A5077702152- Ovarian cancer diagnosis and treatment
- Cancer Immunotherapy and Biomarkers
- Cancer Genomics and Diagnostics
- Cancer Treatment and Pharmacology
- Endometrial and Cervical Cancer Treatments
- Genetic factors in colorectal cancer
- Thyroid Cancer Diagnosis and Treatment
- Microtubule and mitosis dynamics
- Cellular Mechanics and Interactions
- Head and Neck Anomalies
- Lung Cancer Treatments and Mutations
- Folate and B Vitamins Research
- Thyroid and Parathyroid Surgery
- PARP inhibition in cancer therapy
- Ferroptosis and cancer prognosis
- BRCA gene mutations in cancer
- Radiomics and Machine Learning in Medical Imaging
- Virus-based gene therapy research
- Cancer Mechanisms and Therapy
- Metabolism and Genetic Disorders
- RNA modifications and cancer
- Maternal and fetal healthcare
- Cancer Research and Treatments
- Cancer, Hypoxia, and Metabolism
- Immunotherapy and Immune Responses
Duke Medical Center
2018-2025
Duke University
2018-2025
LabCorp (United States)
2023-2025
Duke University Hospital
2018-2024
Weill Cornell Medicine
2023
University of Washington
2023
Children's Hospital of Eastern Ontario
2023
University of Ottawa
2023
Ottawa Hospital
2023
University of California, San Francisco
2023
Immune checkpoint inhibitor therapy has shown benefit in various cancers, but their potential endometrial cancer (EC) is unknown.Prediction of neoantigen load was performed using sequencing data from the Cancer Genome Atlas set. Evaluation tumor-infiltrating lymphocytes (TILs) and PD-1 PD-L1 expression 63 patients with EC referred to our institution. The predicted median (range) (predicted neoepitopes per sample) proportional mutational load: highest ultramutated polymerase e (POLE) tumors...
Immune checkpoint inhibitors (e.g., anti-PD-1 and anti-PD-L1 antibodies) have demonstrated remarkable efficacy against hypermutated cancers such as melanomas lung carcinomas. One explanation for this effect is that lesions harbor more tumor-specific neoantigens stimulate recruitment of an increased number tumor-infiltrating lymphocytes (TILs), which counterbalanced by overexpression immune checkpoints PD-1 or PD-L1. Given BRCA1/2-mutated high grade serous ovarian (HGSOCs) exhibit a higher...
Increased recognition of the indolent nature noninvasive follicular variant papillary thyroid carcinoma (NFVPTC) along with greater insight into molecular alterations these tumors has prompted endocrine pathologists to question whether warrant a diagnosis carcinoma. However, change in terminology would affect rates malignancy fine-needle aspiration (FNA) diagnostic categories. Therefore, aim this study was determine percentage decrease associated risk for each FNA category if NFVPTCs were no...
The term noninvasive follicular thyroid neoplasm with papillary-like nuclear features (NIFTP) has been proposed to replace variant of papillary carcinoma (FVPTC) in recognition the indolent behavior this tumor. ability differentiate NIFTP from classical (cPTC) by fine-needle aspiration (FNA) would facilitate conservative management for NIFTP. aim study was determine if can be distinguished prospectively cPTC.From June 2015 January 2016, FNAs a diagnosis "malignant" or "suspicious malignancy"...
Clear cell ovarian carcinoma (CCOC) represents a distinct histologic subtype of cancer associated with significantly worse prognosis across all stages and no effective therapeutic options. Here, we report rare but clinically important cohort CCOCs microsatellite instability (MSI) (MSI-CCOCs), which are highly immunogenic may thus be very responsive to immune checkpoint blockade. MSI exhibit higher number CD8+ TILs, CD8+/CD4+ ratio, PD-1+ TILs compared stable (MSS) high grade serous cancers,...
The majority of high-grade serous ovarian cancers (HGSCs) are deficient in homologous recombination (HR) DNA repair, most commonly due to mutations or hypermethylation the BRCA1/2 genes. We aimed discover how shape cellular phenotypes and spatial interactions tumor microenvironment. Using a highly multiplex immunofluorescence image analysis we generate proteomic data for 21 markers 124,623 single cells from 112 cores originating 31 tumors with mutation (BRCA1/2mut), 13 without alterations HR...
