A5085156788- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immune cells in cancer
- Immunotherapy and Immune Responses
- HIV Research and Treatment
- Acute Myeloid Leukemia Research
- Myasthenia Gravis and Thymoma
- Monoclonal and Polyclonal Antibodies Research
- Hematopoietic Stem Cell Transplantation
- Immunodeficiency and Autoimmune Disorders
- Immune Response and Inflammation
- Cancer Immunotherapy and Biomarkers
- Protein Tyrosine Phosphatases
- Adipokines, Inflammation, and Metabolic Diseases
- Chemokine receptors and signaling
- Metabolism, Diabetes, and Cancer
- CAR-T cell therapy research
- Regulation of Appetite and Obesity
- Pancreatic function and diabetes
- Neuroinflammation and Neurodegeneration Mechanisms
- RNA Interference and Gene Delivery
- Colorectal Cancer Treatments and Studies
- Complement system in diseases
- vaccines and immunoinformatics approaches
- Galectins and Cancer Biology
University of Naples Federico II
2014-2025
Duke University Hospital
2011-2025
Duke Medical Center
2011-2025
Duke University
2014-2024
Cellular Therapeutics (United Kingdom)
2014
Federico II University Hospital
1998-2010
Hôpital Pellegrin
2006-2010
Centre Hospitalier Universitaire de Bordeaux
2007-2010
University of Campania "Luigi Vanvitelli"
2003-2007
Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele
2007
Clonal activation of CD4+ and CD8+ T lymphocytes depends on binding peptide-major histocompatibility complex (MHC) molecule complexes by their alpha/beta receptors, eventually resulting in sufficient aggregation to initiate second messenger generation. The nature intracellular signals from such cell receptor (TCR) occupancy is believed be independent the specific structure ligand being bound, vary quantitatively, not qualitatively, with concentration offered affinity for peptide-MHC complex....
Abstract Tumor-associated myeloid cells regulate tumor growth and metastasis, their accumulation is a negative prognostic factor for breast cancer. Here we find calcium/calmodulin-dependent kinase (CaMKK2) to be highly expressed within intratumoral in mouse models of cancer, demonstrate that its inhibition suppresses by increasing effector CD8 + T immune-stimulatory subsets. macrophages (TAMs) isolated from Camkk2 −/− mice higher levels chemokines involved the recruitment compared WT....
Accidental or deliberate ionizing radiation exposure can be fatal due to widespread hematopoietic destruction. However, little is known about either the course of injury molecular pathways that regulate subsequent regenerative response. Here we show Wnt signaling pathway critically important for regeneration after radiation-induced injury. Using reporter mice, triggers activation in stem and progenitor cells. β-Catenin-deficient which lack ability activate canonical signaling, exhibited...
Previous studies on the role of specific residues peptide or MHC molecule in Ag presentation have revealed sensitivity this complex system to even small changes structure. In our study, we analyzed effect amino acid substitution a major CD4+ T cell determinant (T1) HIV-1 gp160 binding and recognition context various E alpha beta class II molecules. Individual alanine substitutions at all but three positions had little no negative either by hybridoma, whereas with larger side chains often...
ABSTRACT Bone remodeling, a physiological process characterized by bone formation osteoblasts (OBs) and resorption of preexisting matrix osteoclasts (OCs), is vital for the maintenance healthy tissue in adult humans. Imbalances this result pathological conditions including osteoporosis. Owing to its initial asymptomatic nature, osteoporosis often detected only after patient has sustained significant loss or fracture. Hence, anabolic therapeutics that stimulate accrual high clinical demand....
Macrophages are essential for immune responses and maintaining tissue homeostasis, exhibiting a wide range of phenotypes depending on their microenvironment. The extracellular matrix (ECM) is vital component that provides structural support organization to tissues, with stiffness acting as key regulator macrophage behavior. Using physiologically relevant 3D stiffening hydrogel models, it found increased alone promoted polarization toward pro-regenerative phenotype, mimicking the effect...
