A5069531968- Antibiotic Resistance in Bacteria
- Drug Transport and Resistance Mechanisms
- Bacterial Genetics and Biotechnology
- Geomagnetism and Paleomagnetism Studies
- Solar and Space Plasma Dynamics
- Photoreceptor and optogenetics research
- Photosynthetic Processes and Mechanisms
- Hormonal Regulation and Hypertension
- Enzyme Structure and Function
- Ionosphere and magnetosphere dynamics
- Thin-Film Transistor Technologies
- Geophysics and Gravity Measurements
- Renin-Angiotensin System Studies
- Heart Rate Variability and Autonomic Control
- Pneumocystis jirovecii pneumonia detection and treatment
- RNA and protein synthesis mechanisms
- Cancer Research and Treatments
- Tuberculosis Research and Epidemiology
- Biochemical and Molecular Research
- Antibiotics Pharmacokinetics and Efficacy
- Photochromic and Fluorescence Chemistry
- Advanced Fluorescence Microscopy Techniques
- Apelin-related biomedical research
- DNA Repair Mechanisms
- Diet and metabolism studies
Osaka University
2013-2025
Kyushu University
2006-2025
Nagoya Medical Center
2024
National Hospital Organization
2024
Shukutoku University
2023
Osaka Research Institute of Industrial Science and Technology
2010-2022
Kyushu University Hospital
2021
Ono Pharmaceutical (Japan)
2021
Teijin (Japan)
2021
Takeda (Japan)
2021
The crystal structure of bovine heart cytochrome c oxidase at 2.8 Å resolution with an R value 19.9 percent reveals 13 subunits, each different from the other, five phosphatidyl ethanolamines, three glycerols and two cholates, hemes A, copper, one magnesium, zinc. Of 3606 amino acid residues in dimer, 3560 have been converged to a reasonable by refinement. A hydrogen-bonded system, including propionate heme (heme a), part peptide backbone, imidazole ligand Cu , could provide electron...
The high resolution three-dimensional x-ray structure of the metal sites bovine heart cytochrome c oxidase is reported. Cytochrome largest membrane protein yet crystallized and analyzed at atomic resolution. Electron density distribution oxidized 2.8 Å indicates a dinuclear copper center with an unexpected similar to [2Fe-2S]-type iron-sulfur center. Previously predicted zinc magnesium have been located, former bound by nuclear encoded subunit on matrix side membrane, latter situated between...
Crystal structures of bovine heart cytochrome c oxidase in the fully oxidized, reduced, azide-bound, and carbon monoxide–bound states were determined at 2.30, 2.35, 2.9, 2.8 angstrom resolution, respectively. An aspartate residue apart from O 2 reduction site exchanges its effective accessibility to matrix aqueous phase for one cytosolic concomitantly with a significant decrease pK carboxyl group, on metal sites. The movement indicates as proton pumping site. A tyrosine acidified by...
AcrB is the major multidrug exporter in Escherichia coli. Although several substrate-entrances have been identified, specificity of these various transport paths remains unclear. Here we present evidence for a substrate channel (channel 3) from central cavity trimer, which connected directly to deep pocket without first passing switch-loop and proximal . Planar aromatic cations, such as ethidium, prefer 3 channels 1 2. The efflux through increases by targeted mutations not competition with...
Abstract RamR is a transcriptional repressor of the gene-encoding RamA protein, which controls expression multidrug efflux system genes acrAB-tolC . an important multidrug-resistance factor, however, its structure and identity molecules to it responds have been unknown. Here, we report crystal in complex with multiple drugs, including berberine, violet, dequalinium, ethidium bromide rhodamine 6G. All compounds are found interact Phe155 RamR, each compound surrounded by different amino acid...
The rapid emergence of drug-resistant microbes has recently become a major concern in the medical field. In Pseudomonas aeruginosa, one most important mechanisms underlying antibiotic resistance is MexAB-OprM system, increases this efflux system result greater to wide range drugs, and genetic mutations have been identified as contributing factor. Thus, study characterized point mexB gene that are common 39 clinical P. aeruginosa isolates obtained from Genome Database. Basic Local Alignment...