Patients with early-onset lysosomal storage diseases are ideal candidates for prenatal therapy because organ damage starts in utero. We report the safety and efficacy results of utero enzyme-replacement (ERT) a fetus CRIM (cross-reactive immunologic material)-negative infantile-onset Pompe's disease. The family history was positive disease cardiomyopathy two previously affected deceased siblings. After receiving ERT standard postnatal therapy, current patient had normal cardiac...
Cytosolic 10-formyltetrahydrofolate dehydrogenase (FDH, ALDH1L1) is an abundant enzyme of folate metabolism. It converts to tetrahydrofolate and CO(2) in NADP(+)-dependent reaction. We have identified a gene at chromosome locus 12q24.11 the human genome, product which has 74% sequence similarity with cytosolic FDH. This protein extra N-terminal 22 amino acid residues, predicted be mitochondrial translocation signal. Transfection COS-7 or A549 cell lines construct green fluorescent was...
BACKGROUND Thyroid nodules with atypia of undetermined significance (AUS) on fine‐needle aspiration (FNA) have a low risk malignancy that appears to vary based specific features described in the AUS diagnosis. The Afirma gene expression classifier (GEC) is molecular test designed improve preoperative stratification thyroid nodules, but its performance for different patterns has not been defined. objective this study was assess GEC results and clinical outcomes architectural (AUS‐A),...
It is now recognized that noninvasive follicular variant of papillary thyroid carcinoma (NFVPTC) a distinct subset FVPTC with an exceedingly indolent clinical course. The Afirma gene-expression classifier (GEC) helps guide clinicians in the management nodules indeterminate fine-needle aspiration (FNA) results. Thyroid surgery recommended for suspicious result, whereas observation deemed reasonable most benign result. aim this study was to confirm test detects NFVPTCs and determine how many...
Abstract Background Cancer-testis antigens (CTAs) are tumor that normally expressed in the testes but aberrantly several cancers. CTA overexpression drives metastasis and progression of lung cancer, is associated with poor prognosis. To improve cancer diagnosis, prognostic prediction, drug discovery, robust identification quantitation needed. In this study, we examined quantified co-expression CTAs to derive testis antigen burden (CTAB), a novel biomarker immunotherapy response. Methods...
Tissue-based broad molecular profiling of guideline-recommended biomarkers is advised for the therapeutic management patients with non-small cell lung cancer (NSCLC). However, practice variation can affect whether all indicated are tested. We aimed to evaluate impact common single-gene testing (SGT) on subsequent comprehensive genomic (CGP) test outcomes and results in NSCLC.
The aim of this study was to evaluate whether molecular classification prognosticates treatment response in women with endometrial cancers and intraepithelial neoplasia (EIN) treated levonorgestrel intrauterine system (LNG-IUS).Patients LNG-IUS for cancer or EIN from 2013 2018 were evaluated. Using immunohistochemistry single gene sequencing POLE, patients classified into four groups as per the Proactive Molecular Risk Classifier Endometrial (ProMisE): POLE-mutated, mismatch repair-deficient...
Purpose: Determine if molecular classification using mismatch repair (MMR) and p53 protein expression predicts recurrence-free survival (RFS) overall (OS) in endometrial cancer (EC) patients treated with chemotherapy radiation (CRT) versus (CT).Methods: GOG-0258, a phase III randomized trial (NCT00942357), compared CRT to CT. Immunohistochemistry assessed MMR status. Kaplan-Meier curves adjusted Cox models analyzed outcomes by subtype.Results: ECs classified as deficient (dMMR) (27%),...
A chemotherapy response score (CRS) system was recently described to assess the histopathologic and prognosis of patients with tubo-ovarian high-grade serous carcinoma (HGSC) receiving neoadjuvant chemotherapy. The current study performed as an independent assessment this CRS system. We retrospectively identified advanced stage HGSC who received underwent interval debulking. If available, a hemotoxylin eosin slide from omentum adnexa selected for study. Slides were independently scored by 13...