A5084384694- 3D Printing in Biomedical Research
- Electrospun Nanofibers in Biomedical Applications
- Advanced biosensing and bioanalysis techniques
- Nanoplatforms for cancer theranostics
- Microfluidic and Bio-sensing Technologies
- RNA Interference and Gene Delivery
- Nanocluster Synthesis and Applications
- Neuroscience and Neural Engineering
- Nanoparticles: synthesis and applications
- CRISPR and Genetic Engineering
- Wound Healing and Treatments
- Advanced Nanomaterials in Catalysis
- Graphene and Nanomaterials Applications
- Biosensors and Analytical Detection
- Nanoparticle-Based Drug Delivery
- Microfluidic and Capillary Electrophoresis Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Bone Tissue Engineering Materials
- Innovation and Knowledge Management
- Supramolecular Self-Assembly in Materials
- Business Strategy and Innovation
- Cellular Mechanics and Interactions
- Tissue Engineering and Regenerative Medicine
- Digital Platforms and Economics
- Antimicrobial Peptides and Activities
National Center for Nanoscience and Technology
2016-2025
University of Chinese Academy of Sciences
2019-2024
Chinese Academy of Sciences
2012-2024
University of Jinan
2024
Center for Nano Science and Technology
2015-2021
Center for NanoScience
2015-2021
Southern University of Science and Technology
2020
National Engineering Research Center for Nanotechnology
2020
Center for Excellence in Education
2016-2017
Beijing University of Posts and Telecommunications
2010-2013
Abstract CRISPR/Cas9 system is a powerful toolbox for gene editing. However, the low delivery efficiency still big hurdle impeding its applications. Herein, we report strategy to deliver Cas9‐sgPlk‐1 plasmids (CP) by multifunctional vehicle tumor therapy. We condensed CPs on TAT peptide‐modified Au nanoparticles (AuNPs/CP, ACP) via electrostatic interactions, and coated lipids (DOTAP, DOPE, cholesterol, PEG2000‐DSPE) ACP form lipid‐encapsulated, AuNPs‐condensed CP (LACP). LACP can enter...
Abstract Pancreatic cancer is one of the deadliest human cancers, whose progression highly dependent on nervous microenvironment. The suppression gene expression nerve growth factor (NGF) may have great potential in pancreatic treatment. Here we show that gold nanocluster-assisted delivery siRNA NGF (GNC–siRNA) allows efficient silencing and GNC–siRNA complex increases stability serum, prolongs circulation lifetime blood enhances cellular uptake tumour accumulation siRNA. potently...
Abstract Skin wounds on stretchable parts of the body including elbows, knees, wrists, and nape usually undergo delayed poor healing due to interference their frequent motion. Ordinary dressings that are not flexible enough face difficulty promote wound mismatching between mechanics dressing materials wounds. In this study, an injectable, biocompatible, self‐healable, conductive material poly(3,4‐ethylenedioxythiophene): poly(styrenesulfonate)/guar slime (PPGS) is developed for with various...
Abstract The type II bacterial clustered, regularly interspaced, short palindromic repeats (CRISPR)‐Cas9 (CRISPR‐associated protein) system (CRISPR‐Cas9) is a powerful toolbox for gene‐editing, however, the nonviral delivery of CRISPR‐Cas9 to cells or tissues remains key challenge. This paper reports strategy deliver Cas9 protein and single guide RNA (sgRNA) plasmid by nanocarrier with core gold nanoclusters (GNs) shell lipids. By modifying GNs HIV‐1‐transactivator transcription peptide,...
The emerging CRISPR/Cas9 system represents a promising platform for genome editing. However, its low transfection efficiency is major problem hampering the application of gene-editing potential CRISPR/Cas9. Herein, by screening pool more than 56 kinds agents, we constructed novel polyethylene glycol phospholipid-modified cationic lipid nanoparticle (PLNP)-based delivery that can condense and encapsulate Cas9/single-guide RNA (sgRNA) plasmid (DNA) to form core–shell structure (PLNP/DNA)...
This report shows that a nanovector composed of peptide-based nanofibrous hydrogel can condense DNA to result in strong immune responses against HIV. strongly activate both humoral and cellular balanced level rarely reported previous studies, which is crucial for HIV prevention therapy. In addition, this good biosafety vitro vivo. Detailed characterizations show the structure critical dramatically improved compared existing materials. great potential efficacious vaccines be potentially used...
Bacterial infections, especially multidrug-resistant bacterial are an increasingly serious problem in the field of wound healing. Herein, cellulose (BC) decorated by 4,6-diamino-2-pyrimidinethiol (DAPT)-modified gold nanoparticles (Au-DAPT NPs) is presented as a dressing (BC-Au-DAPT nanocomposites) for treating bacterially infected wounds. BC-Au-DAPT nanocomposites have better efficacy (measured terms reduced minimum inhibition concentration) than most antibiotics...
This microfluidic flow-stretch chip integrates fluid shear stress (FSS) and cyclic stretch (CS), two major mechanical stimulations in cardiovascular systems, for cultured cells. The model can deliver FSS CS simultaneously or independently to vascular cells mimic the haemodynamic microenvironment of blood vessels vivo. By imposing FSS-only, CS-only, FSS+CS stimulation on rat mesenchymal stem human umbilical vein endothelial cells, we found alignment cellular fibers varied with cell type...
Abstract Multidrug‐resistant (MDR) bacteria pose serious threats to public health as there is currently a lack of effective and biocompatible drugs kill MDR bacteria. Here, bifunctional aggregation‐induced emission luminogen (AIEgen), triphenylethylene‐naphthalimide triazole (TriPE‐NT), reported, which capable both staining killing Gram‐positive (G+) Gram‐negative (G−) The intrinsic fluorescence generating ability the TriPE unit enables TriPE‐NT monitor drug–bacteria interactions, meanwhile,...
