A5004252210- Cancer Treatment and Pharmacology
- Kruppel-like factors research
- Cellular transport and secretion
- Immune cells in cancer
- Cellular Mechanics and Interactions
- Immune Cell Function and Interaction
- Microtubule and mitosis dynamics
- Genetic Syndromes and Imprinting
- Phosphodiesterase function and regulation
- MicroRNA in disease regulation
- Cancer-related gene regulation
- Fungal and yeast genetics research
- Plant Reproductive Biology
- Acute Myeloid Leukemia Research
- Protein Kinase Regulation and GTPase Signaling
- Cancer-related Molecular Pathways
- Immunotherapy and Immune Responses
- TGF-β signaling in diseases
- Genetic and Kidney Cyst Diseases
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Biochemical and Structural Characterization
- Cancer Immunotherapy and Biomarkers
- Endoplasmic Reticulum Stress and Disease
- Glycosylation and Glycoproteins Research
- T-cell and B-cell Immunology
Duke University
2018-2024
University of Central Florida
2012-2022
Florida College
2012-2022
Center for Cancer Research
2009-2022
University of Calcutta
2014
National Centre for Biological Sciences
2014
Purdue University West Lafayette
2009-2013
Abstract Tumor-associated myeloid cells regulate tumor growth and metastasis, their accumulation is a negative prognostic factor for breast cancer. Here we find calcium/calmodulin-dependent kinase (CaMKK2) to be highly expressed within intratumoral in mouse models of cancer, demonstrate that its inhibition suppresses by increasing effector CD8 + T immune-stimulatory subsets. macrophages (TAMs) isolated from Camkk2 −/− mice higher levels chemokines involved the recruitment compared WT....
Lowe syndrome (LS) is a devastating, X-linked genetic disease characterized by the presence of congenital cataracts, profound learning disabilities and renal dysfunction. Unfortunately, children affected with LS often die early health complications including failure. Although this was first described in 1950s gene, OCRL1, identified more than 17 years ago, mechanism which Ocrl1 defects lead to LS's symptoms remains unknown. Here we show that display characteristics ciliopathy. Specifically,...
Immune checkpoint blockade (ICB) therapies have significantly prolonged patient survival across multiple tumor types, particularly in melanoma. Interestingly, sex-specific differences response to ICB been observed, with males receiving a greater benefit from than females, although the mechanism or mechanisms underlying this difference are unknown. Mining published transcriptomic data sets, we determined that ICBs is influenced by functionality of intratumoral macrophages. This puts into...
The epsins are a conserved family of endocytic adaptors essential for cell viability in yeast and embryo development higher eukaryotes. Epsins function as by recognizing ubiquitinated cargo accessory proteins contributing to network stability/regulation membrane bending. Importantly, play critical role signaling epidermal growth factor receptor downregulation the activation notch RhoGTPase pathways. In this review, we present an overview emphasize their functional importance coordinators...
Side effects of radiation therapy (RT) remain the most challenging issue for pancreatic cancer treatment. Cerium oxide nanoparticles (CONPs) are currently being tested in pre-clinical trials as an adjuvant to sensitize cells RT and protect normal tissues from harmful side effects. CONPs were not able significantly affect RT-induced DNA damage cells, thereby ruling out sensitization through increased mitotic catastrophe. However, activation c-Jun terminal kinase (JNK), a key driver apoptosis,...
Triple-negative breast cancers (TNBC) tend to become invasive and metastatic at early stages in their development. Despite some treatment successes early-stage localized TNBC, the rate of distant recurrence remains high, long-term survival outcomes remain poor. In a search for new therapeutic targets this disease, we observed that elevated expression serine/threonine kinase calcium/calmodulin (CaM)-dependent protein 2 (CaMKK2) is highly correlated with tumor invasiveness. validation studies,...
Abstract The Lowe syndrome (LS) is a life-threatening, developmental disease characterized by mental retardation, cataracts and renal failure. Although this human illness has been linked to defective function of the phosphatidylinositol 5-phosphatase, Ocrl1 (Oculo-Cerebro-Renal ofLowe protein1), mechanism which enzyme deficiency triggers not clear. known localize mainly Golgi apparatus endosomes, however it translocates plasma membrane ruffles upon cell stimulation with growth factors....
Krüppel-like factor 8 (KLF8) regulates critical gene transcription and cellular events associated with cancer. However, the role of KLF8 in cancer remains largely unknown. Here, we report a surprisingly novel for DNA repair breast cells. Comet, clonogenic, WST-1 assays showed that expression is required protecting human cells from doxorubicin-induced damage cell death. Western blotting indicated overexpression ectopic attenuated levels marker γH2A.X doxorubicin-treated PARP-1(+/+) but not...
