenous retroviral sequences. As HIV-1 Tat protein activates overall expression retrovirus type K (HERV-K) (HML-2), we used next-generation sequencing to determine which 91 currently annotated HERV-K (HML-2) proviruses are regulated by Tat. Transcriptome total RNA isolated from Tat- and vehicle-treated peripheral blood lymphocytes a healthy donor showed that significantly 26 unique proviruses, silences 12, does not alter remaining...
Human endogenous retroviruses (HERV) make up 8% of the human genome. While youngest these retroviruses, HERV-K(HML-2), termed HK2, is able to code for all viral proteins and produce virus-like particles, it not known if virus particles package transmit HK2-related sequences. Here, we analyzed capacity HK2 packaging transmitting We created an probe, Bogota, which can be packaged into viruses, transfected cells that particles. Supernatants cells, contained then were added target...
Most proinflammatory actions of C-reactive protein (CRP) are only expressed following dissociation its native pentameric assembly into monomeric form (mCRP). However, little is known about what underlies the greatly enhanced activities mCRP. Here we show that a single sequence motif, i.e. cholesterol binding (CBS; a.a. 35–47), responsible for mediating interactions mCRP with diverse ligands. The to lipoprotein component ApoB, complement C1q, extracellular matrix components fibronectin and...
It is unclear how receptor/ligand families that are evolutionarily closely related achieve functional separation. To address this question, we focus here on the newly discovered Orphanin FQ, a peptide homologous to opioid Dynorphin, and its receptor, FQ which highly receptors. In spite of high degree homology in terms both ligands receptors, there little direct cross-talk between system endogenous system. Thus, peptides show either relatively low affinity or no toward receptor; conversely,...