A5036801058- Tissue Engineering and Regenerative Medicine
- 3D Printing in Biomedical Research
- Immune cells in cancer
- Click Chemistry and Applications
- Cellular Mechanics and Interactions
- Optical Coherence Tomography Applications
- Silk-based biomaterials and applications
- Chemical Synthesis and Analysis
- Advanced Sensor and Energy Harvesting Materials
- Ichthyology and Marine Biology
- Optical Imaging and Spectroscopy Techniques
- Advanced Electron Microscopy Techniques and Applications
- Tracheal and airway disorders
- Microtubule and mitosis dynamics
- Fibroblast Growth Factor Research
- Epigenetics and DNA Methylation
- Voice and Speech Disorders
- Nanoplatforms for cancer theranostics
- Comparative Animal Anatomy Studies
- Tendon Structure and Treatment
- Mitochondrial Function and Pathology
- Hippo pathway signaling and YAP/TAZ
- Wound Healing and Treatments
- Cancer, Hypoxia, and Metabolism
- Evolution and Paleontology Studies
Duke University
2023-2025
University of Delaware
1993-2024
Newark Hospital
2018
DuPont (United States)
2018
Macrophages are essential for immune responses and maintaining tissue homeostasis, exhibiting a wide range of phenotypes depending on their microenvironment. The extracellular matrix (ECM) is vital component that provides structural support organization to tissues, with stiffness acting as key regulator macrophage behavior. Using physiologically relevant 3D stiffening hydrogel models, it found increased alone promoted polarization toward pro-regenerative phenotype, mimicking the effect...
A new technique is described for the patterning of cell-guidance cues in synthetic extracellular matrices.
Abstract Cell migration through confining three dimensional (3D) topographies can lead to loss of nuclear envelope integrity, DNA damage, and genomic instability. Despite these detrimental phenomena, cells transiently exposed confinement do not usually die. Whether this is also true for subjected long‐term remains unclear at present. To investigate this, photopatterning microfluidics are employed fabricate a high‐throughput device that circumvents limitations previous cell models enables...
Chemical modification of engineered microenvironments surrounding living cells represents a means for directing cellular behaviors through cell–matrix interactions. Presented here is temporally controlled method modulating the properties biomimetic, synthetic extracellular matrices (ECM) during live cell culture employing rapid, bioorthogonal tetrazine ligation with trans-cyclooctene (TCO) dienophiles. This approach diffusion-controlled, cytocompatible, and does not rely on light, catalysts,...
Repeated mechanical and chemical insults cause an irreversible alteration of extracellular matrix (ECM) composition properties, giving rise to vocal fold scarring that is refractory treatment. Although it well known fibroblast activation myofibroblast the key development pathology, lack a physiologically relevant in vitro model folds impedes mechanistic investigations on how ECM cues promote differentiation. Herein, we describe bio-orthogonally cross-linked hydrogel platform recapitulates...
Crosslinked, degradable, and cell-adhesive hydrogel microfibers were synthesized via interfacial polymerization employing tetrazine ligation, an exceptionally fast bioorthogonal reaction between strained trans-cyclooctene (TCO) s-tetrazine (Tz). A hydrophobic trisTCO crosslinker homo-difunctional poly(ethylene glycol) (PEG)-based macromers with the group conjugated to PEG a stable carbamate (PEG-bisTz1) bond or labile hydrazone (PEG-bisTz2) linkage synthesized. After laying ethyl acetate...
We demonstrate in vivo monitoring of wound healing dynamics mice treated with hydrogels varying stiffness using OCT. Distinct cellular responses were visualized and tracked, revealing the potential OCT for optimizing hydrogel-based treatments.
Abstract Hypoxia elicits a multitude of tissue responses depending on the severity and duration exposure. While chronic hypoxia is shown to impact development, regeneration, cancer, understanding threats acute (i.e., short‐term) limited mainly due its transient nature. Here, novel gelatin‐dextran (Gel‐Dex) hydrogel established that decouples formation oxygen consumption thus facilitates 3D sprouting from endothelial spheroids and, subsequently, induces “on‐demand.” The Gel‐Dex platform...