// Tianshu Li 1, 2 , Heng Lu 1 Debarati Mukherjee Satadru K. Lahiri Chao Shen 3 Lin Yu Jihe Zhao Burnett School of Biomedical Sciences, University Central Florida College Medicine, Orlando, FL, USA Department Cancer Biology, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, Life Wuhan University, Wuhan, China Correspondence to: Zhao, e-mail: Jihe.Zhao@ucf.edu Keywords: KLF8, EGFR, microRNA141, invasion and metastasis, breast cancer Received: March 17, 2015 Accepted: May 14,...
// Debarati Mukherjee 1, * , Heng Lu Lin Yu 1 Chunjiang He 2 Satadru K. Lahiri Tianshu Li 3 Jihe Zhao Burnett School of Biomedical Sciences, University Central Florida College Medicine, Orlando, FL, USA Basic Medical Wuhan University, Wuhan, China Current address: Cleveland Clinic, Cleveland, OH, These authors contributed equally to this work Correspondence to: Zhao, e-mail: Jihe.Zhao@ucf.edu Keywords: KLF8, CXCR4, transendothelial migration, invasion, metastasis Received: November 07,...
CaMKK2 signals through AMPK-dependent and AMPK-independent pathways to trigger cellular outputs including proliferation, differentiation, migration, resulting in changes metabolism, bone mass accrual, neuronal function, hematopoiesis, immunity. CAMKK2 is upregulated tumors hepatocellular carcinoma, prostate, breast, gastric cancer, genetic deletion myeloid cells results increased antitumor immunity several syngeneic models. Validation of the biological roles has relied on or small molecule...
The epsins are a family of adaptors involved in recruiting other endocytic proteins, binding ubiquitylated cargo and induction membrane curvature. These molecules bear characteristic epsin N-terminal homology (ENTH) domain multiple peptide motifs that mediate protein-protein interactions. We have previously demonstrated the ENTH is Cdc42 signaling regulation. Here, we present evidence yeast 2 (Ent2) plays role during cell division. observed overexpression Ent2 (ENTH2), but not Ent1, promoted...
Cell polarity is essential for many cellular functions including cell division and cell-fate determination. Although RhoGTPase signaling vesicle trafficking are both required the establishment of polarity, mechanisms by which they coordinated unclear. Here, we demonstrate that yeast RhoGAP (GTPase Activating Protein), Bem3, targeted to sites polarized growth endocytic recycling pathways. Specifically, deletion SLA2 or RCY1 led mislocalization Bem3 depolarized puncta accumulation in...
Lowe syndrome (LS) is a lethal X-linked genetic disease caused by functional deficiencies of the phosphatidlyinositol 5-phosphatase, Ocrl1. In past four years, our lab described first Ocrl1-specific cellular phenotypes using dermal fibroblasts from LS patients. These phenotypes, validated in an ocrl1-morphant zebrafish model, included membrane remodeling (cell migration/spreading, fluid-phase uptake) defects and primary cilia assembly abnormalities. On one hand, findings unraveled likely to...
Karanjin, the furanoflavonoid reported to possess gastroprotective and anti-diabetic properties, was investigated against experimental arthritis its molecular signalling in inflammation explored macrophages. Karanjin isolated from hexane extract of Pongamia pinnata seeds evaluated on markers adjuvant induced model (AIA) two doses (per oral; 10 mg/kg/day 20 mg/kg/day). dose dependently reduced collagen cartilage breakdown viz. urinary hydroxyproline glucosamine, respectively, serum lysosomal...
The serine/threonine protein kinase calcium/calmodulin-dependent 2 (CAMKK2) plays critical roles in a range of biological processes. Despite its importance, only handful inhibitors CAMKK2 have been disclosed. Having selective small molecule tool to interrogate this will help demonstrate that inhibition can be therapeutically beneficial. Herein, we disclose SGC-CAMKK2-1, chemical probe targets CAMKK2.
Sorting of transmembrane proteins to various intracellular compartments depends on specific signals present within their cytosolic domains. Among these sorting signals, the tyrosine-based motif (YXXØ) is one best characterized and recognized by<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mi>μ</mml:mi></mml:math>-subunits four clathrin-associated adaptor complexes (AP-1 AP-4). Despite overlap in specificity, each<mml:math...
Abstract Calcium/calmodulin-dependent protein kinase 2 (CaMKK2) is a key regulator of energy homeostasis in several cell types. Expression this enzyme tumor cells promotes proliferation and migration, expression tumor-associated immune facilitates M2 macrophage polarization the development myeloid-derived suppressor cells. Thus, there has been interest developing CaMKK2 inhibitors as potential anticancer therapeutics. One impediment to clinical these agents that roles other cellular...
There was an error published in the ePress version of article J. Cell Sci. 122, 2453-2463.The this wrongly gave page numbers as 2454-2464.We apologise for mistake.