The triglyceride/high-density lipoprotein cholesterol (TG/HDL-C) index, calculated as TG divided by HDL-C, has been suggested a predictor of cardiovascular disease (CVD). We investigated the association between TG/HDL-C index and CVD events in type 2 diabetes mellitus (T2DM) patients with retinopathy hyperlipidemia but no known CVD, enrolled EMPATHY study, which compared intensive standard statin therapy (targeting LDL-C levels < 70 mg/dL ≥ 100 to 120 mg/dL, respectively). A total 4665 were...
Abstract During infection, Salmonella senses and responds to harsh environments within the host. Persistence in a bile-rich environment is important for infect small intestine or gallbladder multidrug efflux system AcrAB-TolC required bile resistance. The genes encoding this are mainly regulated by ramRA locus, which composed of divergently transcribed ramA ramR genes. acrAB tolC transcriptionally activated RamA, whose gene itself repressed RamR. RamR recognizes multiple drugs; however,...
ABSTRACT The AcrAB-TolC system in Escherichia coli is an intrinsic RND-type multidrug efflux transporter that functions as a tripartite complex of the inner membrane AcrB, outer channel TolC, and adaptor protein AcrA. Although crystal structures each component this have been elucidated, structure whole has not solved. available shown AcrB TolC function trimers, but number AcrA molecules now under debate. Disulfide chemical cross-linking experiments indicated stoichiometry AcrB-AcrA-TolC...
Abstract RND-type multidrug efflux pumps have two voluminous multisite drug-binding pockets named the proximal and distal binding pocket. High- low-molecular-mass drugs bind to these pocket, respectively. Here, we report crystal structures of MexB Pseudomonas aeruginosa bound with high-molecular-mass compounds. Contrary expectations, lauryl maltose neopentyl glycol (LMNG, MW 1,005), which is a surfactant larger than pocket-binding drugs, was found pocket: one hydrophobic alkyl chains...
Abstract An emerging group of spindle cell neoplasms harboring fusions involving NTRK or non‐ kinase genes often share characteristic S100 and/or CD34 expression; however, the diagnostic utility immunohistochemical stains is not well established in this family owing to their lack specificity. Recently, CD30 expression with gene fusions, such as , BRAF RAF1 and RET has been increasingly identified. We herein report a 10‐year‐old girl high‐grade sarcoma neck. Prior histopathological...
Nucleoside triphosphates are indispensable in numerous biological processes, with enzymes involved their biogenesis playing pivotal roles cell proliferation. Pyruvate kinase (PYK), commonly regarded as the terminal glycolytic enzyme that generates ATP tandem pyruvate, is also capable of synthesizing a wide range nucleoside from diphosphate precursors. Despite substrate promiscuity, some PYKs show preference towards specific nucleotides, suggesting an underlying mechanism for differentiating...
Two azide ions were identified, one between the Fe and Cu atoms in O(2)-reduction site other at transmembrane surface of enzyme, crystal structure azide-bound form bovine heart cytochrome c oxidase 2.9 A resolution. geometries, a mu-1,3 type geometry terminal on atom, are equally possible for an ion O(2)--reduction site. The molecule was hydrogen bonded to amide group asparagine hydroxyl tyrosine mu-1,1 geometry. antisymmetric infrared bands arising from these ions, which show essentially...
Abstract We have submitted a secular variation (SV) candidate model for the thirteenth generation of International Geomagnetic Reference Field (IGRF-13) using data assimilation scheme and magnetohydrodynamic (MHD) dynamo simulation code. This is first contribution to IGRF community from research groups in Japan. A geomagnetic field derived magnetic observatory hourly means, CHAMP Swarm-A satellite data, has been used as input scheme. adopt an ensemble-based scheme, called four-dimensional...