A5029499507- Lymphatic System and Diseases
- CRISPR and Genetic Engineering
- Cancer Cells and Metastasis
- Reproductive Biology and Fertility
- NF-κB Signaling Pathways
- Ubiquitin and proteasome pathways
- Hippo pathway signaling and YAP/TAZ
- Congenital heart defects research
- Sympathectomy and Hyperhidrosis Treatments
- Neuroscience and Neuropharmacology Research
- Adipose Tissue and Metabolism
- Pluripotent Stem Cells Research
- Epigenetics and DNA Methylation
- Renal and related cancers
- Cancer-related Molecular Pathways
- Stress Responses and Cortisol
- Angiogenesis and VEGF in Cancer
- Mesenchymal stem cell research
- Ion channel regulation and function
- Vascular Malformations and Hemangiomas
- Neuropeptides and Animal Physiology
- Neuroinflammation and Neurodegeneration Mechanisms
- PI3K/AKT/mTOR signaling in cancer
- Planarian Biology and Electrostimulation
- Immunotherapy and Immune Responses
University of the Ryukyus
2019-2024
Systems Biology Institute
2010-2022
The University of Tokyo
2000-2022
Tokyo University of Science
2000-2022
Targeted deletion of metabotropic glutamate receptor–subtype 1 (mGluR1) gene can cause defects in development and function the cerebellum. We introduced mGluR1α transgene into mGluR1-null mutant [mGluR1 (–/–)] mice with a Purkinje cell (PC)–specific promoter. mGluR1-rescue showed normal cerebellar long-term depression regression multiple climbing fiber innervation, events significantly impaired mGluR1 (–/–) mice. The motor coordination was rescued by this transgene, dose-dependent manner....
The proto-oncogene ras plays a critical role in cell proliferation and differentiation. However, genes are abundantly expressed the adult CNS, although neuronal cells normally do not proliferate. Recently, several lines of evidence implicated involvement Ras signaling pathway synaptic plasticity. To explore proteins we generated knock-out mice lacking H- gene then used them to study roles transmission An investigation protein phosphorylation null mutant has shown that NMDA receptor is final...
The lymphatic vasculature originates from the blood through a mechanism relying on Prox1 expression and VEGFC signalling, is separated kept separate in Syk- SLP76-dependent manner. However, by which vessels are not known. To gain an understanding of vascular partitioning, we searched for affected gene spontaneous mouse mutant exhibiting blood-filled vessels, identified null mutation Plcg2 gene, encodes phospholipase Cgamma2 (PLCgamma2), positional candidate cloning. blood-lymph shunt...
The lymphatic endothelial cell (LEC) fate decision program during development has been described. However, the mechanism underlying maintenance of differentiated LEC identity remains largely unknown. Here, we show that fibroblast growth factor 2 (FGF2) plays a fundamental role in maintaining trait. In addition to demonstrating appearance LECs expressing α-smooth muscle actin mouse lymphedematous skin vivo, found immortalised lose their characteristics and undergo endothelial-to-mesenchymal...
Mammalian Ras, which is encoded by three independent genes, has been thought to be a versatile component of intracellular signalling. However, when, where and how Ras signalling plays essential roles in development whether the genes have overlapping functions particular cells remain unclear. Here, we show that proteins dose-dependently regulate lymphatic vessel growth mice. We find hypoplasia common phenotype compound knockout mice overexpressed normal an endothelial cell lineage selectively...
Abstract A variety of stem cells are controlled by the actions multiple growth factors in vitro. However, it remains largely unclear how control proliferation and differentiation vivo. Here, we describe a novel paracrine mechanism for regulating cell niche early mammalian embryos, which involves communication between inner mass (ICM) trophectoderm, from embryonic (ES) trophoblast (TS) can be derived, respectively. It is known that ES produce fibroblast factor (FGF)4 TS bone morphogenetic...
Endothelial cells are indispensable components of the vascular system, and play pivotal roles during development in health disease. Their properties have been studied extensively by vivo analysis genetically modified mice. However, further molecular cellular phenotypes endothelial their heterogeneity at various developmental stages, beds organs has often hampered difficulties culturing mouse cells. In order to overcome these difficulties, we developed a new transgenic line expressing SV40...
Modulation of VEGFR-3 expression is important for altering lymphatic endothelial cell (LEC) characteristics during the lymphangiogenic processes that occur under developmental, physiological, and pathological conditions. However, mechanisms underlying modulation Vegfr3 gene remain largely unknown. Using genetically engineered mice LECs, we demonstrated previously Ras signaling involved not only in VEGFR-3-induced signal transduction but also expression. Here, investigated roles transcription...
Abstract CREB‐binding protein (CBP) and p300 have oncogenic properties; both co‐operate with pro‐oncogenic transcription factors downstream of Ras‐Erk signalling to support cell proliferation. By contrast, missense, truncating in‐frame mutations CBP/p300 are found frequently in some human cancers, including cutaneous squamous carcinomas that originate from epidermal keratinocytes. Data dysfunction contributes keratinocyte hyperproliferation tumourigenesis; however, the mechanism by which...
The introduction of a transgene into the genome through homologous recombination or sequence-specific enzymatic modification is key technique for producing transgenic mice. Rosa26 gene has been widely used to produce mice because transcriptionally active in various cell types and, at many developmental stages, permissive constitutive expression integrated transgenes, and dispensable normal development. However, loci other than are needed generate that harbor multiple transgenes complex...
Temporal genetic modification of mice using the ligand-inducible Cre/loxP system is an important technique that allows bypass embryonic lethal phenotypes and access to adult phenotypes. In this study, we generated a tamoxifen-inducible Cre-driver mouse strain for purpose widespread temporal Cre recombination. The new line, named CM32, expresses GFPneo-fusion gene in wide variety tissues before FLP recombination after Using FLP-recombined CM32 (CM32Δ mice) reporter lines, evaluated efficiency...
Phospholipase Cγ2 (PLCγ2)-deficient mice exhibit misconnections of blood and lymphatic vessels, male infertility. However, the cell type responsible for vascular partitioning mechanism infertility remain unknown. Accordingly, we generated a mouse line that conditionally expresses endogenous Plcg2 in Cre/loxP recombination-dependent manner, found Tie2-Cre- or Pf4-Cre-driven reactivation rescues PLCγ2-deficient from phenotype. By contrast, rescued phenotype exhibited epididymal sperm...
Human adipose-derived stromal cells (ADSCs) are an important resource for cell-based therapies. However, the dynamics of ADSCs after transplantation and their mechanisms action in recipients remain unclear. Herein, we generated genetically engineered mouse to clarify biodistribution post-transplantation status analyze role recipient mesenchymal tissue modeling. Immortalized (iADSCs) retained ADSC characteristics such as marker gene expression differentiation potential. iADSCs expressing a...