A self‐adjusting, blood vessel‐mimicking, multilayered tubular structure with two polymers, poly(ε‐caprolactone) (PCL) and poly( dl ‐lactide‐ co ‐glycolide) (PLGA), can keep the shape of scaffold during biodegradation. The inner layer tube expand whereas outer (PLGA) layers will shrink to maintain stability space both in vitro vivo over months. This approach be generally useful for making scaffolds that require maintenance a defined shape, based on FDA‐approved materials.
Bacterial cellulose (BC) is a kind of nanobiomaterial for tissue engineering. How the nanoscale structure BC affects skin wound repair unexplored. Here, hierarchical films and their different effects on healing were studied both in vitro vivo. The bottom side film had larger pore size, looser rougher than that top side. By using microfluidics-based model, we revealed can better promote migration cells to facilitate healing. Furthermore, full-thickness wounds Wistar rats demonstrated that,...
Bacterial cellulose (BC), a network of pure nanofibers with fine crystallinity, high mechanical strength and wet capability, good biocompatibility, is material candidate for wound dressing. Hyaluronan (HA) has obvious curative properties, promoting the healing skin tissue reducing scar formation. This study explored an "orifice plate" culture method to obtain BC samples different sizes but consistent thicknesses. Novel BC-HA nanocomposites 3-D structure were obtained through solution...
A bacterial biofilm is strongly associated with chronic infections and difficult to be eradicated, posing serious threats public health. Development of effective therapeutic strategies prevent control hospital-acquired via eradication bacteria shielded by biofilms challenging. Herein, we developed deoxyribonuclease (DNase)-functionalized gold nanoclusters (AuNCs) (DNase-AuNCs), which are capable killing Gram-positive Gram-negative bacteria, especially dispersing the surrounding biofilms. The...
With the widespread use of antibiotics, number complex infection cases caused by unknown pathogens is increasing and novel antibiotics with tunable antibacterial spectra low toxicity are highly desirable. Herein, we report that, selecting thiol or amine, two groups different binding affinities gold, as anchoring groups, phenylboronic acid can be decorated on gold nanoparticles (AuNPs) densities, which contributes to Gram-selective activities AuNPs. The AuNPs modified amine- thiol-tethered...
Abstract A high level of low‐density lipoprotein cholesterol (LDL‐C) in the blood is a major risk factor for coronary heart disease. Herein, we present triple‐targeting strategy to generate loss‐of‐function mutation Pcsk9 , which regulates plasma levels, using nanocarrier‐delivered CRISPR/Cas9 system. Nuclear localization signal (NLS)‐tagged Cas9 and ‐targeted single guide RNA (sgPcsk9) were complexed with gold nanoclusters (GNCs) modified cationic HIV‐1‐transactivating transcriptor (TAT)...
Engineering functional vascular networks in vitro is critical for tissue engineering and a variety of applications. There still general lack straightforward approaches recapitulating specific structures functions vasculature. This report describes microfluidic method that utilizes fibrillogenesis collagen liquid mold to engineer three-dimensional hydrogel. The well-controlled network demonstrates both mechanical stability perfusing solutions biocompatibility cell adhesion coverage. technique...
Bacterial cellulose (BC) membranes with shape-memory properties allow the rapid preparation of artificial small-diameter blood vessels when combined microfluidics-based patterning multiple types cells. Lyophilization a wet multilayered rolled BC tube endows it memory to recover its tubular shape after unrolling. The unrolling yields flat membrane, and subsequent endothelial cells, smooth muscle fibroblast cells is carried out by microfluidics. cell-laden membrane then rerolled into tube....
Photodynamic therapy (PDT), a noninvasive therapeutic strategy for cancer treatment, which always suffers from the low reactive oxygen species (ROS) yield of traditional organic dyes. Herein, we present lipid-encapsulated aggregation-induced emission nanoparticles (AIE NPs) that have high quantum (23%) and maximum two-photon absorption (TPA) cross-section 560 GM irradiated by near-infrared light (800 nm). The AIE NPs can serve as imaging agents spatiotemporal tumor tissues with penetration...
The replacement of dressings may cause secondary damage to the wounds; thus, real-time monitoring state wound is crucial for evaluating care processes. Herein, we report a smart dressing self-monitor residue nanomedicine on it during application. We load aminobenzeneboronic acid (ABA)-modified gold nanoclusters (A-GNCs) bacterial cellulose (BC) membranes as an antibacterial display amount residual by in situ colorimetry application remedying multi-drug-resistant (MDR) bacteria-infected...
This study investigated the effects of fine-sized pork bone biochar particles on remediating As-contaminated soil and alleviating associated phytotoxicity to rice in 50-day short-term 120-day full-life-cycle pot experiments. The addition micro-nanostructured (BC) pyrolyzed at 400 600 °C (BC400 BC600) significantly increased As-treated shoot root fresh weight by 24.4–77.6%, while simultaneously reducing tissue As accumulation 26.7–64.1% increasing content 17.1–27.1% as compared treatment....
This report shows methods to fabricate polydimethylsiloxane (PDMS) microfluidic systems for long-term (up 10 day) cell culture. Undesired bubble accumulation in channels abruptly changes the microenvironment of adherent cells and leads damage death cells. Existing trapping approaches have drawbacks such as need pause fluid flow, requirement external vacuum or pressure source, possible cytotoxicity. study reports two kinds integrated trap (IBT) which excellent properties, including simplicity...