A5031768501- Cellular Mechanics and Interactions
- Microtubule and mitosis dynamics
- 3D Printing in Biomedical Research
- Hippo pathway signaling and YAP/TAZ
- Protein Kinase Regulation and GTPase Signaling
- Cellular transport and secretion
- Photoreceptor and optogenetics research
- Microfluidic and Bio-sensing Technologies
- Skin and Cellular Biology Research
- Developmental Biology and Gene Regulation
- Planarian Biology and Electrostimulation
- Reproductive Biology and Fertility
- Wnt/β-catenin signaling in development and cancer
- Pluripotent Stem Cells Research
- Image and Signal Denoising Methods
- bioluminescence and chemiluminescence research
- Polymer Surface Interaction Studies
- Advanced Polymer Synthesis and Characterization
- Mechanisms of cancer metastasis
- Photoacoustic and Ultrasonic Imaging
- Erythrocyte Function and Pathophysiology
- Advanced Image Processing Techniques
- Photosynthetic Processes and Mechanisms
- Sparse and Compressive Sensing Techniques
- RNA modifications and cancer
Centre National de la Recherche Scientifique
2009-2024
Institut Jacques Monod
2019-2024
Université Paris Cité
2019-2024
Institut Curie
2009-2020
Sorbonne Université
2015-2020
Université Paris Sciences et Lettres
2018-2020
Université Sorbonne Nouvelle
2020
Physique des Cellules et Cancers
2017-2018
Centre National pour la Recherche Scientifique et Technique (CNRST)
2018
Columbia University
2012
E-cadherin plays a key role at adherens junctions between epithelial cells, but the mechanisms controlling its assembly, maintenance, and dissociation from remain poorly understood. In particular, it is not known to what extent number of E-cadherins engaged regulated by endocytosis, or adhesive bonds redistribution within membrane pool diffusive cadherins. To determine whether cadherin levels mature are endocytosis diffusion, dynamics were quantitatively analyzed new approach combining...
Directional cell motility relies on the ability of single cells to establish a front-rear polarity and can occur in absence external cues. The initiation migration has often been attributed spontaneous polarization cytoskeleton components, while spatiotemporal evolution cell-substrate interaction forces yet be resolved. Here, we one-dimensional microfabricated assay that mimics complex vivo fibrillar environment being compatible with high-resolution force measurements, quantitative...
Abstract During cell migration, Rho GTPases spontaneously form spatial gradients that define the front and back of cells. At front, active Cdc42 forms a steep gradient whereas Rac1 more extended pattern peaking few microns away. What are mechanisms shaping these gradients, what is functional role shape gradients? Here we report, using combination optogenetics micropatterning, set by patterns activators deactivators not directly transport mechanisms. simply follows distribution Guanine...
The two Ral GTPases, RalA and RalB, have crucial roles downstream Ras oncoproteins in human cancers; particular, RalB is involved invasion metastasis. However, therapies targeting signalling are not available yet. By a novel optogenetic approach, we found that light-controlled activation of at plasma-membrane promotes the recruitment Wave Regulatory Complex (WRC) via its effector exocyst, with consequent induction protrusions invasion. We show active signals to RalGEFs (Guanine nucleotide...
Cell contraction and proliferation, matrix secretion external mechanical forces induce compression during embryogenesis tumor growth, which in turn regulate cell metabolism or differentiation. How affects tissue contractility, a hallmark of function, is however unknown. Here we apply osmotic to microtissues either mouse colon adenocarcinoma CT26 cells, NIH 3T3 fibroblasts, human primary cancer-associated fibroblasts. Microtissues are anchored flexible pillars that serve as force transducers....
In the natural and technological world, multi-agent systems strongly depend on how interactions are ruled between their individual components, proper control of time-scales synchronization is a key issue. This certainly applies to living tissues when multicellular assemblies such as epithelial cells achieve complex morphogenetic processes. epithelia, because known individually generate actomyosin contractile stress, each intercellular adhesive junction line subjected opposed stresses...