Immune parameters were analyzed in peripheral blood mononuclear cells (PBMC) and cervical mucosa biopsy specimens of human immunodeficiency virus (HIV)—seronegative women sexually exposed to HIV (exposed seronegative [ESN]), HIV-infected women, healthy without exposure. was not detected PBMC or ESN women. However, interleukin (IL)—6, IL-10, IL-12, interferon (IFN)—γ, tumor necrosis factor (TNF)—α —β mRNA elevated women; CCR5 CXCR4 augmented specimens, but PBMC, HIV-speciflc IFN-γ—secreting...
Synthetic agonists of Toll-like receptor 9 (TLR9), a class agents that induce specific immune response, exhibit antitumor activity and are currently being investigated in cancer patients. Intriguingly, their mechanisms action on tumor growth angiogenesis still incompletely understood. We recently discovered synthetic agonist TLR9, modulatory oligonucleotide (IMO), acts by impairing epidermal factor (EGFR) signaling potently synergizes with anti-EGFR antibody cetuximab GEO human colon...
Abstract Glioblastoma (GBM) is notorious for its immunosuppressive tumor microenvironment (TME) and refractory to immune checkpoint blockade (ICB). Here, we identify calmodulin-dependent kinase 2 (CaMKK2) as a driver of ICB resistance. CaMKK2 highly expressed in pro-tumor cells associated with worsened survival patients GBM. Host CaMKK2, specifically, reduces promotes Multimodal profiling the TME reveals that several resistance-associated phenotypes. exhaustion CD8 + T expansion effector CD4...
Abstract The role of src‐family tyrosine kinases in LPS‐induced DC maturation has not been fully addressed. We show that LPS induces activation c‐Src and Lyn human DC. Inhibition these kinasesby PP1 uncoupled cytokine production from the up‐regulation costimulatory molecules, resulting still capable stimulating T cell proliferation but much less efficient inducing Th1 differentiation. This is first example a pharmacological inhibitor able to modulate capacity induce particular type immune...
Abstract The ability of the envelope glycoprotein gp120 [human immunodeficiency virus (HIV) env] to induce intracellular signals is thought contribute HIV-1 pathogenesis. In present study, we found that exposure CD4+ CD45RA+ naive T cells HIVenv results in a long-lasting hyporesponsiveness antigen stimulation. This phenomenon not dependent on CD4-mediated and also can be generated by cell soluble CD4-HIVenv complexes. analysis proximal signaling reveals does activate Lck as well...
Immature dendritic cells (iDCs) can be instructed to polarize the immune response toward a noninflammatory pathway by mediators that increase intracellular concentration of cAMP. This phenomenon is associated with ability cyclic nucleoside inhibit release pro-inflammatory cytokines without affecting differentiation process (DCs). Here we investigated cAMP modulate endotoxin signaling exposing DCs exogenous 8-bromium-cyclic AMP in presence or absence H89, selective inhibitor protein kinase A,...
<h3>Background</h3> The transcription factor FOXN1 is implicated in the differentiation of thymic and skin epithelial cells, alterations it are responsible for Nude/SCID phenotype. During a genetic counselling programme offered to couples at risk community where high frequency mutated had been documented, identification human <i>FOXN1<sup>−/−</sup></i> fetus gave unique opportunity study T cell development utero. <h3>Results</h3> Total blockage CD4<sup>+</sup> maturation severe impairment...
Myeloid-derived suppressor cells (MDSCs) are a hetero geneous group of cells, which can suppress the immune response, promote tumor progression and impair efficacy immunotherapies. Consequently, pharmacological targeting MDSC is emerging as new immunotherapeutic strategy to stimulate natural anti-tumor response potentiate Herein, we leveraged genetically modified models small molecule inhibitor validate Calcium-Calmodulin Kinase 2 (CaMKK2) druggable target control accumulation in...