A5058845577- Cancer Cells and Metastasis
- 3D Printing in Biomedical Research
- Hippo pathway signaling and YAP/TAZ
- Innovative Microfluidic and Catalytic Techniques Innovation
- Cell Image Analysis Techniques
- Cellular Mechanics and Interactions
- Image Processing Techniques and Applications
- Protein Kinase Regulation and GTPase Signaling
- Cellular transport and secretion
- CAR-T cell therapy research
- Mechanisms of cancer metastasis
- Wnt/β-catenin signaling in development and cancer
- Plant Surface Properties and Treatments
- Endoplasmic Reticulum Stress and Disease
- Virus-based gene therapy research
- Autophagy in Disease and Therapy
- Molecular Biology Techniques and Applications
- bioluminescence and chemiluminescence research
- Cancer-related gene regulation
- Advanced Vision and Imaging
- Immunotherapy and Immune Responses
Inserm
2018-2024
Institut Curie
2018-2024
Université Paris Sciences et Lettres
2018-2023
Biotherapy of Genetic Diseases, Inflammatory Disorders and Cancers
2021
There is a compelling need for approaches to predict the efficacy of immunotherapy drugs. Tumor-on-chip technology exploits microfluidics generate 3D cell co-cultures embedded in hydrogels that recapitulate simplified tumor ecosystems. Here, we present development and validation lung tumor-on-chip platforms quickly precisely measure ex vivo effects immune checkpoint inhibitors on T cell-mediated cancer death by exploiting power live imaging advanced image analysis algorithms. The integration...
The two Ral GTPases, RalA and RalB, have crucial roles downstream Ras oncoproteins in human cancers; particular, RalB is involved invasion metastasis. However, therapies targeting signalling are not available yet. By a novel optogenetic approach, we found that light-controlled activation of at plasma-membrane promotes the recruitment Wave Regulatory Complex (WRC) via its effector exocyst, with consequent induction protrusions invasion. We show active signals to RalGEFs (Guanine nucleotide...
One of the major problems in bioimaging, often highly underestimated, is whether features extracted for a discrimination or regression task will remain valid broader set similar experiments presence unpredictable perturbations during image acquisition process. Such an issue even more important when it addressed context deep learning due to lack priori known relationship between black-box descriptors (deep features) and phenotypic properties biological entities under study. In this regard,...
The emerging tumor-on-chip (ToC) approaches allow to address biomedical questions out of reach with classical cell culture techniques: in biomimetic 3D hydrogels they partially reconstitute ex vivo the complexity tumor microenvironment and cellular dynamics involving multiple types (cancer cells, immune fibroblasts, etc .). However, a clear bottleneck is extraction interpretation rich biological information contained, sometime hidden, co-culture videos. In this work, we develop apply novel...
Autophagy is a physiological degradation process that removes unnecessary or dysfunctional components of cells. It important for normal cellular homeostasis and as response to variety stresses, such nutrient deprivation. Defects in autophagy have been linked numerous human diseases, including cancers. Cancer cells require migrate invade. Here, we study the intracellular topology this interplay between cell migration by an interdisciplinary live imaging approach which combines...
ABSTRACT There is a compelling need for new approaches to predict efficacy of immunotherapy drugs. Tumor-on-chip technology exploits microfluidics generate 3D cell co-cultures embedded in hydrogels that recapitulate immune and stromal characteristics simplified tumor ecosystem. Here, we present the development validation lung-tumor-on-chip platforms quickly precisely measure ex vivo effects check-point inhibitors on T-cell-mediated cancer death, by exploiting power live imaging advanced...
Abstract Background : The Yes-associated protein (YAP) oncoprotein has been linked to both metastasis and resistance targeted therapy of lung cancer cells. We aimed investigate the effect YAP pharmacological inhibition, using YAP/ TEA domain (TEAD) transcription factor interaction inhibitors, in chemo-resistant Methods subcellular localization, cell migration, TEAD functional transcriptional activity were investigated lines with up-regulated YAP, without YAP/TEAD inhibitors. Parental (A549)...
Generation of videomicroscopy sequences will become increasingly important in order to train and evaluate dynamic image analysis methods. The latter are crucial the study biological processes such as tumour-immune cell interactions. However, current generative models developed context natural employ either a single 3D (2D+time) convolutional neural network (CNN) based generator, which fails capture long range interactions, or two separate (spatial temporal) generators, unable faithfully...