Abstract Cellular stress response contributes to epithelial defense in adaptation environment changes. Galectins play a pivotal role the regulation of this malignant cells. However, precise underlying mechanisms are largely unknown. Here we demonstrate that Galectin-3, pro and anti-apoptotic lectin, is required for setting up correct cellular by orchestrating several effects. First, Galectin-3 constitutes key post-transcriptional regulator stress-related mRNA regulons coordinating cell...
Abstract At the basis of cell shape and behavior, organization actomyosin its ability to generate forces are widely studied. However, precise regulation this contractile network in space time is unclear. Here, we study role epithelial-specific protein EpCAM, a contractility modulator, motility. We show that EpCAM required for stress fiber generation front-rear polarity acquisition at single level. In fact, participates remodeling transient zone active RhoA cortex spreading epithelial cells....
Pituitary gonadotrope cells are essential for the endocrine regulation of reproduction in vertebrates. These emerge early during embryogenesis, colonize pituitary glands and organize tridimensional networks, which believed to be crucial ensure proper fertility. However, molecular mechanisms regulating organization cell population embryogenesis remain poorly understood. In this work, we characterized target genes NEUROD1 NEUROD4 transcription factors immature αT3-1 model by silico functional...
We propose a novel total variation based image restoration method that combines first and second order functionals in structurally adaptive manner. Specifically, as opposed to recent practical combined methods, which choose the relative weight between of different derivative orders global scalar parameter, we construct functional uses spatially varying so adapt local structure. Further, known methods leave user proposed automatically computes using multi-resolution formulation. The TV...
Total Variation (TV) and related extensions have been popular in image restoration due to their robust performance wide applicability. While the original formulation is still relevant after two decades of extensive research, its that combine derivatives first second orders are now being explored for better performance, with examples Combined Order TV (COTV) Generalized (TGV). As an improvement over such multi-order convex formulations, we propose a novel non-convex regularization functional...
Abstract The dynamics of the cytoskeleton and cell shape relies on coordinated activation RhoGTPase molecular switches. Among them, Rac1 participates to orchestration in space time actin branching protrusion/retraction cycles lamellipodia at front during mesenchymal migration. Biosensor imaging has revealed a graded concentration active GTP-loaded protruding regions cell. Here, using single molecule super-resolution microscopy, we reveal an additional supramolecular organization Rac1. We...
The directed migration of cell collectives is essential in various physiological processes, such as epiboly, intestinal epithelial turnover, and convergent extension during morphogenesis well pathological events like wound healing cancer metastasis 1,2 . Collective leads to the emergence coordinated movements over multiple cells. Our current understanding emphasizes that these are mainly driven by large-scale transmission signals through adherens junctions 3,4 In this study, we show...
Abstract During migration, cells present a polarized activity that is aligned with the direction of motion. This cell polarity established by an internal molecular circuitry, without requirement extracellular cues. At heart this Rho GTPases spontaneously form spatial gradients define front and back migrating cells. cell, active Cdc42 forms steep gradient whereas Rac1 more extended pattern peaking few microns away from tip. What are mechanisms shaping these gradients, what functional role...
The dynamics of the cytoskeleton and cell shape relies on coordinated activation RhoGTPase molecular switches. Among them, Rac1 participates to orchestration in space time actin branching protrusion/retraction cycles lamellipodia at front during mesenchymal migration. Biosensor imaging has revealed a graded concentration active GTP-loaded protruding regions cell. Here, using single molecule super-resolution microscopy, we reveal an additional supramolecular organization Rac1. We find that,...
Spontaneous adsorption of poly(lysine)-g-poly(ethylene glycol) comb-like copolymers (PLL-g-PEG) is a versatile mean to coat substrates with polymer layers that resist cell adhesion. We prepared redox cleavable PLL-g-PEG switch adhesion on demand. Redox sensitivity was obtained by introducing disulfide linkers between the PLL backbone and PEG strands. This modification done alone or in combination an azide end strands enabled situ conjugations peptides fluorescent labels (by simple...